Systematic evaluation of supported liquid extraction in reducing matrix effect and improving extraction efficiency in LC–MS/MS based bioanalysis for 10 model pharmaceutical compounds

LC–MS/MS method for simultaneous determination of thalidomide, lenalidomide, cyclophosphamide, bortezomib, dexamethasone and adriamycin in serum of multiple myeloma patients

“…In most cases, it was acceptable that recovery should be between 85% and 115% with a CV of < 15% [26]. However, as recovery can be substantially affected by the complex matrix, over 75% recovery was considered to be satisfied for compounds with diverse physicochemical properties [27]. The matrix effect of the analytes was determined by comparing the mean peak areas of the analytes in 3 replicates of post-extraction samples with those corresponding standards prepared by mobile phase.…”

Validating the use of an ultra-performance liquid chromatography with tandem mass spectrometry method to quantify equol in cow’s milk

“…The hydrolyzed sample was then centrifuged and extracted using n-hexane in order to carefully remove lipids from the milk (Stalikas 2007). Fat is one of the major causes of the matrix effect in LC-MS analysis (Jiang et al 2012). The second solvent was then used to extract equol (Stalikas 2007).…”

Recent developments in the chromatographic bioanalysis of approved kinase inhibitor drugs in oncology

“…Most article state that LLE provides clean extracts, but lipids tend to be co-extracted with the compound of interest. LLE sample preparation can also be time-consuming and hard to (fully-) automate, especially when compared to PP [86]. For LLE the solvents are mostly immiscible with water, requiring the removal of the solvents for compatibility with a reversed-phase chromatographic method.…”

“…In SLE, the same aqueous phases used in LLE are coated onto an inert diatomaceous earth support, but instead of shaking the two immiscible phases together, the organic phase is passed through the column and a very efficiently extraction takes place. SLE offers many advantages over LLE, including equivalent or more efficient extraction, no emulsion formation, easy automation, less organic solvent use, less labor, and less glassware [86]. The introduction of a 96-well format with a robotic liquid handling system for the analysis of erlotinib significantly improved the throughput of SLE.…”