Materials used in regenerative endodontic procedures and their impact on tooth discoloration

Fagogeni, Irini; Metlerska, Joanna; Lipski, Mariusz; Falgowski, Tomasz; Górski, M.; Nowicka, Alicja

doi:10.2334/josnusd.18-0467

Cited by 23 publications

(27 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Hydraulic cements based on calcium silicate composition are now well‐established clinical options when approaching pulp or periapical reparation/regeneration due to their excellent antimicrobial properties, sealing capacity, biocompatibility and bioactivity (Fagogeni et al . 2019, Giacomino et al . 2019).…”

Section: Introductionmentioning

confidence: 99%

Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow

et al. 2020

View full text Add to dashboard Cite

AimTo evaluate in a laboratory setting the effects of Endosequence BC Sealer HiFlow (Brasseler USA, Savannah, GA, USA), a novel calcium silicate‐based sealer developed for use in warm canal filling techniques, on human periodontal ligament stem cells (hPDLSCs).MethodologyEluates of EndoSequence BC Sealer HiFlow (BCHiF) (Brasseler USA), EndoSequence BC Sealer (BCS) (Brasseler USA) and AH Plus (AHP) (Dentsply DeTrey GmbH, Konstanz, Germany) were placed in contact with hPDLSCs. The characterization of the chemical elements of the root canal sealers was assessed using scanning electron microscopy and energy‐dispersive X‐ray analysis (SEM‐EDX). Inductively coupled plasma‐mass spectrometry (ICP‐MS) was used to determine the ion release of the sealers. MTT assay and wound healing techniques were used to determine cell viability and migration, respectively. Cell morphology and cell attachment were assessed using a direct contact technique of hPDLSCs onto the surface of the sealers and analysed by SEM. The bioactivity potential was carried out with the Alizarin Red and qPCR testing methods. The statistical differences were evaluated using one‐way anova and Tukey’s test (P < 0.05).ResultsICP‐MS and EDX revealed significantly more zirconium in BCHiF than BCS (P < 0.05), whereas BCS had slightly higher levels of Ca2+ than BCHiF (P < 0.05). The cell viability assay revealed no relevant differences between BCS and BCHiF when compared with the control group (P > 0.05). Both BCS and BCHiF had similar rates of cell migration to the control group at 24 and 48 h. Cell morphology and adhesion capacity were also similar for BCS and BCHiF groups, whilst the AHP group was associated with reduced adhesion capacity. The Alizarin Red assay revealed a significant difference between the BCS and the control group (P < 0.001), as well as for the BCHiF group (P < 0.001). Finally, BCS and BCHiF promoted overexpression of osteo/cementogenic genes.ConclusionsIn general, EndoSequence BC Sealer HiFlow possesses suitable biological properties to be safely used as a root canal filling material and promote increased expression of oste/cementogenic genes by hPDLSCs.

show abstract

Section: Introductionmentioning

confidence: 99%

Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Regenerative medicine applies multidisciplinary biology and engineering science to therapeutic approaches to regenerate, replace, or repair tissues and organs [1]. In the field of dentistry, regenerative medicine has been used for the treatment of complex diseases in which oral cavity and dental tissues are damaged or lost, including endodontic treatments [2], periodontics [3], and other disciplines. One of the most promising areas of regenerative medicine is tissue engineering, which combines human cells with biocompatible biomaterials or scaffolds in order to generate tissue substitutes able to promote tissue regeneration [4].…”

Section: Introductionmentioning

confidence: 99%

In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

et al. 2020

View full text Add to dashboard Cite

Recent advances in tissue engineering offer innovative clinical alternatives in dentistry and regenerative medicine. Tissue engineering combines human cells with compatible biomaterials to induce tissue regeneration. Shortening the fabrication time of biomaterials used in tissue engineering will contribute to treatment improvement, and biomaterial functionalization can be exploited to enhance scaffold properties. In this work, we have tested an alternative biofabrication method by directly including human oral mucosa tissue explants within the biomaterial for the generation of human bioengineered mouth and dental tissues for use in tissue engineering. To achieve this, acellular fibrin–agarose scaffolds (AFAS), non-functionalized fibrin-agarose oral mucosa stroma substitutes (n-FAOM), and novel functionalized fibrin-agarose oral mucosa stroma substitutes (F-FAOM) were developed and analyzed after 1, 2, and 3 weeks of in vitro development to determine extracellular matrix components as compared to native oral mucosa controls by using histochemistry and immunohistochemistry. Results demonstrate that functionalization speeds up the biofabrication method and contributes to improve the biomimetic characteristics of the scaffold in terms of extracellular matrix components and reduce the time required for in vitro tissue development.

show abstract

“…The mean values of L*, a*, and b* were taken and the color change (∆E*) of each specimen was calculated for each interval using the following equation [ 20 ]: L* parameter indicates lightness from 0 (black) to 100 (white); a* represent red (+ 80a*) to green (− 80a*); and b* represent yellow (+ 80b*) to blue (− 80b*) [ 16 ]. Color change (ΔE) values beyond 3.7 were considered clinically perceptible [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…The manufacturers of these products are claiming that their products have the ability to overcome the drawbacks of MTA including tooth discoloration potential [ 11 – 13 ]. Thus, the discoloration effect of the various calcium silicate-based materials was of interest to the dental clinicians and researchers, and many of the review studies have addressed this topic concluding that; some of the these materials have a high potential of tooth discoloration, while some have much less discoloration effect, and this variation was related to the differences in the compositions of these materials, therefore, they recommended more laboratory and clinical research studies in this field [ 14 – 16 ].…”

Section: Introductionmentioning

confidence: 99%

The effect of different calcium silicate-based pulp capping materials on tooth discoloration: an in vitro study

2021

View full text Add to dashboard Cite

Background Variation in the composition of calcium silicate-based pulp capping materials could influence the discoloration potential of some of these materials, thus affecting the color and aesthetic appearance of the coronal tooth structure. Furthermore, contact with blood if hemostasis is not fully achieved may enhance this discoloration for some materials. Therefore the aim of this study was to evaluate in vitro the color change of coronal tooth structure after placing various calcium silicate-based materials in the pulp chamber in the presence or absence of blood. Mehtods Maxillary extracted premolars (n = 144) were sectioned and the crowns were separated from the roots. Pulp chambers were prepared to a standard size and then the tested materials (GMTA Angelus, ProRoot WMTA, Biodentine, TheraCal, and TotalFill) were placed with saline or with blood. Color change was assessed by spectrophotometry; prior to and after material placement at different time intervals of 24 h, 1 week, 1 month, 3 months, and 6 months. Color change (ΔE) values were calculated and statistically analyzed. Results In the saline groups, Biodentine caused the least color change, while GMTA and WMTA caused the highest color change which were significantly different from the others (p < 0.001), TotalFill and TheraCal caused moderate changes. Adding blood increased the ΔE overall the tested materials to various degrees. Biodentine was the most affected by the blood, while MTA groups were the least affected, followed by TotalFill and then TheraCal. The increase in ΔE was significant over time up to 3 months, after which the increase was not significant. Conclusions Overall, WMTA and GMTA caused the most severe discoloration. In saline, Biodentine caused the least discoloration, but it was the most affected by the presence of blood, although it still caused the least discoloration similar to that observed with TotatFill. TheraCal caused moderate discoloration but more than that caused by Biodentine and TotalFill.

show abstract

Materials used in regenerative endodontic procedures and their impact on tooth discoloration

Cited by 23 publications

References 27 publications

Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow

Chemical composition and bioactivity potential of the new Endosequence BC Sealer formulation HiFlow

In Vitro Generation of Novel Functionalized Biomaterials for Use in Oral and Dental Regenerative Medicine Applications

The effect of different calcium silicate-based pulp capping materials on tooth discoloration: an in vitro study

Contact Info

Product

Resources

About