Maternal-Fetal Outcome, Lipid Profile and Oxidative Stress of Diabetic Rats Neonatally Exposed to Streptozotocin

Damasceno, Débora Cristina; Kiss, Ana Carolina Inhasz; Sinzato, Yuri Karen; Campos, Kleber Eduardo de; Rudge, Marilza; Calderon, Iracema de Mattos Paranhos; Volpato, Gustavo Tadeu

doi:10.1055/s-0030-1269886

Cited by 25 publications

(30 citation statements)

References 27 publications

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“…Nevertheless, a number of studies carried out in our laboratory found increased rates of pre-and postimplantation embryo losses and embryonic death (resorption), reduced number of live fetuses, and maternal weight gain during diabetic pregnancy. These alterations are due to metabolic changes caused by hyperglycemic peaks during pregnancy (Kiss et al 2009;Damasceno et al 2011;Iessi et al 2010;Saito et al 2010;Dallaqua et al 2012;Sinzato et al 2012). The divergent results shown in our study, which does not reinforce an effect of diabetes on the reproductive performance of rats, is possibly explained by mating time.…”

Section: Discussioncontrasting

confidence: 83%

“…The rates of preimplantation embryo loss were calculated by the formula: (number of corpora lutea− number of implantations)×100/number of corpora lutea. The rates of postimplantation loss were calculated by the formula: (number of implantations−number of live fetuses×100)/number of implantations (Damasceno et al 2008). When embryo or fetal development could not be observed, the uterus was stained by the Salewski's technique (Salewski 1964) for marking possible implantation points.…”

Section: Pregnancymentioning

confidence: 99%

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Extracellular HSP70 levels in diabetic environment in rats

Santos

Sinzato

Gallego

et al. 2015

Cell Stress and Chaperones

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The expression of HSP70 in embryonic cells of mammals and its role for their normal development and protection is an important aspect to be investigated in pregnancy and/or mild diabetes. In this sense, the present study evaluated the effects of mild diabetes on maternal reproductive parameters and HSP70 levels in Wistar rats at different stages of life and in their offspring. Mild diabetes was induced by a betacytotoxic drug (streptozotocin) at birth. Four experimental groups were evaluated: at 90 days of age: nonpregnant nondiabetic (ND90) and nonpregnant mild diabetic (D90) female rats, and at term pregnancy: pregnant female rats of both glycemic status were examined (NDP and DP, respectively). The rats were submitted to oral glucose tolerance test, and blood samples were collected for determination of HSP70 levels. In addition, the reproductive performance of pregnant rats was assessed and HSP70 levels determined in their offspring blood samples. The HSP70 levels and maternal reproductive performance presented no difference between ND and D rats, regardless of the life stage. The HSP70 levels were increased in D90 rats and lower in offspring from D rats. Maternal HSP70 levels were positively correlated to the number of dead embryos. In conclusion, mild diabetes did not affect maternal reproductive performance, but high maternal HSP70 levels compromised embryo development. In addition, offspring from D rats exhibited lower HSP70 levels, showing that this protein can be used as an indicator of metabolic consequences of diabetes and predictor of related disorders in adulthood.

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Section: Discussioncontrasting

confidence: 83%

Section: Pregnancymentioning

confidence: 99%

Extracellular HSP70 levels in diabetic environment in rats

Santos

Sinzato

Gallego

et al. 2015

Cell Stress and Chaperones

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“…The umbilical cord was clamped by using Halsteadtype hemostatic clamps. The rate of embryonic loss before and after implantation was calculated 24 . If there was a lack of visible implantation sites, the uterine corns were stained with a preparation of 10% ammonium sulfate 25 .…”

Section: Methodsmentioning

confidence: 99%

Effect of exercise on the maternal outcome in pregnancy of spontaneously hypertensive rats

Rocha

Peraçoli

Volpato³

et al. 2014

Acta Cir. Bras.

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PURPOSE:To evaluate the effect of exercise (swimming) on pregnancy in spontaneously hypertensive rats (SHR). METHODS:Thirty three pregnant female SHR were distributed into three groups (n=11 animals/group): SHR Control=non-exercised (sedentary); SHR Ex0 = exercised from day zero to day 20 of pregnancy; and SHR Ex7 = exercised from day 7 to 20 of pregnancy. Body weight and systolic blood pressure were indirectly measured during pregnancy. On gestational day 21, the rats were anaesthetized and uterine content was withdrawn for analysis of maternal reproductive outcome parameters and fetal development. RESULTS:The reduced blood pressure percentage was higher in SHR Ex0 and SHR Ex7 compared to SHR Control group. Weight gain was present in all pregnancy periods, but it was lower in SHR Ex7 than in SHR Control dams. The exercise increased the preimplantation loss rate. The post-implantation loss rate was lower in SHR Ex0 group. SHR Ex7 group showed a significantly higher percentage of fetuses classified as small for gestational age as compared to others groups. CONCLUSION:The exercise contributed to lowering gestational blood pressure in SHR rats, but had a negative impact on the developing embryo.

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“…Moreover, hydroureter may or may not be associated with congenital hydronephrosis, it might possibly be a transitory variation in rodents (Taylor 1986). According to the literature, approximately 20% of newborns of normal rats have this kind of visceral anomaly (Damasceno et al 2002b(Damasceno et al , 2011a.…”

Section: Citrus Aurantium Effects In Pregnant Ratsmentioning

confidence: 99%

Effect of essential oil from Citrus aurantium in maternal reproductive outcome and fetal anomaly frequency in rats

Volpato

Francia-Farje

Damasceno

et al. 2015

An. Acad. Bras. Ciênc.

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Citrus aurantium L., commonly known as bitter orange, is widely used in folk medicine, but there is little data in the literature about the effects on pregnancy. The aim of the present study was to evaluate the influence of essential oil obtained from fruits of Citrus aurantium on the maternal reproductive outcome and fetal anomaly incidence in rats. Pregnant Wistar rats were randomized into four groups (n minimum = 12 animals/group): G1 = control, G2 to G4 = treated with essential oil from C. aurantium at dose 125, 250 and 500 mg/kg, respectively. Rats were orally treated, by gavage, with plant essential oil or vehicle during pre-implantation and organogenic period (gestational day 0-14). On gestational day 20 the rats were anaesthetized and the gravid uterus was weighed with its contents and the fetuses were analyzed. Results showed that the treated group with 500 mg/kg presented decreased placental weights and placental index, although the treatment with bitter orange essential oil did not show any alteration in maternal reproductive performance, toxicological effect, changes in ossification sites, and malformation index. In conclusion, the treatment of Citrus aurantium essential oil was not teratogenic and did not alter the maternal reproductive outcome.

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Maternal-Fetal Outcome, Lipid Profile and Oxidative Stress of Diabetic Rats Neonatally Exposed to Streptozotocin

Cited by 25 publications

References 27 publications

Extracellular HSP70 levels in diabetic environment in rats

Extracellular HSP70 levels in diabetic environment in rats

Effect of exercise on the maternal outcome in pregnancy of spontaneously hypertensive rats

Effect of essential oil from Citrus aurantium in maternal reproductive outcome and fetal anomaly frequency in rats

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