Matrix Solid-Phase Dispersion Extraction and Gas Chromatographic Determination of Chloramphenicol in Muscle Tissue

Kubala-Drinčić, Helena; Bažulić, Davorin; Sapunar-Postružnik, Jasenka; Grubelić, Mirela; Stuhne, Goran

doi:10.1021/jf025939d

Cited by 38 publications

(9 citation statements)

References 14 publications

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“…With regard to poultry production, blood is available for sampling during standard activities at the processing plant; it is a simple, inexpensive, relatively constant matrix to evaluate and does not require special, complicated, or expensive preparations before analysis. Sampling blood may also eliminate many issues with specific tissue characteristics (e.g., muscle) that may complicate sample preparation, sample analysis, or both (Okerman et al, 1998;Kubala-Drincic et al, 2003;Schneider et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens

et al. 2011

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In 2005, the US Food and Drug Administration withdrew approval for use of enrofloxacin in poultry, thus effectively imposing zero tolerance for residues of this antibiotic in poultry. Conventional residue monitoring for most antibiotics, including enrofloxacin, involves removing poultry carcasses from the processing line and collecting muscle tissues for analysis. Because of the loss of valuable edible products and the difficulties and expense of sampling all the carcasses, only a small portion of carcasses are tested for violative residues. Unlike muscle tissue, blood is readily available from all birds at the beginning of processing and may be used to screen for illegal residues in all poultry carcasses. It is unknown, however, if enrofloxacin concentrations in blood are predictive of muscle concentrations. In an effort to evaluate this relationship, 156 broiler chickens, 5 wk of age, were dosed with either 25 or 50 µg/mL of enrofloxacin for 3 or 7 d, respectively, in the drinking water. Blood and muscle samples were collected at 0, 1, 3, 6, 12, and 24 h (n = 6 birds/group) during the first dosing day, every 48 h during the dosing period, and every 12 h during the withdrawal period for up to 60 h after withdrawal. Enrofloxacin residues were determined in all blood and tissue samples during the dosing periods and in most of the withdrawal period for both doses. These results support the potential to use blood to screen for illegal enrofloxacin residues in edible poultry tissues in an effort to protect the human food supply.

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Section: Discussionmentioning

confidence: 99%

The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens

et al. 2011

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“…Two principal sample pretreatment methods developed for the determination of chloramphenicol are liquid-liquid extraction (LLE) 14 and solid-phase extraction (SPE). 15 However, LLE usually requires some poisonous volatile organic solvents. SPE is an important method with good puri-cation and concentration effect.…”

Section: Introductionmentioning

confidence: 99%

On-site solid phase extraction and HPLC determination of chloramphenicol in surface water and sewage

Liu

Gao

et al. 2013

Anal. Methods

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A mini-solid phase extractor (MSPE) was designed and applied in the on-site determination of trace levels of chloramphenicol. The results showed that 1.5 g of the macroporous XAD-16 resin was suitable for capturing chloramphenicol from 500 mL of solution. Methanol was used as the eluent to desorb chloramphenicol from the resin. Chloramphenicol was determined by HPLC at 278 nm with a mobile phase of 0.01 mol L À1 oxalic acid-acetonitrile (40 : 60 v/v), the retention time was around 5.07 min. The detection scope of chloramphenicol was between 0.004 and 0.120 mg L À1 . Using the MSPE, chloramphenicol in surface water and hospital sewage was determined with recoveries of 96 to 108% and RSDs of less than 6.8%. The MSPE achieved a rapid on-site preconcentration of chloramphenicol and avoided the inconvenient transportation and storage of large volumes of water sample.

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“…Therefore, it is significantly necessary to monitor the residual chloramphenicol in water environment. There are various reported methods for the determination of CAP such as dispersive liquid-liquid microextraction [45], solidphase extraction [46], aqueous two-phase extraction [47] Based on the above, a novel organic magnetic ionic liquid based on guanidinium cation was synthesized in this study, and then a new method of magnetic ionic liquid aqueous two-phase system (MILATPs) coupled with high-performance liquid chromatography (HPLC) was established to separate effectively and determine trace amount of CAP in water environment. As the result, magnetic separation technique, organic solvent free system and rapid extraction were successfully combined together in this analytical method for the first time.…”

Section: Introductionmentioning

confidence: 99%

Magnetic ionic liquid aqueous two-phase system coupled with high performance liquid chromatography: A rapid approach for determination of chloramphenicol in water environment

Yao

2017

Journal of Chromatography A

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A novel organic magnetic ionic liquid based on guanidinium cation was synthesized and characterized. A new method of magnetic ionic liquid aqueous two-phase system (MILATPs) coupled with high-performance liquid chromatography (HPLC) was established to preconcentrate and determine trace amount of chloramphenicol (CAP) in water environment for the first time. In the absence of volatile organic solvents, MILATPs not only has the excellent properties of rapid extraction, but also exhibits a response to an external magnetic field which can be applied to assist phase separation. The phase behavior of MILATPs was investigated and phase equilibrium data were correlated by Merchuk equation. Various influencing factors on CAP recovery were systematically investigated and optimized. Under the optimal conditions, the preconcentration factor was 147.2 with the precision values (RSD%) of 2.42% and 4.45% for intra-day (n=6) and inter-day (n=6), respectively. The limit of detection (LOD) and limit of quantitation (LOQ) were 0.14ngmL and 0.42ngmL, respectively. Fine linear range of 12.25ngmL-2200ngmL was obtained. Finally, the validated method was successfully applied for the analysis of CAP in some environmental waters with the recoveries for the spiked samples in the acceptable range of 94.6%-99.72%. Hopefully, MILATPs is showing great potential to promote new development in the field of extraction, separation and pretreatment of various biochemical samples.

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Matrix Solid-Phase Dispersion Extraction and Gas Chromatographic Determination of Chloramphenicol in Muscle Tissue

Cited by 38 publications

References 14 publications

The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens

The relationship between blood and muscle samples to monitor for residues of the antibiotic enrofloxacin in chickens

On-site solid phase extraction and HPLC determination of chloramphenicol in surface water and sewage

Magnetic ionic liquid aqueous two-phase system coupled with high performance liquid chromatography: A rapid approach for determination of chloramphenicol in water environment

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