Matrine alleviates lipopolysaccharide-induced intestinal inflammation and oxidative stress via CCR7 signal

Abstract

The aim of this study was to investigate the protective effects of matrine on lipopolysaccharide (LPS)-induced inflammation and oxidative stress in vivo and in vitro. The results showed that matrine improved intestinal inflammatory status and oxidative balance and enhanced chemokine receptor 7 (CCR7) expression. In LPS-challenged mice and Caco-2 cells, matrine alleviated LPS-induced inflammation and oxidative stress via downregulating pro-inflammatory cytokines (IL-1β and IL-17) and malondialdehyde (MDA) production. CCR7-siRNA transfection blocked the protective effects of matrine on LPS-induced inflammation and oxidative stress and exacerbated LPS caused injury. In conclusion, matrine alleviates LPS-induced intestinal inflammation and oxidative stress in mice and Caco-2 cells, which may be associated with CCR7 signal.

Matrine reduces cigarette smoke-induced airway neutrophilic inflammation by enhancing neutrophil apoptosis

“…Thus, resolution of inflammation is key to treat COPD. In the present study, we tested the hypothesis that matrine could suppress inflammation in the airway of mice exposed to CS based on its anti-inflammatory effect in other inflammatory diseases [29,37]. In our in vivo and ex vivo studies, we found that matrine dampened airway inflammation by reducing the influx of neutrophils but not macrophages in the airways of CS-exposed mice.…”

“…Matrine and its different derivatives were reported to have multiple effects in different disease models. For example, matrine can prevent bone loss [38], protect glial cells from central nervous system inflammation [39], alleviate intestinal inflammation [29], and decrease liver cell injury [40]. Additionally, it is reported that matrine showed very strong anti-tumor effect which includes cytotoxic effect on prostate tumor cells, bladder tumor cells, and breast cancer cells [31,41,42].…”

“…Matrine is one of the active components of Sophora flavescens Ait (Kushen) which is currently being used to treat liver viral infection and tumors in China [26,27]. In previous studies, matrine has shown anti-inflammatory and antioxidative effects in acute lung injury and intestinal inflammation induced by LPS [28,29]. A study by Zeng et al [30] showed that matrine alleviated the inflammation in a mouse model of insulin resistance and hepatic steatosis by increasing the hepatic levels of heat shock protein HSP72.…”

Matrine Is Identified as a Novel Macropinocytosis Inducer by a Network Target Approach

“…In fact, the biological activities of matrine are more relevant to other applications of Ku-Shen other than anti-tumor activities. It is reported to possess a variety of pharmacological activities, including antipyretic ( Cho et al, 1986 ; Xiang and Jiang, 2013 ), antiepileptic ( Xiang and Jiang, 2013 ), antinociceptive ( Wang et al, 2013 ; Dun et al, 2014 ), anti-inflammatory ( Zhang B. et al, 2011 ; Wu G. et al, 2017 ), myocardial preservation ( Zhang et al, 2006 ), antifibrotic ( Zhang et al, 2001 ), anti-tumor ( Wu J. et al, 2017 ), anti-viral ( Li et al, 2005 ) and anti-angiogenic ( Li et al, 2010 ). Our predicted results demonstrated that matrine could target a molecular network related to multiple functions, which is consistent with the multi-target nature of traditional medicine.…”

“…Recently, a proven group of Chinese patent medicines has held and still holds an important position in the treatment of a variety of cancers in China, and statistical analysis results from nationwide medical insurance data have suggested that Sophorae Flavescentis Radix , namely Ku-Shen , is widely used in the treatment of a variety of cancers in China ( Wu et al, 2015 ). Matrine is a main active constituent in Ku-Shen which has been approved as herbal medicine in China, and has polypharmacology including anti-inflammatory ( Zhang B. et al, 2011 ; Wu G. et al, 2017 ), anti-tumor ( Wu J. et al, 2017 ), anti-angiogenic ( Li et al, 2010 ) and so on. It is a kind of quinolizidine alkaloid compound and very similar in structure to oxymatrine and sophoridine.…”