Maternal allergy modulates cord blood hematopoietic progenitor Toll-like receptor expression and function

Impaired lipopolysaccharide responsiveness of cord blood mononuclear cells and the risk of asthma: a longitudinal study

“…In addition, their definition of atopy was based on an MD diagnosis. Other studies support the role of LPS in modulating atopic diseases and asthma by demonstrating that polymorphisms in CD14 and TLR-4, which form part of the LPS-TLR signaling complex, correlated with the presence of atopy (1,(24)(25)(26)(27). In one longitudinal study of a highrisk cohort, an enhanced CBMC cytokine response to LPS was associated with nonatopic wheezing at the age of 2 y (15).…”

IL-4 and IL-13 Differentially Regulate TLR-Induced Eosinophil-Basophil Differentiation Of Cord Blood CD34+ Progenitor Cells

“…+ cells express reduced TLRs with muted LPS-induced Eo/B CFU [10], compared to low-risk infants. Since LPS can induce Eo/B CFU from CD34…”

“…Our group has extensively investigated the role of hematopoietic progenitor cells in infant CB in relation to allergic risk and development of disease [7][8][9][10]. We have recently shown that the presence of maternal atopy alters CB progenitor toll-like receptor (TLR) phenotype and function; at-risk infant CD34…”

“…+ cells via autocrine activation of MAPK [11] and atopic at-risk infants have elevated T H 2 cytokines in their CB [12,13], we were interested in what effect these cytokines may have on LPS-induced Eo/B CFU [10]. Relatedly, maternal cytokines (which can be transferred to the CB) have been shown to play instructive roles in fetal immune development.…”

The Pros and Cons of Airway Lining Fluid Composition Analysis

“…In addition to minimizing discomfort, it avoids allergen challenge and nasal lavage, the confounding effect of the variable dilution of analytes during lavage, and the possibility that dilution may result in mediator concentrations lower than the detection limit of the assay (9, 10). Another strength is that the sample being studied is from a target organ of allergic disease rather than assaying cytokines and chemokines in stimulated cord or peripheral blood, as has been done previously in newborns (11)(12)(13). Further, use of principal component analysis is a strength.…”