Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize

Kelliher, Timothy; Starr, Dakota; Wang, Wenling; McCuiston, Jamie; Zhong, Heng; Nuccio, Michael L.; Martin, Bruce D.

doi:10.3389/fpls.2016.00414

Cited by 114 publications

(87 citation statements)

References 20 publications

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“…MiMe combined with genome elimination indeed leads to clonal offspring, as previously demonstrated in Arabidopsis [13]. However, the CENH3 manipulation used in Arabidopsis to provoke genome elimination has not been transferred in other species to date [20,[39][40][41], except in maize but with a lower frequency [42]. The frequency of genome elimination can be genetically controlled in cereals, notably in maize [43][44][45].…”

Section: Discussionmentioning

confidence: 63%

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Turning rice meiosis into mitosis

Mieulet¹,

Jolivet²,

Rivard³

et al. 2016

Cell Res

114

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Introduction of clonal reproduction through seeds (apomixis) in crops has the potential to revolutionize agriculture by allowing self-propagation of any elite variety, in particular F1 hybrids. In the sexual model plant Arabidopsis thaliana synthetic clonal reproduction through seeds can be artificially implemented by (i) combining three mutations to turn meiosis into mitosis (MiMe) and (ii) crossing the obtained clonal gametes with a line expressing modified CENH3 and whose genome is eliminated in the zygote. Here we show that additional combinations of mutations can turn Arabidopsis meiosis into mitosis and that a combination of three mutations in rice (Oryza sativa) efficiently turns meiosis into mitosis, leading to the production of male and female clonal diploid gametes in this major crop. Successful implementation of the MiMe technology in the phylogenetically distant eudicot Arabidopsis and monocot rice opens doors for its application to any flowering plant and paves the way for introducing apomixis in crop species.

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Section: Discussionmentioning

confidence: 63%

“…The caryopses were then washed thrice in sterile distilled water. Embryos were isolated following two distinct procedures depending on the presence or absence of endosperm [42,43]. Immature embryos were plated on MS basal medium [44] supplemented with 50 g/l sucrose and 6 g/l phytagel.…”

Section: Embryo Rescuementioning

confidence: 99%

Turning rice meiosis into mitosis

Mieulet¹,

Jolivet²,

Rivard³

et al. 2016

Cell Res

114

View full text Add to dashboard Cite

show abstract

“…This approach has particular appeal as CENH3 is a conserved protein across all plant species, meaning it can potentially be applied to generate haploid inducers in any plant species. This was recently demonstrated in maize and barley, although in barley, multiple copies of CENH3 complicated the haploid induction (Karimi-Ashtiyani et al 2015; Kelliher et al 2016). …”

Section: Why Study Apomixis?mentioning

confidence: 89%

Seeds of doubt: Mendel’s choice of Hieracium to study inheritance, a case of right plant, wrong trait

et al. 2016

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Key message In this review, we explore Gregor Mendel’s hybridization experiments with Hieracium , update current knowledge on apomictic reproduction and describe approaches now being used to develop true-breeding hybrid crops. AbstractFrom our perspective, it is easy to conclude that Gregor Mendel’s work on pea was insightful, but his peers clearly did not regard it as being either very convincing or of much importance. One apparent criticism was that his findings only applied to pea. We know from a letter he wrote to Carl von Nägeli, a leading botanist, that he believed he needed to “verify, with other plants, the results obtained with Pisum”. For this purpose, Mendel adopted Hieracium subgenus Pilosella, a phenotypically diverse taxon under botanical study at the time. What Mendel could not have known, however, is that the majority of these plants are not sexual plants like pea, but instead are facultatively apomictic. In these forms, the majority of seed arises asexually, and such progeny are, therefore, clones of the maternal parent. Mendel obtained very few hybrids in his Hieracium crosses, yet we calculate that he probably emasculated in excess of 5000 Hieracium florets to even obtain the numbers he did. Despite that effort, he was perplexed by the results, and they ultimately led him to conclude that “the hybrids of Hieracium show a behaviour exactly opposite to those of Pisum”. Apomixis is now a topic of intense research interest, and in an ironic twist of history, Hieracium subgenus Pilosella has been developed as a molecular model to study this trait. In this paper, we explore further Mendel’s hybridization experiments with Hieracium, update current knowledge on apomictic reproduction and describe approaches now being used to develop true-breeding hybrid crops.

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“…Recently, Kelliher et al (2016) have reported CENH3-mediated haploid production in maize. In this study, native CENH3 was silenced using RNAi approach or by transposon insertion.…”

Section: Significance Of Cenh3 In Plant Breeding a Novel Methods Of Hamentioning

confidence: 99%

Centromeric histone H3 protein: from basic study to plant breeding applications

Watts

Kumar

Bhat

2016

J. Plant Biochem. Biotechnol.

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Centromere is the defining unit of a chromosome where kinetochore complex assembles and facilitates chromosome segregation. Centromeres contain unique repetitive sequences and are enriched with transposons and retrotransposons. Although how centromere is determined is still not clearly understood, binding of a key protein, namely, the Centromeric Histone H3 (CENH3) to centromeric repetitive DNA sequences has been found to be critical for the specification of centromere. Hence, centromeres are said to be epigenetically specified by CENH3. Despite considerable variation in size and sequence, CENH3 protein shows significant conservation of structure and function. CENH3 disruption or overexpression shows severe defects in spindle fiber attachment and ultimately leads to embryo lethality. Basic studies on complementation of CENH3 in Arabidopsis thaliana have led to the development of a novel method of haploid production through selective elimination of one set of parental chromosomes in the zygote. These findings have also shed new light on selective loss of chromosomes in interspecific crosses of Hordeum vulgare 9 H. bulbosum. Here, we briefly review unique features of CENH3 and discuss the new plant breeding opportunities that have emerged from the study of CENH3.

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Maternal Haploids Are Preferentially Induced by CENH3-tailswap Transgenic Complementation in Maize

Cited by 114 publications

References 20 publications

Turning rice meiosis into mitosis

Turning rice meiosis into mitosis

Seeds of doubt: Mendel’s choice of Hieracium to study inheritance, a case of right plant, wrong trait

Centromeric histone H3 protein: from basic study to plant breeding applications

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