On-site solid phase extraction and HPLC determination of chloramphenicol in surface water and sewage

Abstract

A mini-solid phase extractor (MSPE) was designed and applied in the on-site determination of trace levels of chloramphenicol. The results showed that 1.5 g of the macroporous XAD-16 resin was suitable for capturing chloramphenicol from 500 mL of solution. Methanol was used as the eluent to desorb chloramphenicol from the resin. Chloramphenicol was determined by HPLC at 278 nm with a mobile phase of 0.01 mol L À1 oxalic acid-acetonitrile (40 : 60 v/v), the retention time was around 5.07 min. The detection scope of chloramphenicol was between 0.004 and 0.120 mg L À1 . Using the MSPE, chloramphenicol in surface water and hospital sewage was determined with recoveries of 96 to 108% and RSDs of less than 6.8%. The MSPE achieved a rapid on-site preconcentration of chloramphenicol and avoided the inconvenient transportation and storage of large volumes of water sample.

Chloramphenicol-activated electro-chemiluminescent behavior of BNQDs-Ru(phen)3 2+ system for ultra-sensitive sensing of chloramphenicol in pharmaceutical and milk samples

“…system for ECL detection of CAP exhibits a wider linear range and a lower detection limit, comparing with the previous results (table 1) [43][44][45][46].…”

Novel nickel(ii) phthalocyanine/reduced graphene oxide: an electrochemical sensing platform for analysis of hydroquinone and chloramphenicol in environmental samples

“…26 Hence, a substantial demand exists for superior sensitivity and selectivity in the surveillance of minute quantities of both HQN and CPC. Currently, various methodologies are employed for the detection of HQN and CPC, including high-performance liquid chromatography (HP-LC), 27,28 colorimetric approaches, 29,30 fluorescence techniques, 30,31 surface-enhanced Raman scattering (SERS), 32,33 gas chromatography-mass spectrometry (GC-MS), 34,35 and capillary electrophoresis (CE). 36,37 These techniques, characterized by their high costs, operational complexity, diminished sensitivity, instability, and prolonged detection times for HQN and CPC in actual samples, impose constraints on their utility in the precise quantification of HQN and CPC.…”