Matrix metalloproteinase secretion is stimulated by TGF-ß in cultured lens epithelial cells

Richiert, Dawn M.; Ireland, Mark E.

doi:10.1076/ceyr.19.3.269.5316

Cited by 26 publications

(20 citation statements)

References 24 publications

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“…. These results are consistent with those of others [21], who reported that the secretion of pro-MMP-2 and active MMP-2 was stimulated by TGF-β. We did not observe any activity of 80 kDa or 92 kDa gelatinase, unlike Richiert and Ireland [21].…”

Section: Expression Of Mmp Mrnas Following Treatment With Tgf-βsupporting

confidence: 94%

“…These results are consistent with those of others [21], who reported that the secretion of pro-MMP-2 and active MMP-2 was stimulated by TGF-β. We did not observe any activity of 80 kDa or 92 kDa gelatinase, unlike Richiert and Ireland [21]. We used a human LEC line for the present study ; in contrast, Richiert and Ireland used a primary culture of chicken annular pad cells [21].…”

Section: Expression Of Mmp Mrnas Following Treatment With Tgf-βsupporting

confidence: 94%

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Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells

et al. 2001

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Section: Expression Of Mmp Mrnas Following Treatment With Tgf-βsupporting

confidence: 94%

Section: Expression Of Mmp Mrnas Following Treatment With Tgf-βsupporting

confidence: 94%

Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells

et al. 2001

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“…The induction of MMP-9 in the ASC of rats was also confirmed recently at the mRNA level with laser capture microdissection and real-time quantitative PCR [32]. Finally, TGF-β has also been shown to stimulate secretion of MMP-2 and MMP-9 in cultured annular pad cells of the chick lens [33].…”

Section: Matrix Metalloproteinase Expression In the Lens And Cataractssupporting

confidence: 53%

Matrix metalloproteinases as mediators of primary and secondary cataracts

West‐Mays

Pino

2007

Expert Review of Ophthalmology

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The matrix metalloproteinases (MMPs) are a family of endopeptidases involved in numerous remodeling and fibrotic disorders. Although MMPs have been shown to play important roles in regenerative and disease processes in many parts of the eye, including the cornea, retina and trabecular meshwork, the role of MMPs in the normal and cataractous lens has only recently been studied. These investigations have shown that multiple MMPs are expressed in the lens and their expression is altered in a number of cataract phenotypes. However, anterior subcapsular cataract and posterior capsular opacification, cataracts of a fibrotic nature, show a particular involvement of MMPs. This review will highlight recent findings that suggest a causative role for MMPs in these fibrotic cataract phenotypes. Keywords cataract; EMT; fibrosis; lens; MMPIs; matrix metalloproteinase Cataract is the leading cause of blindness worldwide despite the availability of effective surgery in the developed countries [1,2]. The etiology of cataract is diverse (see Allen [3] for review) with the majority of cataracts related to aging. Extracapsular cataract extraction provides quick restoration of vision and is the most frequently performed surgical procedure in the developed world, costing over US$3.5 billion each year in the USA alone [4]. However, it is not without its problems and can lead to complications such as the development of secondary cataract (up to 40-50% incidence of patients), also known as posterior capsular opacification (PCO) [5,6]. PCO is a major medical problem with profound consequences to the patient's well-being and is a significant financial burden. PCO is considered a proliferative, fibrotic disorder that involves the aberrant deposition of matrix and wrinkling of the lens capsule [7]. Another cataract type, also of a fibrotic nature, is anterior subcapsular cataract (ASC) [6,8,9]. ASC, unlike PCO, is a primary cataract that occurs when lens epithelial cells (LECs), in situ, are stimulated to transition into myofibroblasts. These fibroblasts form subcapsular plaques directly beneath the lens capsule. Despite these differences, recent evidence has shown that in both cataract phenotypes the matrix metalloproteinases (MMPs), matrix-degrading enzymes, †Author

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“…8 The regulation of this induction could include matrix components and growth factors. The interaction between the cells and their ECM is now accepted as an important element in the regulation and function of all cell types.…”

Section: Discussionmentioning

confidence: 99%

“…10 Moreover, MMPs of culturing chick lens annular pad (CLAP) cells was not detected using zymography on individual components of capsule matrix such as laminin, fibronectin, and collagen type IV. 8 Although these individual matrix components are important in cell regulation, they do not truly reflect the full complement and structural organization of the lens capsule. In this study, we examined human lens epithelia cells attached to their natural matrix.…”

Section: Discussionmentioning

confidence: 99%

Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag

Wang

2007

Eye

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Aim of purpose To observe the different expression of matrix metalloproteinases (MMPs) between pre-and postoperation of sham cataract surgery in vitro human lens capsule bag model from the same donor eye in order to investigate a possible role of MMPs in posterior capsule opacification (PCO). Methods Sham cataract surgeries were performed in six human donor eyes. Immunohistochemical staining was used to detect the expression of MMP-2 and -9 of human lens epithelial cells (LECs) on the anterior capsulorhexis. LEC migration on posterior capsule of human lens from the same donor eye was observed in a modified capsule bag model without pin. Total MMP-2 and -9 protein production were determined by enzyme-linked immunosorbent assay at days 2, 10, 20, and 30 postoperation, respectively. Results MMP-2 and -9 could not be detected immunohistochemically on the anterior capsulorhexis preoperation of cataract. Lens epithelia cells at the equator began to migrate by day 4. A confluent monolayer of lens epithelia cells was present on the posterior capsule at day 20. Total MMP-2 and -9 protein production increased with time with maximum levels reached on day 30. Conclusion MMP-2 and -9 were showed to be upregulated following sham cataract surgery. MMP expression could play an important role in PCO.

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Matrix metalloproteinase secretion is stimulated by TGF-ß in cultured lens epithelial cells

Abstract: These results indicate that TGF-beta-mediated processes could be responsible for localized lens capsular heterogeneity, establishing a substrate suitable for cell migration or the release of matrix-bound factors which influence the terminal differentiation of lens cells.

Cited by 26 publications

References 24 publications

Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells

Overexpression of matrix metalloproteinase-2 mediates phenotypic transformation of lens epithelial cells

Matrix metalloproteinases as mediators of primary and secondary cataracts

Expression of matrix metalloproteinases of human lens epithelial cells in the cultured lens capsule bag

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