Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors

Yssouf, Amina; Flaudrops, Christophe; Drali, Rezak; Kernif, Tahar; Socolovschi, Cristina; Bérenger, Jean-Michel; Raoult, Didier; Parola, Philippe

doi:10.1128/jcm.02665-12

Cited by 121 publications

(141 citation statements)

References 32 publications

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“…Most tests of nonmorphological methods used for the identification of some parasitic arthropods always used the blind approach, comparing the results of a candidate method against the background of morphological identification by specialists (i.e. Dieme et al, 2014;Yssouf et al, 2013). Examples of this approach to ticks so far cover the use of DNA-barcoding for the detection of the blood meal source (Gariepy et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The availability of different methods has provided insights in the use of cuticular hydrocarbon composition (Estrada-Peña et al, 1996) or MALDI-TOF (matrix assisted laser desorption ionizationtime of flight mass spectrometry) (Yssouf et al, 2013) for the identification of ticks, while most reports focused on the use of adequate genetic markers, like 16S rRNA, 12S rRNA, or cytochrome c oxidase I (coxI). Although these technologies are useful, they will always rely on reference specimens for which morphological identification needs to be correctly conducted (Nava et al, 2009;Araya-Anchetta et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

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A comparative test of ixodid tick identification by a network of European researchers

Estrada-Peña¹,

D’Amico

Palomar

et al. 2017

Ticks and Tick-borne Diseases

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This study reports the results of a comparative test of identification of ticks occurring in Western Europe and Northern Africa. A total of 14 laboratories were voluntarily enrolled in the test. Each participant received between 22 and 25 specimens of adult and nymphal ticks of 11 species: Dermacentor marginatus, D. reticulatus, Haemaphysalis punctata, Hyalomma lusitanicum, Hy. marginatum, Ixodes ricinus, I. hexagonus, Rhipicephalus annulatus, R. bursa, R. rossicus, and/or R. sanguineus s.l. Ticks were morphologically identified by three of the co-authors and the identification confirmed by a fourth co-author who used molecular methods based on several genes. Then ticks were randomly selected and blindly distributed among participants, together with a questionnaire. Only specimens collected while questing and, if possible, in the same survey, were circulated. Because of the random nature of the test, a participant could receive several specimens of the same species. Species in the different genera had variable misidentification rates (MR) of 7% (Dermacentor), 14% (Ixodes), 19% (Haemaphysalis), 36% (Hyalomma), and 54% (Rhipicephalus). Within genera, the MR was also variable ranging from 5.4% for I. ricinus or 7.4% for D. marginatus or D. reticulatus to 100% for R. rossicus. The test provided a total misidentification rate of 29.6% of the species of ticks. There are no significant differences in MR according to the sex of the tick. Participants were requested to perform a second round of identifications on the same set of ticks, using only purposely prepared keys (without illustrations), circulated to the enrolled participants, including 2 species of the genus Dermacentor, 8 of Haemaphysalis, 10 of Hyalomma, 23 of Ixodes, and 6 of Rhipicephalus. The average MR in the second round was 28%: 0% (Dermacentor), 33% (Haemaphysalis), 30% (Hyalomma) 18% (Ixodes), and 50% (Rhipicephalus). Species which are not reported in the countries of a participating laboratory had always highest MR, i.e. purely Mediterranean species had highest MR by laboratories in Central and Northern Europe. Participants expressed their concerns about a correct identification for almost 50% of the ticks of the genera Hyalomma and Rhipicephalus. The results revealed less than total confidence in identifying the most prominent species of ticks in the Western Palearctic, and underpin the need for reference libraries for specialists involved in this task. Results also showed that a combination of certain genes may adequately identify the target species of ticks. (Résumé d'auteur

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A comparative test of ixodid tick identification by a network of European researchers

Estrada-Peña¹,

D’Amico

Palomar

et al. 2017

Ticks and Tick-borne Diseases

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“…5,18,[31][32][33][34][35][36][37][38] Moreover, MALDI-TOF MS has enabled the rapid detection of tick and mosquito vectors without requiring previous expertise in entomology. 39,40 Thus, this technique will be of use in the implementation of effective prevention measures for vector-borne diseases. 39,40 In the future, it will be useful for tropical countries to evaluate whether MALDI-TOF MS may be used to distinguish between uninfected mosquitoes and those infected with sporozoites of Plasmodium spp.…”

Section: Discussionmentioning

confidence: 99%

“…39,40 Thus, this technique will be of use in the implementation of effective prevention measures for vector-borne diseases. 39,40 In the future, it will be useful for tropical countries to evaluate whether MALDI-TOF MS may be used to distinguish between uninfected mosquitoes and those infected with sporozoites of Plasmodium spp. and determine potential vector resistance to insecticides.…”

Section: Discussionmentioning

confidence: 99%

The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Fall¹,

Lo²,

Samb-Ba³

et al. 2015

The American Society of Tropical Medicine and Hygiene

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Abstract. Matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) represents a revolution in routine pathogen identification in clinical microbiology laboratories. A MALDI-TOF MS was introduced to tropical Africa in the clinical microbiology laboratory of the Hô pital Principal de Dakar (Senegal) and used for routine pathogen identification. Using MS, 2,429 bacteria and fungi isolated from patients were directly assayed, leading to the identification of 2,082 bacteria (85.7%) and 206 fungi (8.5%) at the species level, 109 bacteria (4.5%) at the genus level, and 16 bacteria (0.75%) at the family level. Sixteen isolates remained unidentified (0.75%). Escherichia coli was the most prevalent species (25.8%) followed by Klebsiella pneumoniae (14.8%), Streptococcus agalactiae (6.2%), Acinetobacter baumannii (6.1%), Pseudomonas aeruginosa (5.9%), and Staphylococcus aureus (5.9%). MALDI-TOF MS has also enabled the detection of rare bacteria and fungi. MALDI-TOF MS is a powerful tool for the identification of bacterial and fungal species involved in infectious diseases in tropical Africa.

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“…Additionally, this proteomic approach has been used for the identification of metazoans, such as fish species (33), plants (lentil varieties) (34), ticks (35,36), and insects, such as Drosophila fruit flies (37,38) and mosquitoes (39,40).…”

mentioning

confidence: 99%

Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

et al. 2015

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Biting midges of the genus Culicoides are implicated as vectors for a wide variety of pathogens. The morphological identification of these arthropods may be difficult because of a lack of detailed investigation of taxonomy for this species in Africa. However, matrix-assisted laser desorption ionization؊time of flight mass spectrometry (MALDI-TOF MS) profiling is efficient for arthropod identification at the species level. This study established a spectrum database of Culicoides spp. from Senegal using MALDI-TOF. Identification of Culicoides insects to the species level before mass spectrometry was performed on the basis of morphological characters. MALDI-TOF MS reference spectra were determined for 437 field-caught Culicoides of 10 species. The protein profiles of all tested Culicoides revealed several peaks with mass ranges of 2 to 20 kDa. In a validation study, 72 Culicoides specimens in the target species were correctly identified at the species level with a similarity of 95 to 99.9%. Four Culicoides protein profiles were misidentified. Nevertheless, six SuperSpectra (C. imicola, C. enderleini, C. oxystoma, C. kingi, C. magnus, and C. fulvithorax) were created. Abdomens of midges were used to amplify and sequence a portion of the mitochondrial cytochrome oxidase I gene (COI). The results obtained using the MALDI-TOF MS method were consistent with the morphological identification and similar to the genetic identification. Protein profiling using MALDI-TOF is an efficient approach for the identification of Culicoides spp., and it is economically advantageous for approaches that require detailed and quantitative information of vector species that are collected in field. The database of African Culicoides MS spectra created is the first database in Africa. The COI sequences of five Culicoides species that were previously noncharacterized using molecular methods were deposited in GenBank. Biting midges of the genus Culicoides are among the smallest hematophagous insects, and they can be a nuisance to humans and animals (1). These insects are implicated as vectors for a wide variety of viruses, such as bluetongue virus (2), African horse sickness virus (3), epizootic hemorrhagic disease virus (4), the Schmallenberg virus that recently emerged in Europe (5-7), Toggenburg orbivirus (8), and the Oropouche virus (9), which is the only human virus transmitted by Culicoides. Additionally, biting midges are involved in the transmission of various human filarial parasites, such as Mansonella perstans (10-13), Mansonella streptocerca, Mansonella ozzardi, and Mansonella rodhaini (14). Recently, Leishmania spp. and Leishmania infantum were detected in Culicoides imicola and Culicoides spp. (15).Vector identification is an important step in the epidemiology of vector-borne diseases. Information on the major vector species may provide a clearer indication of the geographic distribution of the disease or its potential distribution, the location of danger points for high risks of contact with the vector, and access to alternative...

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Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry for Rapid Identification of Tick Vectors

Cited by 121 publications

References 32 publications

A comparative test of ixodid tick identification by a network of European researchers

A comparative test of ixodid tick identification by a network of European researchers

The Ongoing Revolution of MALDI-TOF Mass Spectrometry for Microbiology Reaches Tropical Africa

Comparison of Matrix-Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry and Molecular Biology Techniques for Identification of Culicoides (Diptera: Ceratopogonidae) Biting Midges in Senegal

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