Matrix-assisted laser desorption/ionization detection of hemoglobin from long-stored samples of human blood

Smolira, A.; Ptasińska, Sylwia; Smolira, Marcin; Michalak, Leszek

doi:10.1016/j.vacuum.2005.01.102

Cited by 5 publications

(3 citation statements)

References 29 publications

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“…The presented investigations used a home‐built linear TOF mass spectrometer, which has already been described in detail elsewhere . Briefly, the instrument was operated in positive ion linear mode with an accelerating potential of +17 kV applied to the sample holder.…”

Section: Methodsmentioning

confidence: 99%

“…The presented investigations used a home-built linear TOF mass spectrometer, which has already been described in detail elsewhere. [23] Briefly, the instrument was operated in positive ion linear mode with an accelerating potential of +17 kV applied to the sample holder. The VSL-337ND-S 337 nm nitrogen laser (Laser Innovations, Santa Paula, CA, USA) has an output pulse length of 4 ns, an output beam size of approximately 35 mm 2 , a maximum pulse energy of 300 μJ, and a peak power of~75 kW.…”

Section: Maldi-tof Instrumentationmentioning

confidence: 99%

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Quantification of the PR‐39 cathelicidin compound in porcine blood by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Smolira

Hałas

Wessely-Szponder

2015

Rapid Comm Mass Spectrometry

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Section: Methodsmentioning

confidence: 99%

Section: Maldi-tof Instrumentationmentioning

confidence: 99%

Quantification of the PR‐39 cathelicidin compound in porcine blood by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Smolira

Hałas

Wessely-Szponder

2015

Rapid Comm Mass Spectrometry

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“…In the presented studies, the linear mode of the spectrometer was used. Detailed parameters of the apparatus have already been discussed elsewhere [ 62 ]. The molecules are ionized with the nitrogen laser (LNC300C; Laser Photonics, New York, NY, USA, λ = 337 nm, an output length of 5 ns, a maximum pulse energy specified as 250 μJ, and a rectangular shape of approximately 4 × 9 mm).…”

Section: Methodsmentioning

confidence: 99%

Importance of the Matrix and the Matrix/Sample Ratio in MALDI-TOF-MS Analysis of Cathelicidins Obtained from Porcine Neutrophils

Smolira

Wessely-Szponder

2014

Appl Biochem Biotechnol

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Qualitative and quantitative mass spectrometric studies of biomolecules for example proteins, peptides, or lipids contained in biological samples like physiologic fluids are very important for many fields of science such as medicine, veterinary medicine, biology, biochemistry, molecular biology, or environmental sciences. In the last two decades, MALDI TOF MS — matrix-assisted laser desorption mass spectrometry, proved to be an especially convenient tool for these analyses. The main advantages of this method are its rapidity and high sensitivity which is particularly appreciated in the case of studies of complex biological specimen. A major challenge for many researchers is to maximize this sensitivity, among others, by appropriate procedures of sample preparation for the measurement. The objective of this work was to optimize these procedures, selecting the optimal matrix and optimum proportions of the sample and the matrix solution in a mixture of both solutions, aiming at the achievement of the maximum intensity of ion current. In this respect, five low molecular mass cathelicidins were studied: prophenin-2, protegrins 1–3, PR-39. All of them were obtained directly from the porcine blood. As a result of studies, the authors determined such experimental conditions when the intensity of investigated ionic current had the highest value.

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Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for the detection of hemoglobins as the protein biomarkers for fecal occult blood

Lin

Shih

et al. 2007

Rapid Comm Mass Spectrometry

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In this study, we discover that hemoglobins (Hb), highly water-soluble globular proteins that are the most predominant proteins detected by matrix-assisted laser desorption ionization/time-of-flight (MALDI-TOF) mass spectrometry in blood, can be used as protein biomarkers for fecal occult blood (FOB). Hemoglobins were extracted from the feces with pure water and separated from the solids in feces through centrifugation. Singly charged molecular ions of Hb-related alpha chains (theoretical MW: 15 126) and beta chains (theoretical MW: 15 867) were detected by MALDI-TOF operated in linear mode using 4-hydroxy-alpha-cyanocinnamic acid (alpha-CHC) as the matrix (with a volumetric ratio of 1:1). The detection limit of FOB using this method is estimated to be lower than 0.1 microg blood per mg of feces, which is approximately 10 to 100 times lower than that of the conventional chemical approaches. The foods and dietary supplements that commonly interfere with the conventional chemical assays of FOB - such as animal blood food products and tablets containing iron and vitamin C - do not interfere with the detection of Hb biomarkers during MALDI-TOF analysis.

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Matrix-assisted laser desorption/ionization detection of hemoglobin from long-stored samples of human blood

Cited by 5 publications

References 29 publications

Quantification of the PR‐39 cathelicidin compound in porcine blood by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Quantification of the PR‐39 cathelicidin compound in porcine blood by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry

Importance of the Matrix and the Matrix/Sample Ratio in MALDI-TOF-MS Analysis of Cathelicidins Obtained from Porcine Neutrophils

Matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry for the detection of hemoglobins as the protein biomarkers for fecal occult blood

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