maturation and fertilization of follicular oocytes obtained from calves

Kajihara, Y.; Blakewood, E.G.; Myers, Michael W.; Kometani, N.; Goto, Kaori; Godke, R.A.

doi:10.1016/0093-691x(91)90196-k

Cited by 42 publications

(24 citation statements)

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“…The results from this study showed that oocytes harvested from mature cows gave similar maturation, fertilization, and early cleavage rates; however, the percentage of the cleaving embryos reaching the expanded blastocyst and the hatched blastocysts stages in vitro was greater (P < 0.05) for mature cows over that of heifers. These observations on parity of the females are in agreement with results of other recent IVF studies (17,18). Possibly the time interval needed for oocyte maturation is different between mature cows and young heifers (Kijihara, Y, personal communication 1991).…”

Section: Resultssupporting

confidence: 91%

A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

Zhang

Denniston

Godke

1992

Journal of Tissue Culture Methods

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SUMMARY: An efficient procedure has been developed and subsequently simplified for in vitro maturation and in vitro fertilization (IVF) of bovine follicular oocytes obtained from abattoir ovaries. After in vitro fertilization, successful cleavage and in vitro development were obtained using a simple and efficient cumulus cell co-culture method, which consistently produced 45 to 50% morulae during the treatment and culture of over 6000 bovine oocytes. Embryonic cell number for IVF-derived embryos was monitored at different stages of in vitro development during cumulus cell co-culture to evaluate embryo growth and assess embryo quality.

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Section: Resultssupporting

confidence: 91%

A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

Zhang

Denniston

Godke

1992

Journal of Tissue Culture Methods

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“…Some studies indicate a lower developmental competence [1,2,6] whereas others describe capacities similar to adult oocytes [7][8][9]. The rate of blastocyst formation from juvenile oocytes is variable, and survival to term after transfer of juvenile-derived embryos is generally low [1,7,10,11]. The reasons for this high incidence of fetal loss are largely unknown.…”

Section: Introductionmentioning

confidence: 97%

Offspring from One-Month-Old Lambs: Studies on the Developmental Capability of Prepubertal Oocytes1

Ptak

Loi

Dattena

et al. 1999

Biology of Reproduction

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A wave of follicular growth in lamb ovaries occurs at about 4 weeks of age, generating a life-time peak in follicle numbers. In order to take advantage of the large number of oocytes available, and to substantially decrease the generation interval, embryos were derived from oocytes collected from 1-mo-old lambs. Animals were subjected to one of 3 regimes of hormonal stimulation: groups 1 and 2 were treated to obtain germinal vesicle-stage oocytes, and group 3 to produce mature metaphase II oocytes. Adult sheep stimulated by an appropriate dose of FSH served as control. The developmental ability of collected oocytes was evaluated by either in vivo or in vitro culture to the blastocyst stage after in vitro maturation and/or fertilization. Blastocysts were transferred immediately or after cryopreservation to suitable recipient sheep. In order to investigate the full developmental potential of these embryos, pregnancies were allowed to go to term. The results show significant differences (P < 0.001) between all experimental groups in blastocyst numbers produced. Embryos derived from group 1 animals produced the greatest number of blastocysts, under both in vivo (36. 7%), and in vitro (22.9%) culture systems. Group 2 gave lowest blastocyst production (5.0%), while group 3 yielded 13.2% blastocysts. The number of pregnant recipients carrying to term lamb-derived embryos was severely reduced for both in vivo- (2 of 9; 22.2%) and in vitro-cultured, fresh (3 of 10; 30.0%) and cryopreserved (1 of 6; 16.7%) lamb embryos. This study is the first report of the birth of live lambs derived from oocytes obtained from donors as young as 4 wk. Defects in the competence of lamb-derived embryos may account for the increased fetal loss during pregnancy and the occurrence of mummified fetuses delivered alongside normal healthy lambs.

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“…Têm-se obtido embriões viáveis, gestações e bezerros nascidos, provenientes de ovócitos de bezerras pré-púberes em diferentes idades (Kajihara et al, 1991;Armstrong et al, 1992;Revel et al, 1995;Fry et al, 1998;Bols et al, 1999;Brogliatti et al, 1999;Malard, 2000;Taneja et al, 2000). Entretanto, os ovócitos coletados de bezerras são menos competentes para se desenvolver em embriões que os de vacas (Seidel et al, 1971;Palma et al, 1993;Lévesque & Sirard, 1994;Revel et al, 1995;Damiani et al, 1995Damiani et al, , 1996Khatir et al, 1996;Kuwer et al, 1999); esta competência parece aumentar com a idade da bezerra (Presicce et al, 1997), embora Armstrong et al (1992, Irvine et al (1993) e Earl et al (1998 tenham obtido resultados semelhantes para animais pré-púberes e adultos.…”

Section: Introductionunclassified

“…Para aumentar o número de ovócitos viáveis por coleta, o desenvolvimento folicular tem sido estimulado usando-se hormônios gonadotróficos, aplicados em diferentes esquemas, dosagens e produtos, com resultados variáveis e pouco conclusivos (Kajihara et al, 1991;Stubbings et al, 1992Stubbings et al, , 1993Armstrong et al, 1992Armstrong et al, , 1994Revel et al, 1995;Looney et al, 1995;Damiani et al, 1996;Tervit et al, 1997;Presicce et al, 1997;Fry et al, 1998;Malard, 2000;Taneja et al, 2000). Já outros estudos têm optado por coletas mais freqüentes sem a estimulação hormonal (Rick et al, 1996;Presicce et al, 1997;Brogliatti et al, 1999;Majerus et al, 1999).…”

Section: Introductionunclassified

Estimulação hormonal, punção folicular transvaginal e avaliação ovocitária em bezerras pré-púberes da raça Nelore (Bos taurus indicus)

Snel-Oliveira

Pereira²,

Malagoli³

et al. 2003

R. Bras. Zootec.

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RESUMO -O uso de bezerras pré-púberes como doadoras de ovócitos oferece um considerável potencial para acelerar o ganho genético através da diminuição do intervalo de gerações, além de incrementar o aproveitamento de fêmeas bovinas de alto valor genético. Os objetivos deste estudo foram analisar o efeito da idade e do tratamento estimulatório com gonadotrofinas sobre a resposta folicular, a taxa de recuperação, a produção e a avaliação morfológica de ovócitos colhidos em bezerras da raça nelore (B. t. indicus) a partir de 10 meses de idade. As bezerras foram distribuídas aleatoriamente em três grupos (n=4) e receberam tratamentos hormonais distintos, repetidos nos mesmos animais nos três períodos, aos 10, 11 e 12 meses de idade. No dia zero (D0), todas as bezerras receberam um implante intravaginal de progesterona e, em D1, 2 mg de benzoato de estradiol intramuscular (im). Em D6, deu-se início à diferenciação dos tratamentos (T): T1-120 UI de FSH, divididos em quatro aplicações im a cada 12 horas; T2 -120 UI de FSH, em uma única aplicação subcutânea (sc), mais 300 UI de eCG sc, T3 -sem tratamento estimulatório. No D8, o implante de progesterona foi retirado e a punção folicular transvaginal, guiada por ultra-som (PFTV), foi realizada no D9. Dos 402 ovócitos coletados, 53 foram desnudados e medidos, incluindo a zona pelúcida, encontrando-se um diâmetro médio de 153,38 (8,06) µm. Não houve diferença significativa entre os tratamentos e entre as idades para quantidade de folículos ≥ 3 mm, de ovócitos recuperados e de ovócitos viáveis. A média de ovócitos recuperados e ovócitos viáveis para cada tratamento foi: T1-11,08 (6,20) e 59,58% (25,80), T2-12,75 (8,04) e 66,02% (36,71), T3 -9,66 (8,15) e 51,51% (30,69), respectivamente. O valor médio da taxa de recuperação foi de 78,70%. O uso de FSH e FSH + eCG não aumentou significantemente a quantidade de folículos ≥ 3 mm no momento da PFTV, assim como dos ovócitos recuperados e viáveis aos 10, 11 e 12 meses de idade.Palavras chave: bezerras pré-púberes, estimulação hormonal, ovócitos, punção folicular zebuíno Hormonal Stimulation and Ultrasound Guided Transvaginal Follicle Aspiration and Oocyte Recovery in Nelore (Bos taurus indicus) Prepubertal CalvesABSTRACT -The use of pre-pubertal calves as oocyte donors offers a considerable potential to increase the genetic gain lowering the generations interval, as well as increase the usage of bovine females with high genetic value. The objectives of this study were to analyze the effect of age and of the stimulatory treatment with gonadotropins in the follicular response, the recovery rate, quantity and quality of oocytes of the prepubertal Nelore (B. t. indicus) calves. Twelve Nelore prepubertal females were stimulated with gonadotrophin hormone and were submitted to ultrasound guided transvaginal oocyte recovery (OPU) at 10, 11 and 12 months of age. Before OPU the females were randomly distributed into three treatment groups (n=4), which consist of different protocols of ovarian super stimulation. For all treatments the animal...

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maturation and fertilization of follicular oocytes obtained from calves

Cited by 42 publications

References 0 publications

A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

Offspring from One-Month-Old Lambs: Studies on the Developmental Capability of Prepubertal Oocytes1

Estimulação hormonal, punção folicular transvaginal e avaliação ovocitária em bezerras pré-púberes da raça Nelore (Bos taurus indicus)

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