Maternal glyphosate-based herbicide exposure alters antioxidant-related genes in the brain and serum metabolites of male rat offspring

Souza, Janaína Sena de; Laureano‐Melo, Roberto; Herai, Roberto Hirochi; Conceição, Rodrigo Rodrigues da; Oliveira, Kelen Carneiro; Silva, Ismael Dale Cotrim Guerreiro da; Silva, Magnus R. Dias da; Romano, Renata Marino; Romano, Marco Aurélio; Maciel, Rui M. B.; Chiamolera, Maria Izabel; Giannocco, Gisele

doi:10.1016/j.neuro.2019.06.004

Cited by 33 publications

(18 citation statements)

References 76 publications

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“…Another mouse model also showed that inhibition or deletion of neuronal HIF1α increased necrotic and apoptotic cell death [ 56 ]. After exposing pregnant rats to glyphosate-based herbicides, one study found offspring to exhibit significant increases (73%, p = 0.017) in HIF1α in the cerebellum [ 57 ]. Following pesticide exposure, the placentas of a cohort of pregnant tea garden workers showed increased levels of HIF1α expression [ 58 ].…”

Section: Discussionmentioning

confidence: 99%

In Silico Exploration of the Potential Role of Acetaminophen and Pesticides in the Etiology of Autism Spectrum Disorder

Furnary

García-Milián

Liew

et al. 2021

Toxics

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Recent epidemiological studies suggest that prenatal exposure to acetaminophen (APAP) is associated with increased risk of Autism Spectrum Disorder (ASD), a neurodevelopmental disorder affecting 1 in 59 children in the US. Maternal and prenatal exposure to pesticides from food and environmental sources have also been implicated to affect fetal neurodevelopment. However, the underlying mechanisms for ASD are so far unknown, likely with complex and multifactorial etiology. The aim of this study was to explore the potential effects of APAP and pesticide exposure on development with regards to the etiology of ASD by highlighting common genes and biological pathways. Genes associated with APAP, pesticides, and ASD through human research were retrieved from molecular and biomedical literature databases. The interaction network of overlapping genetic associations was subjected to network topology analysis and functional annotation of the resulting clusters. These genes were over-represented in pathways and biological processes (FDR p < 0.05) related to apoptosis, metabolism of reactive oxygen species (ROS), and carbohydrate metabolism. Since these three biological processes are frequently implicated in ASD, our findings support the hypothesis that cell death processes and specific metabolic pathways, both of which appear to be targeted by APAP and pesticide exposure, may be involved in the etiology of ASD. This novel exposures-gene-disease database mining might inspire future work on understanding the biological underpinnings of various ASD risk factors.

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Section: Discussionmentioning

confidence: 99%

In Silico Exploration of the Potential Role of Acetaminophen and Pesticides in the Etiology of Autism Spectrum Disorder

Furnary

García-Milián

Liew

et al. 2021

Toxics

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“…Recently, our group demonstrated the interference of GBH in the TH axis of perinatal exposed animals 27 . Additionally, we showed that antioxidant‐related genes are affected in the cerebellum of the GBH‐treated animals, that significantly affected the concentration of metabolites in the serum, 28 suggesting that maternal GBH‐exposure also affects the offspring in adulthood, not only restricted to hormonal level but also in the normal function of cerebellum.…”

Section: Introductionmentioning

confidence: 88%

“…The metabolic data were collected from blood samples of the treated animals as described here. This same dataset was previously analyzed by our group in another study 28 that made it available for this work. Here, the raw metabolic data was subjected to an improved analysis using updated databanks and analytical approaches to improve the statistical analysis.…”

Section: Methodsmentioning

confidence: 99%

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Intergenerational thyroid hormone homeostasis imbalance in cerebellum of rats perinatally exposed to glyphosate‐based herbicide

Reis

Souza

Herai

et al. 2021

Environmental Toxicology

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Agrochemicals became a public health concern due to increased human exposure and possible endocrine disruption effects in several organs, including the brain. Thyroid hormones controls neurodevelopment, which turn them sensitive to endocrine disruptors (EDs). In this work, we evaluated the effect of glyphosate-based herbicides (GBH) as an intergenerational endocrine disrupter on thyroid homeostasis in cerebellar cells. Female pregnant Wistar rats were exposed to Roundup Transorb ® solution at 5 and 50 mg/kg/day, from gestation day 18 to post-natal day 5 (P5). Cerebellum of male offspring was used to evaluate gene expression. The mRNA levels of thyroid hormone receptors, hormonal conversion enzymes, hormone transporters, as well as, de novo epigenetic regulators were altered, with some of these genes presenting a non-monotonic dose response. Furthermore, metabolomic profile correlation with tested dose demonstrated altered metabolic profile, in agreement with cerebellar gene alterations. Moreover, cerebellar primary cultures exposed to non-toxic GBH concentration presented a decrease level in glial fibrillary acidic protein, a protein regulated by endocrine signals. In conclusion, our results indicate that animals exposed to non-toxic GBH doses during perinatal phase carry intergenerational alterations in key regulators of cellular thyroid hormone homeostasis and epigenetic controllers in adulthood, indicating the possible ED effect of GBH based on epigenetic alterations.

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“…ROS düzeyi arttığında, antioksidan kapasite azaldığında canlı sistemi oluşturan biyomoleküllerde oksidatif hasar oluşmakta ve hücre ölümü gerçekleşmektedir. Ortaya çıkan bilgiler, herbisitlerin canlı sistemde ROS düzeyinin artmasını teşvik ederek canlı sistemde patolojik koşulların gelişimine zemin hazırladığını göstermektedir [19,20]. Glifosatın, canlı sistemin antioksidan aktivitesini bozduğu ve oksidatif stres parametrelerinde artışa neden olduğu tespit edilmiştir [20,21].…”

Section: Sonuçlar Ve Tartışmaunclassified

Effect of vanillic acid against oxidative stress induced by glyphosate in Saccharomyces cerevisiae

Özcan¹,

Demir²,

Yılmaz³

et al. 2019

BioDiCon

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Glyphosate is a widely used broad-spectrum herbicide. Vanilic acid is a phenolic acid found naturally in many plants. In this study, the effect of vanilic acid against oxidative stress induced by glyphosate in Saccharomyces cerevisiae was investigated. S. cerevisiae was proliferated and developed in YEDP medium. Both glyphosate and vanilic acid were added to the development environment of Saccharomyces cerevisiae to be 200, 400 and 800 mg per liter. At the end of the experiment, Saccharomyces cerevisiae samples (Reduced glutathione (GSH), oxidized glutathione (GSSG), Malondialhedit (MDA), fatty acid, E, D, K vitamins and phytosterol) were analyzed in HPLC and GC devices. Antioxidant potential of vanillic acid was also identified. In this study, the effect of vanillic acid against oxidative stress and toxicity caused by glyphosate in S. cerevisiae was investigated for the first time. In S. cerevisiae administered glyphosate, there were statistically significant changes in MDA, GSH, GSSG, protein, fatty acid, E, D, K vitamins and phytosterol levels, but the vanilic acid applied was detected statistically significantly reduced the changes in these parameters. As a result of analysis by gas chromatography, the yeast cell of octanoic acid (8: 0), lauric acid (12: 0), myristic acid (14: 0), palmitic acid (16: 0), palmitoleic acid (16:1 n-7), stearic acid (18: 0), oleic acid (18:1 n-9) and linoleic acid (18:2 n-6) acids were observed. Vitamin D, E and K were analyzed with HPLC device. As a result of this analysis, molecules such as K2, δ-tocopherol, D2, D3, α-tocopherol, ergosterol, K1, stigmasterol, β-sitosterol were identified. Biochemical analysis showed that cell density in culture medium containing 800 mg per liter of glyphosate decreased. It was found that vanilic acid showed beneficial effects against oxidative stress caused by glyphosate in Saccharomyces cerevisiae.

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Maternal glyphosate-based herbicide exposure alters antioxidant-related genes in the brain and serum metabolites of male rat offspring

Cited by 33 publications

References 76 publications

In Silico Exploration of the Potential Role of Acetaminophen and Pesticides in the Etiology of Autism Spectrum Disorder

In Silico Exploration of the Potential Role of Acetaminophen and Pesticides in the Etiology of Autism Spectrum Disorder

Intergenerational thyroid hormone homeostasis imbalance in cerebellum of rats perinatally exposed to glyphosate‐based herbicide

Effect of vanillic acid against oxidative stress induced by glyphosate in Saccharomyces cerevisiae

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