Mating system of a population of Myracrodruon urundeuva F.F. & M.F. Allemão using the fAFLP molecular marker

Abstract

The mating system and genetic diversity were studied in a natural population of Myracrodruon urundeuva originating from 30 open-pollinated trees at the Paulo de Faria Ecological Station, SP, Brazil. The progenies were planted on the Teaching and Research Farm of the Ilha Solteira Engineering School, UNESP. Using the fAFLP molecular marker, eleven loci were selected to study the mating system. The mating system was analyzed using the multilocus mixed-mating model. The estimates of genetic divergence between pollen and ovule allele frequencies were significant for eight loci, suggesting nonrandom outcrossing. The estimates of the multilocus outcrossing rate revealed that M. urundeuva possesses a mating system with a predominance of outcrossing events ( $ θ = 0.940 ± 0.086). The estimates of coancestry among plants within progenies ( $ θ = 0.185) was higher than that expected for half-sib progenies (0.125) and the indirect estimate of the correlation of outcrossed paternity within progeny arrays ( $ ) r p was 0.403, suggesting that progenies have a high proportion of full-sibs. Result analysis suggests the need for the application of biometric models that take into account deviations from random outcrossing in the estimations of genetic parameters for quantitative traits and the need for retaining large sample sizes in order to preserve genetic variability.

Outcrossing rate between 'Haden' and 'Tommy Atkins' mangoes estimated using microsatellite and AFLP markers

“…The outcrossing estimates obtained in the present study and by Degani et al (1997) indicate that mango is a predominantly open pollinated species and that biometrical models applied to mangoes should take into account the deviation from random outcrossing to estimate genetic parameters and the constitution of broad germplasm samples to preserve the genetic variability of the species, as recommended by Freitas et al (2004), while evaluating Myracrodruon urundeuva.…”

“…Therefore, it is important to establish strategies for the effective conservation of any species. The mating system, together with the mechanism of pollen and seed dispersion, determines part of the genetic structure of populations (Freitas et al, 2004).…”

Estimation of Mating System in the Endangered Aquatic Fern Ceratopteris pteridoides in China Based on AFLP Molecular Marker and Selfing Test: Implications for Conservation

“…In recent studies, outcrossing rates have been traditionally studied using co-dominant markers such as isozymes and microsatellites due to their high allelic polymorphism (Chaix et al, 2003;Adugna et al, 2013;Sinha et al, 2015;Sharma et al, 2017). However, when microsatellite markers were unavailable, several dominant markers such as RAPD (Gjuric and Smith, 1996), ISSR (Ge and Sun, 1999;Han et al, 2009), and AFLP have been used to estimate outcrossing rates as they are able to produce very large numbers of informative loci without previous genetic information about the genetic system in plant species (Gaiotto et al, 1997;Freitas et al, 2004;Muluvi et al, 2004;Pometti et al, 2013;Sinha et al, 2015;Sharma et al, 2017 ). Amplified fragment length polymorphisms (AFLPs) are easily applied to new species, since DNA sequence knowledge is not required for this technique unlike co-dominant markers such as microsatellites (Vos et al, 1995).…”

An AFLP estimation of the outcrossing rate of <i>Spondias tuberosa</i> (Anacardiaceae), an endemic species to the Brazilian semiarid region

“…A species can produce offspring through different types of mating, such as random outcrossing, correlated outcrossing, biparental inbreeding, self-pollination, apomixes or a combination of these reproductive strategies. Plant reproduction, together with the mechanisms of pollen and seed dispersal, determines the genetic structure of plant populations (Freitas et al 2004).The plant species that result from outcrossing maintain the majority of their distributed genetic variability within the population, in contrast to a predominantly self-pollinated species in which the majority of the genetic variability is distributed between populations (Hamrick & Godt 1989). Reproductive studies have revealed that a large number of plant species are allogamous or possess a mixed mating system that is predominantly allogamous (Gusson et al 2006).…”

“…Therefore, biometrical models applied to this species should take into account deviation from random outcrossing to estimate genetic parameters. As recommended by Freitas et al (2004), while evaluating Myracrodruon urundeuva, biometrical models should also account for the constitution of broad germplasm samples to preserve the genetic variability of the species.The high heterozygosity levels observed in the analyzed umbu tree families were also an important variable for the in situ conservation of this species because new allelic recombination can occur, thus making it possible for the appearance of new genotypes that are readily adapted to environmental changes and for the colonization of new areas.More than 932 savanna-like plant species, including 380 endemic species, have been identified in the Brazilian semiarid Region (Brasil 2002). The majority of these species have not been studied or sampled to form a germplasm collection, which increases the risk of losing genetic variability of the adapted species to xerophytic conditions.…”