Masterminding B Cells

Cory, Suzanne

doi:10.4049/jimmunol.1501277

Cited by 2 publications

(1 citation statement)

References 37 publications

(32 reference statements)

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“…B cells are an important part of the adaptive immune system, arising from hematopoietic stem cell precursors. These cells express surface immunoglobulin (Ig) receptors and secrete these same proteins as antibodies into the serum after differentiation into plasma cells [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing

et al. 2018

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Antibody specificity and diversity are generated through the enzymatic splicing of genomic gene segments within each B cell. Antibodies are heterodimers of heavy- and light-chains encoded on separate loci. We studied the antibody repertoire from pooled, splenic tissue of unimmunized, adult female C57BL/6J mice, using high-throughput sequencing (HTS) without amplification of antibody transcripts. We recovered over 90,000 heavy-chain and over 135,000 light-chain immunoglobulin sequences. Individual V-, D-, and J-gene segment usage was uniform among the three mouse pools, particularly in highly abundant gene segments, with low frequency V-gene segments not being detected in all pools. Despite the similar usage of individual gene segments, the repertoire of individual B-cell CDR3 amino acid sequences in each mouse pool was highly varied, affirming the combinatorial diversity in the B-cell pool that has been previously demonstrated. There also was some skewing in the V-gene segments that were detected depending on chromosomal location. This study presents a unique, non-primer biased glimpse of the conventionally housed, unimmunized antibody repertoire of the C57BL6/J mouse.

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Section: Introductionmentioning

confidence: 99%

Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing

et al. 2018

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Phenotypic and genotypic infidelity in B-lineage neoplasms, including transdifferentiation following targeted therapy: Report from the 2021 SH/EAHP Workshop

Goodlad

Xiao

Amador

et al. 2023

American Journal of Clinical Pathology

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Objectives Session 2 of the 2021 Society for Hematopathology and European Association for Haematopathology Workshop collected examples of lineage infidelity and transdifferentiation in B-lineage neoplasms, including after targeted therapy. Methods Twenty cases were submitted. Whole-exome sequencing and genome-wide RNA expression analysis were available on a limited subsample. Results A diagnosis of B-cell acute lymphoblastic leukemia (B-ALL) was rendered on at least 1 biopsy from 13 patients. There was 1 case of acute myeloid leukemia (AML); the remaining 6 cases were mature B-cell neoplasms. Targeted therapy was administered in 7 cases of B-ALL and 4 cases of mature B-cell neoplasms. Six cases of B-ALL underwent lineage switch to AML or mixed-phenotype acute leukemia at relapse, 5 of which had rearranged KMT2A. Changes in maturational state without lineage switch were observed in 2 cases. Examples of de novo aberrant T-cell antigen expression (n = 2) were seen among the mature B-cell lymphoma cohort, and their presence correlated with alterations in tumor cell gene expression patterns. Conclusions This cohort of cases enabled us to illustrate, discuss, and review current concepts of lineage switch and aberrant antigen expression in a variety of B-cell neoplasms and draw attention to the role targeted therapies may have in predisposing neoplasms to transdifferentiation as well as other, less expected changes in maturational status.

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Masterminding B Cells

Abstract: MaterialSupplementary http://www.jimmunol.org/content/suppl/2015/07/17/195.3.763.DC1

Cited by 2 publications

References 37 publications

Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing

Characterization of the naive murine antibody repertoire using unamplified high-throughput sequencing

Phenotypic and genotypic infidelity in B-lineage neoplasms, including transdifferentiation following targeted therapy: Report from the 2021 SH/EAHP Workshop

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