Matrine impedes colorectal cancer proliferation and migration by downregulating endoplasmic reticulum lipid raft associated protein 1 expression

Abstract

Matrine exhibits anti-tumor effect on the proliferation and invasion of colorectal cancer (CRC) cells by reducing the activity of the p38 signaling pathway. However, these studies were limited because the underlying mechanism by which matrine inhibited CRC progression remained unclear. In this study, we provided for the first time that endoplasmic reticulum lipid raft associated protein 1 (Erlin1) is a novel target of matrine. Erlin1 was significantly upregulated in tumors and its knockdown suppressed the proliferation and migration of CRC cells, while its overexpression promoted CRC cell growth and migration. Furthermore, Erlin1 overexpression promoted inhibited apoptosis. Importantly, matrine treatment could reverse the oncogenic function of Erlin1 on CRC cell proliferation and migration. When Erlin1 was knocked down, matrine exhibited a more obvious anti-tumor effect in CRC cells. Partly due to this, matrine functions as an important anti-tumor drug and the results discovered here may clarify the mechanisms of matrine application for CRC treatment. CRC patients with low expression of Erlin1 might be more suitable for the treatment of matrine. This study could promote the application of matrine to be a promising therapeutic strategy for CRC patients.

Matrine exerts an anti‐tumor effect via regulating HN1 in triple breast cancer both in vitro and in vivo

“…Matrine can promote the autophagy of cervical cancer cells through the Akt/mTOR signaling pathway and can be used as an autophagy inducer of cervical cancer (Zhang et al, 2022). Besides, matrine inhibits the proliferation and migration of colorectal cancer cells and promotes the apoptotic ability of cancer cells (Ren et al, 2022). In addition, matrine can inhibit the proliferation and migration of breast cancer cells and promote the apoptosis of cancer cells by inhibiting the expression of vascular endothelial factors (Xiao et al, 2018).…”

Matrine restrains the development of colorectal cancer through regulating the AGRN/Wnt/β‐catenin pathway

“…Additionally, it has been revealed the anti‐cancer action of matrine in colorectal cancer, for instance, matrine regulated Bcl‐2 and caspase‐3 expression to boost cell apoptosis in colorectal cancer cells with minimum side effects 29 . Moreover, matrine downregulated Erlin1 experssion to impede the metastasis and growth of colorectal cancer cells 30 . However, the anti‐tumor role of matrine and its underlying mechanisms in colorectal cancer need largely exploration.…”

“…29 Moreover, matrine downregulated Erlin1 experssion to impede the metastasis and growth of colorectal cancer cells. 30 However, the anti-tumor role of matrine and its underlying mechanisms in colorectal cancer need largely exploration.…”

Research Advancements in Pharmacological Activities and Mechanisms of Matrine

“…Matrine is a novel approach to treating CRC, and its mode of action is to decrease CRC cell proliferation and invasion by downregulating p38 and microRNA-22 expression (Liu J, et al, 2020; Ren et al, 2014). Matrine, an ER membrane-associated protein with an N-terminal transmembrane structural domain, may inhibit the G1 phase of the cell cycle and cause apoptosis in CRC cells by downregulating the Erlin1 overexpression (Ren et al, 2022). High concentrations of matrine have a significant toxic effect on CRC cells.…”