Maturation and germination of Phaseolus vulgaris embryonic axes in culture

Long, Sharon R.; Dale, R.M.K.; Sussex, Ian M.

doi:10.1007/bf00394978

Cited by 69 publications

(36 citation statements)

References 28 publications

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“…There is, however, a large difference in the amount of storage protein accumulated, and in the appearance of the protein storage vacuoles in these two organs. In cotyledons, phaseolin accounts for approximately 50% of the total protein present, while it comprises only about 1% of the total protein in embryonic axes (9). The level of PHA in the axis is about 10% of that found in the cotyledons of beans (4).…”

Section: Tp 25 Is An Abundant Integral Tonoplast Protein In Legume Cmentioning

confidence: 99%

An Abundant, Highly Conserved Tonoplast Protein in Seeds

Johnson¹,

Herman²,

Chrispeels³

1989

Plant Physiol.

183

169

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We have isolated the membranes of the protein storage vacuoles (protein bodies) from Phaseolus vulgaris cotyledons and purified an integral membrane protein with M, 25,000 (TP 25).Antiserum to TP 25 recognizes an abundant polypeptide in the total cell extracts of many different seeds (monocots, dicots, and a gymnosperm), and specifically labels the vacuolar membranes of thin-sectioned soybean embryonic axes and cotyledons. TP

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Section: Tp 25 Is An Abundant Integral Tonoplast Protein In Legume Cmentioning

confidence: 99%

An Abundant, Highly Conserved Tonoplast Protein in Seeds

Johnson¹,

Herman²,

Chrispeels³

1989

Plant Physiol.

183

169

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“…As the seeds mature, germination percentage increases and the seeds become less sensitive to the water potential. During maturation, the developing seed accumulates and stores proteins, lipids and carbohydrates (Long et al 1981;Rosenberg and Rinne 1986). During the desiccation stage of seed development, late-embryogenic-abundant proteins are produced, which are thought to confer desiccation and stress tolerance to seeds (Dure et al 1989).…”

mentioning

confidence: 99%

Effect of seeding date on canola seed quality and seed vigour

Gusta¹,

Johnson²,

Nesbitt³

et al. 2004

Can. J. Plant Sci.

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. 2004. Effect of seeding date on canola seed quality and seed vigour. Can. J. Plant Sci. 84: 463-471. Seedling vigour is a key critical component to produce well-established canola (Brassica napus) seedlings under less than ideal conditions in western Canada. Our objective was to determine if seed lots from late October (fall) or early spring (April) seeding dates improve seed vigour relative to mid-May (May) seeding dates. We compared seed size, seed maturity, germination percentage, and seedling emergence under controlled-environment conditions from six seed lots obtained from fall-sown (1997, 1998), April-sown (1998April-sown ( , 1999 and May-sown canola (1998May-sown canola ( , 1999 cv. Quest. On average, 80% of fall-derived seed for both years was larger than 1.7 mm compared to only 32% for May-derived canola seed. April-derived seed was intermediate. Fall and April derived seed were considered 40% mature compared to 16% for May-derived seed. In germination tests at 8°C, May derived seed germinated and emerged slower with a lower total germination percentage compared to falland April-derived seed lots. Priming reduced differences in emergence between the seed lots. Field studies at Scott, SK, sown in the fall of 1999 and 2000, and in early May of 2000 demonstrated that seed derived from fall-and April-sown canola produced higher plant densities, higher biomass at bolting, and higher seed yield than seed derived from May-sown canola. Yield was 10 to 45% less from seed derived from May-sown canola than seed derived from fall-and April-sown canola. This study established the impact of seeding date on seed quality and vigour, which in turn affected emergence, seedling vigour and yield. Also, seed vigour slowly declined within 1 yr, primarily with seed derived from the May-sown canola. Le canola issu des semences de mai donne un rendement de 10 à 45 % inférieur à celui du canola venant des graines d'automne ou d'avril. L'étude illustre bien l'incidence de la date des semis sur la qualité et la vigueur des graines, ce qui a un effet sur la levée, la vigueur des plants et le rendement. Par ailleurs, la vigueur des graines du canola semé en mai s'affaiblit à mesure que l'année progresse.Mots clés : canola, Brassica napus, vigueur (graines), semis (automne), levée, rendement (qualité) Seed germination and seedling emergence result from a sequence of biological events initiated by water imbibition followed by enzymatic metabolism of storage nutrients. All of those events are regulated by the environment and the quality of the seed. Low soil temperature and lack of available soil moisture in the spring delay and reduce seedling emergence, particularly in small seeded crops such as canola (Brassica spp.). The mean spring soil temperature in the canola production area of western Canada ranges from 5 to 13°C at a 5-cm depth. The optimal temperature for canola seed germination and emergence is 15 to 20°C (Kondra et al. 1983). Both suboptimal soil temperature and lack of soil moisture delay and reduce germination perce...

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“…The continuation of embryo development (i.e. growth and storage protein accumulation) and the inhibition of precocious germination can occur in culture in the presence of ABA and various osmotica (5,17,27,28). In some cases, the ability of the osmoticum to mimic the effect of exogenous ABA is related to an increase in embryo ABA (4).…”

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confidence: 99%

A Role for the Surrounding Fruit Tissues in Preventing the Germination of Tomato (Lycopersicon esculentum) Seeds

Berry¹,

Jd²

1992

Plant Physiol.

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During tomato seed development the endogenous abscisic acid (ABA) concentration peaks at about 50 d after pollination (DAP) and then declines at later stages (60-70 DAP) of maturation. The ABA concentration in the sheath tissue immediately surrounding the seed increases with time of development, whereas that of the locule declines. The water contents of the seed and fruit tissues are similar during early development (20)(21)(22)(23)(24)(25)(26)(27)(28)(29)(30), but decline in the seed tissues between 30 and 40 DAP. The water potential and the osmotic potential of the embryo are lower than that of the locular tissue after 35 DAP also. Seeds removed from the fruit at 30, 35, and 60 DAP and placed ex situ on 35 and 60 DAP sheath and locular tissue are prevented from germinating. Development of 30 DAP seeds is maintained or promoted by the ex situ fruit tissue with which they are in contact. Their germination is inhibited until subsequent transfer to water, and germination is normal, i.e. by radicle protrusion, and viable seedlings are produced, compared with 30 DAP seeds transferred directly to water; more of these seeds germinate, but by hypocotyl extension, and seedling viability is very poor. Isolated seeds at 35 and 60 DAP re-placed in contact with fruit tissues only germinate when transferred to water after 7 d. At 30 DAP, isolated seeds are insensitive to ABA at physiological concentrations in that they germinate as if on water, albeit by hypocotyl extension. At higher concentrations germination occurs by radicle protrusion. Osmoticum prevents germination, but there is some recovery upon subsequent transfer to water. Seeds at 35 DAP are very sensitive to ABA and exhibit little or no germination, even upon transfer to water. The response of the isolated seeds to osmoticum more closely approximates that to incubation on the ex situ fruit tissues than does their response to ABA. This is also the case for isolated 60 DAP seeds, whose germination is not prevented by ABA, but only by the osmoticum; these seeds are inhibited when in contact with ex situ fruit tissues also. It is proposed that the osmotic environment within the tissues of the tomato fruit plays a greater role than endogenous ABA in preventing precocious germination of the developing seeds.rounding seed and fruit tissues, respectively, have a role in maintaining development and inhibiting precocious germination. The continuation of embryo development (i.e. growth and storage protein accumulation) and the inhibition of precocious germination can occur in culture in the presence of ABA and various osmotica (5,17,27,28). In some cases, the ability of the osmoticum to mimic the effect of exogenous ABA is related to an increase in embryo ABA (4). This is not observed for all species or conditions used (8) and is often attributed to differences in experimental materials and protocols (i.e. embryo age, ABA concentrations, type of osmotica used). The role of the fruit tissues in maintaining developmental events and in inhibiting precocious germinati...

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Maturation and germination of Phaseolus vulgaris embryonic axes in culture

Cited by 69 publications

References 28 publications

An Abundant, Highly Conserved Tonoplast Protein in Seeds

An Abundant, Highly Conserved Tonoplast Protein in Seeds

Effect of seeding date on canola seed quality and seed vigour

A Role for the Surrounding Fruit Tissues in Preventing the Germination of Tomato (Lycopersicon esculentum) Seeds

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