Materials, workflows and applications of IMAC for phosphoproteome profiling in the recent decade: A review

Tang, Ruizhi; Bai, Quan; Ma, Shujuan; Ou, Junjie

doi:10.1016/j.trac.2022.116862

Cited by 9 publications

(7 citation statements)

References 198 publications

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“…Researches have revealed a strong association between cancer and abnormal phosphorylation, therefore, phosphoproteomics can provide significant insight into the early detection and treatment of cancer. 8 Endogenous phosphopeptides in human serum are important biomarkers of cancer; however, their direct detection faced serious interference due to the presence of soluble proteins. Therefore, the enrichment materials for human serum should be able to directly enrich phosphopeptide under the interference of proteins.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…Liquid biopsy, especially serum, is increasingly recognized as a promising noninvasive identification method of clinical biomarkers. Researches have revealed a strong association between cancer and abnormal phosphorylation, therefore, phosphoproteomics can provide significant insight into the early detection and treatment of cancer . Endogenous phosphopeptides in human serum are important biomarkers of cancer; however, their direct detection faced serious interference due to the presence of soluble proteins.…”

Section: Resultsmentioning

confidence: 99%

“…In the “shotgun” strategy, proteins are digested by enzymes for analysis in mass spectrometry. The low abundance and dynamics of phosphopeptides make the enrichment of phosphopeptides an integral part of the phosphoproteomics analysis workflow . In recent years, based on typical enrichment strategies, such as immobilized metal ion affinity chromatography (IMAC), − metal oxide affinity chromatography (MOAC), − and immunoprecipitation, − some emerging functional enrichment materials were discovered, including dual-functional materials that could simultaneously enrich different types of post-translational modification (PTM) peptides, − dual-mode materials that integrating different enrichment strategies, , and reusable materials that pursue the concept of green chemistry.…”

Section: Introductionmentioning

confidence: 99%

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Biobased Tannic Acid-Chitosan Composite Membranes as Reusable Adsorbents for Effective Enrichment of Phosphopeptides

Tang,

Pan,

Bai

et al. 2023

Langmuir

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High-performance reusable materials from renewable resources are rare and urgently required in bioseparation. Herein, a series of tannic acid-chitosan composite membranes for the enrichment of phosphopeptides were fabricated by the freeze casting method. First, a tannic acid-chitosan composite membrane was acquired via the multiple hydrogen bonds between tannic acid and chitosan, which had a long-range aligned three-dimensional microstructure. Second, a covalent–hydrogen bond hybrid composite was also fabricated, with stable and aligned honeycomb-like microstructures that formed by the synergy of covalence and hydrogen bonding. Besides, a ternary composite membrane was “one-pot” synthesized by the copolymerization of tannic acid, chitosan, and Ti4+ ions, indicating the feasibility of involving metal ions in the composition of the polymer skeleton in place of additional modification steps. The as-prepared chitosan composite membranes exhibited excellent performance in the enrichment of phosphopeptides from β-casein tryptic digest and human serum. Benefitting from the long-range aligned honeycomb-like structure coordinated by hydrogen bonds and covalent bonds, and a large number of pyrogallol functional groups provided by tannic acid, the covalent–hydrogen bond hybrid membrane showed excellent reusability and could be reused up to 16 times in phosphopeptide enrichment, as far as we know, which is the best reported result to date.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biobased Tannic Acid-Chitosan Composite Membranes as Reusable Adsorbents for Effective Enrichment of Phosphopeptides

Tang,

Pan,

Bai

et al. 2023

Langmuir

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“…The method for phosphate-based IMAC or metaloxide (typically using TiO 2 ) enrichment has been well established as a commonly used strategy in proteomics analysis. 46,47 We first reported a phosphate-based enrichable MS-cleavable and multi-targeting protein cross-linker, pBVS (11, Fig. 6), in which an oxygen-phosphorus bond was inserted, just like the bond in phosphopeptides.…”

Section: Chemical Reagents For the Biotin-avidin Systemmentioning

confidence: 99%

Chemical reagents for the enrichment of modified peptides in MS-based identification

Huangfu,

Yu,

Sun

et al. 2024

Chem. Commun.

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“…It is estimated that more than two-thirds of human proteins are phosphorylated, resulting in thousands of potential sites in cells to regulate protein function. − Studies of smart responsive materials for phosphopeptide enrichment have been focused on temperature or pH response. In our previous work, we developed a dual light- and pH-responsive SP-based magnetic adsorbent, demonstrating the feasibility of SP materials for phosphopeptide enrichment.…”

Section: Introductionmentioning

confidence: 99%

Light, pH, and Temperature Triple-Responsive Magnetic Composites for Highly Efficient Phosphopeptide Enrichment

Zhao

Zheng

et al. 2023

Anal. Chem.

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Smart materials can dynamically and reversibly change their structures and functions in response to external stimuli. In this study, we designed a smart magnetic composite (MNP-pSPA-b-pNIPAm) with a triple response to ultraviolet (UV) light, pH, and temperature. Relying on the response of spiropyranyl acrylate (SPA) and N-isopropylacrylamide (NIPAm) to external stimuli (light, pH, and temperature), MNP-pSPA-b-pNIPAm was used for the controlled capture and release of phosphopeptides. The established phosphopeptide enrichment platform exhibits high sensitivity (detection limit of 0.04 fmol), high selectivity (BSA/β-casein, 1000:1), and good reusability (6 cycles). In addition, the method was also applied to the enrichment of phosphopeptides in real samples (skim milk, human saliva, and serum), demonstrating the feasibility of this method for phosphoproteomic analysis. After enriching from human nonsmall cell lung cancer cell (A549) lysates with MNP-pSPA-b-pNIPAm, 2595 phosphopeptides corresponding to 2281 phosphoproteins were identified. The novel responsive enrichment probe is highly specific for phosphoproteomic analysis and provides an effective method for studying the significance of protein phosphorylation in complex biological samples.

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Materials, workflows and applications of IMAC for phosphoproteome profiling in the recent decade: A review

Cited by 9 publications

References 198 publications

Biobased Tannic Acid-Chitosan Composite Membranes as Reusable Adsorbents for Effective Enrichment of Phosphopeptides

Biobased Tannic Acid-Chitosan Composite Membranes as Reusable Adsorbents for Effective Enrichment of Phosphopeptides

Chemical reagents for the enrichment of modified peptides in MS-based identification

Light, pH, and Temperature Triple-Responsive Magnetic Composites for Highly Efficient Phosphopeptide Enrichment

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