A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

Zhang, L.; Denniston, R.S.; Godke, R.A.

doi:10.1007/bf01409099

Cited by 30 publications

(12 citation statements)

References 31 publications

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“…Although the altered culture temperatures might adversely influence development, in vitro growth was comparable to that reported previously [28,46,60,78,[81][82][83]. More likely, the beneficial effects exerted by the increased number of mouse companions were due to the increased collective surface area of the embryos.…”

Section: Discussionsupporting

confidence: 78%

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Improved felid embryo development by group culture is maintained with heterospecific companions

Spindler

Crichton²,

Ağca

et al. 2006

Theriogenology

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Section: Discussionsupporting

confidence: 78%

“…Co-incubation has often been used as a tool to beneficially modify embryo culture medium and improve embryo development, albeit with varying success [28][29][30][31][32][33][34]. These co-culture experiments all involved somatic cells as the supportive element for embryos.…”

Section: Introductionmentioning

confidence: 99%

Improved felid embryo development by group culture is maintained with heterospecific companions

Spindler

Crichton²,

Ağca

et al. 2006

Theriogenology

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“…However, culturing embryos with somatic cells has an equivocal impact on development [31][32][33][34][35][36]. Bavister [37] has discussed the diverse species-and tissue-specific results related to somatic cell culture and has questioned the validity of results showing embryotropic effects of somatic cells.…”

Section: Introductionmentioning

confidence: 96%

Quality and Age of Companion Felid Embryos Modulate Enhanced Development by Group Culture1

Spindler¹,

Wildt²

2002

Biology of Reproduction

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For some species, embryos cultured with conspecific companions may have enhanced in vitro development compared with singletons. The objective of this study was to determine the effect of quality and age of companion embryos on single felid embryos produced by in vitro maturation or in vitro fertilization. Test oocytes (intermediate quality) were inseminated and incubated alone or with 10 embryos derived from oocytes with a high, intermediate, or low glucose uptake. The effect of relative age of companion embryos on test embryo development was also examined by insemination and incubation of test oocytes alone or with 10 conspecific embryos that were older, younger, or the same age. Test embryos coincubated with better- or equal-quality companions had better development and more cells per embryo (mean +/- SEM number, 74.9 +/- 16.9 and 40.6 +/- 8.8, respectively, Day 7; P < 0.05) than test embryos coincubated with lesser-quality companions (5.1 +/- 1.4) or alone (8.4 +/- 3.7). Intermediate-quality embryos incubated with older companions had more cells per embryo (88.3 +/- 17.0; P < 0.01) than those incubated with synchronous (49.3 +/- 12.1) or younger (29.4 +/- 6.1) embryos. The cell number of solitary embryos (9.8 +/- 3.1) was less (P < 0.05) than that of every group of test embryos incubated with companions, regardless of age. In vitro development of solitary cat embryos is improved by culture with excellent-quality conspecific companions, particularly companions of an advanced age.

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“…Only those oocytes with compact layers of cumulus cells and homogeneous cytoplasm were used, and the cumulus cells were removed at different developmental stages based on the experimental design for the study. These oocytes have routinely resulted in 90-95% maturation, 80445% fertilization, and 30-40% blastocyst development with standard procedures in our laboratories (Zhang et al, 1992;Yang et al, 1993).…”

Section: Introductionmentioning

confidence: 99%

Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro

Zhang

Jiang

Wozniak

et al. 1995

Molecular Reproduction Devel

187

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Several contemporary micromanipulation techniques, such as sperm microinjection, nuclear transfer, and gene transfer by pronuclear injection, require removal of cumulus cells from oocytes or zygotes at various stages. In humans, the cumulus cells are often removed after 15-18 hr of sperm-oocyte coincubation to assist the identification of the fertilization status. This study was designed to evaluate the function of cumulus cells during oocyte maturation, fertilization, and in vitro development in cattle. Cumulus cells were removed before and after maturation and after fertilization for 0, 7, 20, and 48 hr. The cumulus-free oocytes or embryos were cultured either alone or on cumulus cell monolayers prepared on the day of maturation culture. Percentages of oocyte maturation, fertilization, and development to cleavage, morula, and blastocyst stages and to expanding or hatched blastocysts were recorded for statistical analysis by categorical data modeling (CATMOD) procedures. Cumulus cells removed before maturation significantly reduced the rate of oocyte maturation (4-26% vs. 93-96%), fertilization (0-9% vs. 91-92%), and in vitro development at all stages evaluated. Cumulus cells removed immediately prior to in vitro fertilization (IVF) or 7 hr after IVF reduced the rates of fertilization (58-60% and 71%, respectively, vs. 91-92% for controls), cleavage development (40-47% and 53-54% vs. 74-78% for controls), and morula plus blastocyst development (15% and 24% vs. 45%, P < 0.05). Cumulus cell co-culture started at various stages had no effect on fertilization and cleavage development but significantly improved rates of embryo development to morula or blastocyst stages (P < 0.05).(ABSTRACT TRUNCATED AT 250 WORDS)

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A simple method for in vitro maturation, in vitro fertilization, and co-culture of bovine oocytes

Cited by 30 publications

References 31 publications

Improved felid embryo development by group culture is maintained with heterospecific companions

Improved felid embryo development by group culture is maintained with heterospecific companions

Quality and Age of Companion Felid Embryos Modulate Enhanced Development by Group Culture1

Cumulus cell function during bovine oocyte maturation, fertilization, and embryo development in vitro

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