“…A wide range of birth defects, the formation of which occurs in the early stages of cardiogenesis is evidence of the vulnerability of the embryonic heart during this period. This necessitates the use of a significant arsenal of research methods: light microscopy (Ream et al, 2008), immunohistochemistry (Garlanda et al, 1997Azar et al, 2003;Dong & Thompson, 2006;Tonne et al, 2011), electron microscopy (Schaper et al, 1985;McKenzie et al, 1994;Zhang & Pasumarthi, 2007;Oxford et al, 2011;Cury et al, 2012), microcomputer tomography (Nieman & Turnbull, 2010;Gilbert et al, 2012), ultrasound microscopy (Araujo Júnior et al, 2012;Hernandez-Andrade et al, 2012;Hongmei et al, 2012;Gindes et al, 2012), echocardiography (Choi et al, 2013Lee &Won, 2013), confocal microscopy (Sands et al, 2005), and three-dimensional visualization (Momoi et al, 2012). Each method has its advantages and disadvantages.…”