Mathematical descriptive characteristics of potato tubers' shape

Bubeníčková, Aneta; Simeonovová, Jana; Kumbár, Vojtěch; Jůzl, Miroslav; Nedomová, Šárka

doi:10.11118/actaun201159060063

Cited by 2 publications

(2 citation statements)

References 17 publications

(20 reference statements)

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“…To obtain nodule volume, we extracted and measured the area and perimeter of the nodules photographed using FIJI (Schindelin et al, 2012). We calculated the corresponding prolate spheroid volume using the best fitted ellipse of each nodule based on a previously developed formula (Nedomová et al, 2014). We weighed nodules and kept them at 4°C in 0.9% NaCl solution until they could be crushed for fitness assays.…”

Section: Harvestmentioning

confidence: 99%

The Non-Legume Parasponia andersonii Mediates the Fitness of Nitrogen-Fixing Rhizobial Symbionts Under High Nitrogen Conditions

2020

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Organisms rely on symbiotic associations for metabolism, protection, and energy. However, these intimate partnerships can be vulnerable to exploitation. What prevents microbial mutualists from parasitizing their hosts? In legumes, there is evidence that hosts have evolved sophisticated mechanisms to manage their symbiotic rhizobia, but the generality and evolutionary origins of these control mechanisms are under debate. Here, we focused on the symbiosis between Parasponia hosts and N 2-fixing rhizobium bacteria. Parasponia is the only non-legume lineage to have evolved a rhizobial symbiosis and thus provides an evolutionary replicate to test how rhizobial exploitation is controlled. A key question is whether Parasponia hosts can prevent colonization of rhizobia under high nitrogen conditions, when the contribution of the symbiont becomes nonessential. We grew Parasponia andersonii inoculated with Bradyrhizobium elkanii under four ammonium nitrate concentrations in a controlled growth chamber. We measured shoot and root dry weight, nodule number, nodule fresh weight, nodule volume. To quantify viable rhizobial populations in planta, we crushed nodules and determined colony forming units (CFU), as a rhizobia fitness proxy. We show that, like legumes and actinorhizal plants, P. andersonii is able to control nodule symbiosis in response to exogenous nitrogen. While the relative host growth benefits of inoculation decreased with nitrogen fertilization, our highest ammonium nitrate concentration (3.75 mM) was sufficient to prevent nodule formation on inoculated roots. Rhizobial populations were highest in nitrogen free medium. While we do not yet know the mechanism, our results suggest that control mechanisms over rhizobia are not exclusive to the legume clade.

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Section: Harvestmentioning

confidence: 99%

The Non-Legume Parasponia andersonii Mediates the Fitness of Nitrogen-Fixing Rhizobial Symbionts Under High Nitrogen Conditions

2020

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“…To measure nodule volume, the area and perimeter of each nodule were extracted from these images using FIJI (Schindelin et al, 2012). The corresponding prolate spheroid volume of each nodule was measured by using the best-fitted ellipse to calculate this volume based on a previously developed formula (Bubeníčková et al, 2014).…”

Section: Bacteria Inoculationmentioning

confidence: 99%

Conserved mechanisms in nodulation and arbuscular mycorrhization in the non-legume Parasponia andersoniiy

Roswanjaya

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Parasponia species (Cannabaceae) are the only known non-legumes able to establish a nitrogen-fixing nodule symbiosis with rhizobium. Also, Parasponia species can interact with arbuscular mycorrhizal fungi (AMF), which enhances uptake of nutrients, especially phosphates. The interaction between plants and AMF is generally hard to quantify because the success of this endosymbiosis depends on the condition of both partners as well as environmental factors. Endomycorrhization assays in Parasponia still rely on methods as established for model plant species. However, those methods could mask specific phenotypes because each plant species may require specific conditions to develop an efficient AMF symbiosis. Here, we did serial methodological optimizations to determine optimal conditions for the interaction between Parasponia andersonii and the AMF Rhizophagus irregularis. By quantifying four parameters of mycorrhization efficiency, we show that in two concentrations of exogenous phosphate (20 µM and 40 µM), the interaction between P. andersonii and R. irregularis occurred very efficiently. In addition, the use of a close pot system to avoid any contamination during the assay did not affect mycorrhization efficiency nor plant growth parameters. Furthermore, quantification of mycorrhization efficiency from two different plant materials -seedling and tissue culture plantlets-revealed that vegetative propagated P.andersonii requires a double amount of spores compared to generatively propagated plantlets to obtain a similar level of AMF colonization. Taken together, the newly developed assay will allow quantitative phenotyping of the AMF symbiosis in P. andersonii mutants.

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Mathematical descriptive characteristics of potato tubers' shape

Cited by 2 publications

References 17 publications

The Non-Legume Parasponia andersonii Mediates the Fitness of Nitrogen-Fixing Rhizobial Symbionts Under High Nitrogen Conditions

The Non-Legume Parasponia andersonii Mediates the Fitness of Nitrogen-Fixing Rhizobial Symbionts Under High Nitrogen Conditions

Conserved mechanisms in nodulation and arbuscular mycorrhization in the non-legume Parasponia andersoniiy

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