Matrix Effect Elimination During LC–MS/MS Bioanalytical Method Development

Abstract

Due to the presence of endogenous components in biofluids, ionization suppression or enhancement may occur for bioanalytical assays using LC-MS or LC-MS/MS technologies. The matrix effect may affect the precision and accuracy of a bioanalytical method and, therefore, compromise the quality of the results. Protein precipitation sample preparation along with LC-MS/MS is a high-throughput method most commonly used in bioanalysis and is largely affected by the matrix effect. In order to eliminate the matrix effect during the method development, some considerations may be used: cleaner sample preparations, more sensitive instruments, which allow less material to be injected, different chromatographic separations and much more must be investigated. More than giving tools to adequately assess the matrix effect during the method development, this review gives scientists numerous ways to eliminate or reduce the matrix effect based on novel sample-preparation techniques, new chromatographic optimization methods and new technologies.

Comparative studies of HPLC-fluorometry and LC/MS method for the determination of N-acetylneuraminic acid as a marker of deteriorated ophthalmic solutions

“…These values corresponded to 73% and 68% of the added amount of NeuAc. From these data, LC/MS of NeuAc in hydrolyzed products show lower values than the predicted ones probably due to matrix effects [17,18]. The objective of the present study is to confirm the presence of NeuAc due to eye mucus materials in tear fluids, and to estimate the reason for the deterioration of ophthalmic solutions.…”

“…To quantify the amount of NeuAc, further studies (sample extraction, improvement chromatographic separation, use of the internal standard) are required to remove or minimize matrix effects [17,18].…”

Systematic evaluation of supported liquid extraction in reducing matrix effect and improving extraction efficiency in LC–MS/MS based bioanalysis for 10 model pharmaceutical compounds

“…Two techniques were used to monitor phospholipids. The first method targeted specific MRM transitions of major phospholipids [12,13,27] from different classes as shown in Table 2. These individual MRM transitions were selected based on their abundance in plasma and signal strength in mass spectrometer under current experimental conditions.…”

Validated UPLC/Q-TOF-MS Method for Determination of Poliumoside in Rat Plasma and Its Application to Pharmacokinetic Study

“…Mass spectrometry was performed on the Xevo G2 Q-TOF MS (Waters) equipped with the electronic spray ion (ESI) [13] under negative ion mode. The extraction cone, sampling cone and capillary voltages were 4, 40 and 3000 V, respectively.…”