Mast cells in the swimbladder of Atlantic salmon Salmo salar:histochemistry and responses to compound 48/80 and formalin-inactivated Aeromonas salmonicida

Reite, Ola B.; Evensen, Øystein

doi:10.3354/dao020095

Cited by 30 publications

(30 citation statements)

References 13 publications

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“…The latter authors described degranulation of eosinophilic granular cells in swim bladders and peritoneum following injection with bacteria. These cells have been known to occur in salmonid tissue, where they respond to various bacterial products (Ellis 1985, Vallejo & Ellis 1989, Powell et al 1993, Reite & Evensen 1994. In addition, the present work shows that thionin-postive cells occur in brown trout fin epidermis, where they seem to degranulate upon infection with skin parasites.…”

Section: Discussionsupporting

confidence: 47%

“…The exact nature of these cells is not clear but due to the metachromatic staining with thionin following fixation in absolute ethanol they fit the description of teleost mast cells provided by Reite (1998). Morphologically they corresponded to the swim bladder mast cells described by Reite & Evensen (1994) and to similarly stained cells seen by control staining of rainbow trout swim bladder performed by the present authors (data not shown). The function of these putative mast cells in the trout fin are unknown.…”

Section: Discussionmentioning

confidence: 57%

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Associations between epidermal thionin-positive cells and skin parasitic infections in brown trout Salmo trutta

Sigh¹,

Buchmann²

2000

Dis. Aquat. Org.

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The dynamics of the densities of epidermal thionin-positive cells (putative mast cells) in the skin of brown trout fry were investigated during experimental infections with the skin parasites Ichthyophthirius multifilus (Ciliophora) and Gyrodactylus derjavini (Monogenea). It was shown that the metachromatic thionin-stained cells were extremely sensitive to parasite exposure, as the density of cells in the skin of trout decreased markedly after exposure to the pathogens. As early as 7 d post infection the cell counts were significantly reduced and almost totally depleted following 9 d infection, which suggests that degranulation of the cells occurs following parasite exposure. No recruitment of new cells was seen during the study period. Some reduction in uninfected control groups indicates that the putative mast cells are sensitive to stress as well. A notable variation in densities of thionin-stained cells between different fins was found and the cornea1 surface was devoid of these cells. The possible implications of these cells in host-parasite interactions are suggested and discussed.

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Section: Discussionsupporting

confidence: 47%

Section: Discussionmentioning

confidence: 57%

Associations between epidermal thionin-positive cells and skin parasitic infections in brown trout Salmo trutta

Sigh¹,

Buchmann²

2000

Dis. Aquat. Org.

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“…Later, the inflammatory response was evaluated by ink injection (Ellis et al, 1976), complete Freund's adjuvant and Staphylococcus aureus (Finn and Nielsen, 1971), carrageenin (Timur et al, 1977), Escherichia coli lipopolysaccharide (LPS), complete Freund's adjuvant, turpentine and carrageenin (White et al, 1981), Vibrio alginolyticus (MacArthur et al, 1984), liquid paraffin (Suzuki, 1986), Edwardsiella ictaluri LPS (Saeed and Plumb, 1986), Pseudomonas alcaligenes and Aeromonas punctata (Siwicki and Studnicka, 1987), Aeromonas salmonicida (Reite and Evensen, 1994), LPS (Brunetti et al, 1994), Freund's adjuvant and squalene (Jenkins and Klesius, 1998), microcystin (Vajcová et al, 1998), Escherichia coli , peptidoglican (Kono and Sakai, 2001), beta-glucan of Saccharomyces cerevisiae and Salmonella typhimurium LPS (Paulsen et al, 2001) and Mycobacterium marinum (Gauthier et al, 2003). In these studies, the inflammation was characterized by showing macrophages, leucocytes, fibroblasts and fibroplasia.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

Martins

Myiazaki²,

Tavares‐Dias

et al. 2009

Braz. J. Biol.

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This work evaluated the acute inflammatory response induced by injections of 0.5 mL saline solution (control), 500 µg carrageenin and 0.5 mL thioglycollate 3% in the swim bladder of juvenile tambacu hybrid. Fish were distributed in three treatments, three replications and acclimated for a period of 10 days before assay. The cell characterization from the inflammatory exudate was performed in Giemsa and PAS stained smears. Carrageenin, injected in fish, showed an increase on the total number of cells in the inflammatory exudate when compared to saline and thioglycollate injected. Whereas, for carrageenin-injected fish, the percentage of thrombocyte was higher than thioglycollate. On the other hand, granulocyte percentage in thioglycollate-injected fish was higher than the ones injected using carrageenin. Carrageenin provoked the highest migration of macrophage to the inflammatory site. The PAS method confirmed the presence of three types of granulocytes: eosinophilic granular cell (EGC) type 1 with the characteristics of a special granulocytic cell commonly found in the circulating blood; EGC type 2 shorter than the last one and neutrophil. This study contributes to a better understanding of the inflammatory response and infectious processes in native fish.Keywords: fish, hybrid tambacu, inflammation, exudate, carrageenin, thioglycollate. Caracterização da resposta inflamatória aguda no híbrido tambacu (Piaractus mesopotamicus macho × Colossoma macropomum fêmea) (Osteichthyes) ResumoEste estudo avaliou a resposta inflamatória aguda induzida por injeções de 0,5 mL de solução salina (controle), 500 µg de carragenina e 0,5 mL de tioglicolato a 3% na bexiga natatória de juvenis do híbrido tambacu. Os peixes foram distribuídos em três tratamentos, três repetições e aclimatados durante 10 dias antes do ensaio. A caracterização das células do exsudato inflamatório foi feita após coloração com Giemsa e PAS. Peixes injetados com carragenina apresentaram maior número de células no exsudato inflamatório do que com salina e tioglicolato. A porcentagem de trombócitos no exsudato foi maior nos injetados com carragenina quando comparada com a dos injetados com tioglicolato. Por outro lado, o percentual de granulócitos foi maior em animais injetados com tioglicolato do que em animais injetados com carragenina. A carragenina provocou maior migração de macrófagos para o foco inflamatório. O método de PAS Injection and collection of exudate cellsThe fish were anaesthetized by immersion in benzocaine solution (1 g.10 L -1 ) and injected with 500 µg carrageenin (Marine Colloids) dissolved in 0.5 mL sterile saline solution, 0.5 mL thioglycollate 3% and 0.5 mL sterile saline solution (control). After injection, the fish were maintained in the aquaria for 6 hours until the sacrifice. The interior of the swim bladder was washed with complete phosphate buffered saline containing 0.01 mL EDTA 5% and cell suspensions were centrifuged at 150 × gravity for 10 minutes before staining. The total number of cells was determined with a ha...

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“…The results obtained in the present study suggest that neutrophil migration stimulating factor(s) are released by degranulation of EGCs in tilapia. (Ellis, 1985;Vallejo and Ellis, 1989;Powell et al, 1991Powell et al, , 1993Reite and Evensen, 1994;Reite 1997Reite , 1998, and the accumulation of leucocytes after degranulation of the EGCs have been reported (Reite, 1997;Reite and Evensen, 1994;Matsuyama and lida, 1999 a).…”

Section: Cells and Its Effect On Neutrophil Migrationmentioning

confidence: 99%

In Vitro Degranulation of Tilapia Eosinophilic Granular Cells and its Effect on Neutrophil Migration.

Matsuyama

Iida

2000

Fish Pathol.

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ABSTRACT-Migration of neutrophils toward the supernatant of degranulated eosinophilic granu lar cells (EGCs) was examined using a modified Boyden chamber.EGCs of tilapia (Oreochromis niloticus) were isolated from the swim bladder membrane, and degranulated by incubation with sub stance P (neurotransmitter) or compound 48/80. The degranulation of the EGCs was also observed after incubation with normal tilapia sera containing zymosan, but not with heat-inactivated sera. Additionally, significantly higher neutrophil migration was observed when the degranulation of the EGCs occurred in the lower compartment of a Boyden chamber.High-speed centrifugation inhibited the degranulation of the EGCs stimulated by substance P or zymosan plus normal tilapia sera, and migration of neutrophils was inhibited. The results obtained in the present study suggest that neutrophil migration stimulating factor(s) are released by degranulation of EGCs in tilapia.

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Mast cells in the swimbladder of Atlantic salmon Salmo salar:histochemistry and responses to compound 48/80 and formalin-inactivated Aeromonas salmonicida

Cited by 30 publications

References 13 publications

Associations between epidermal thionin-positive cells and skin parasitic infections in brown trout Salmo trutta

Associations between epidermal thionin-positive cells and skin parasitic infections in brown trout Salmo trutta

Characterization of the acute inflammatory response in the hybrid tambacu (Piaractus mesopotamicus male × Colossoma macropomum female) (Osteichthyes)

In Vitro Degranulation of Tilapia Eosinophilic Granular Cells and its Effect on Neutrophil Migration.

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