Mastitis pathogens and somatic cell count in ewes milk

Abstract

The aim of this study was to determine the occurrence of pathogens in selected group of ewes and the relationship between somatic cell count (SCC) and the presence of pathogens. The experiment was carried out on a dairy farm, where predominantly breed was a Tsigai. Sampling was carried out in monthly intervals as part of the milk recording test day from February to July 2019. A total of 303 ewes were included in the survey, during the milk recording test day. The ewes with SCC ≥1000 × 103 cells.mL-1 were selected for further sampling at half udder level. Based on SCC the ewes were divided into five groups: <200 ×103; ≥200 <400 × 103; ≥400 <600 × 103; ≥600 <1000 × 103; ≥1000 × 103 cells.mL-1. The first group of SCC contained 33.9% of milk samples, the second 14.1% of samples, the third 5.7% of samples, the fourth 6.2% and the fifth 40.1% of samples. The most common pathogens were coagulase negative staphylococci (CNS). The most frequent CNS was Staphylococcus (S.) simulans (24.4%). S. aureus was identified in 5.3% of bacteriological positive samples. Almost 70% of ewes with bacteriological positive samples were repeated identified the presence of pathogens during tested period. SCC ≥500 × 103 cells.mL-1 were detected in 92.5% bacteriological positive milk samples. The presence of pathogens increased SCC in milk (p <0.001) as compared to samples free of pathogens. In conclusion, the SCC ≥500 × 103 cells.mL-1 could be important for detection of subclinical mastitis at half udder level in dairy ewes.

Identification of Staphylococcus Spp. Isolated From Food by Two Methods

“…However, it also notes that while MALDI-TOF-MS has revolutionized speed and precision of microbial identification for clinical isolates, in contrast few performance studies have been published so far focusing on suitability for particularly industrial applications. In addition to CoNS (Tvarožková et al, 2020), the species S. aureus (Zigo et al 2011;Tančin et al 2017;Holko et al 2018) is often identified in milk and dairy products, similarly to our study where in bryndza its presence was confirmed by both methods.…”

Maintaining Optimal Mammary Gland Health and Prevention of Mastitis

“…Many of them can be classified as functional foods in different geographical and social localities. However, European Union regulations oblige producers to obtain milk only form healthy animals in order to increase consumers safety, what however may limit milk production and consumption ( 1 – 4 ).…”

The presence of pathogens in milk of ewes in relation to the somatic cell count and subpopulations of leukocytes

“…The overall prevalence of the bacteriological positive milk samples was 36.73% from separate udder halves of ewes. Vasileiou et al (2018) detected 26% occurrence of subclinical mastitis and other study found 36.1% prevalence of subclinical mastitis (Tvarozkova et al 2020). The negative bacteriological samples formed 60.20% of the total tested samples.…”

“…The milk samples at half udder level were divided based on somatic cell count to five SCC groups: SCC1 < 200 000 cells/ml (45 milk samples); 200 000 ≤ SCC2 < 400 000 cells/ml (10 milk samples); 400 000 ≤ SCC3 < 600 000 cells/ml (six milk samples); 600 000 ≤ SCC4 < 1 000 000 cells/ml (six milk samples); SCC5 ≥ 1 000 000 cells/ml (31 milk Czech Journal of Animal Science, 66, 2021 ( 08 (Tvarozkova et al 2020). In the study of Ergun et al (2009), the occurrence of S. aureus was observed in 3.1% of milk samples without clinical signs.…”