Mast cells in atopic dermatitis

Kawakami, Toshiaki; Ando, Tomoaki; Kimura, Miho; Wilson, Bridget S.; Kawakami, Yuko

doi:10.1016/j.coi.2009.09.006

Cited by 301 publications

(284 citation statements)

References 148 publications

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“…Many skin disorders like AD seem to be related to infiltration and activation of mast cells, resulting in skin inflammation [30] [34]. In the current study, total mast cell counts increased significantly after 21 days of sensitization (Figure 4).…”

Section: Discussionmentioning

confidence: 59%

“…AD appears to be associated with inflammatory immune dysregulation of the skin, impaired skin barrier function, and IgE-mediated sensitization to food and environmental allergens such as microbial pathogens [29] [30]. Such immunologic disturbance is reflected in elevated serum IgE levels due to increased inflammatory cell infiltration into the skin, which is type2 helper T-cell-dominated, and, according to the immune dysregulation hypothesis, the defective skin barrier is interpreted as a consequence of inflammation [2].…”

Section: Discussionmentioning

confidence: 99%

“…Such immunologic disturbance is reflected in elevated serum IgE levels due to increased inflammatory cell infiltration into the skin, which is type2 helper T-cell-dominated, and, according to the immune dysregulation hypothesis, the defective skin barrier is interpreted as a consequence of inflammation [2]. Regardless of how to explain disease progression, the acute eczematous lesions exhibit spongiosis, hyperkeratosis and parakeratosis, and the chronic lesions are characterized by acanthosis and perivascular infiltration of lymphocytes and mast cells, and by lichenification of the skin, infiltration of Th1 cells, and tissue remodeling with increased collagen deposition and dermal thickening [30]. From this point of view, the elevation of serum IgE levels in this NC/ Nga mouse AD model was related to the onset of AD-like skin lesion development [31] [32], and also similar to that reported in AD patients with IgE hyperproduction [33].…”

Section: Discussionmentioning

confidence: 99%

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Topical Application of Cudrania tricuspidata Stem Extract Inhibits Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model: An Experimental Animal Study

Park¹,

Kim²,

Kim³

et al. 2016

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Atopic dermatitis (AD) is a chronic inflammatory skin disease characterized by elevated immunoglobulin E (IgE), mast cell infiltration and skin lesions including pruritus, erythema and eczema. Cudrania tricuspidata extracts have been clinically administered for a long time in the East Asia including Korean and China as a home-remedy to diminish the inflammation of gastritis and hepatitis. To examine whether it works on AD or not, an AD-like animal model was experimented in this study. AD was induced by applying Dermatophagoides farinae (D. farinae) extract to the backs of 9-week old NC/Nga mice for 21 days. Following this, an ethanol extract of C. tricuspidata stems (EECT) was applied topically for 14 days to the sensitized skin, while distilled water was used as a control (EECT0 mice). Anti-AD effects of EECT were evaluated using scores for AD-like skin lesions, serum IgE levels and mast cell counts in the skin dermal layers to assess inflammation. Topically applied ethanol extract of Cudrania tricuspidata stems (EECT 7.5, 25 and 75 mg/mL) markedly reduced AD-like skin lesions after 4 days (by 30.1%, 31.4% and 38.5%, respectively) and also after * Corresponding author.Y.-S. Park et al. 35914 days (by 63.6%, 66.1% and 49.6%, respectively), while distilled water improved AD by 17.8% and 38.7%, respectively (p < 0.05). Serum IgE production was reduced in the EECT7.5, EECT25 and EECT75 groups after 4 days (by 57.6%, 65.9% and 59.3%, respectively) and after 14 days of the treatment (by 82.0%, 79.6% and 75.3%, respectively), while distilled water decreased it by 38.8% and 62.3% (p = 0.0001 and p = 0.0001, respectively). Mast cell counts increased after sensitization by D. farinae extract (p = 0.003) and EECT attenuated the mast cell overproduction, and reduced mast cell degranulation markedly. Attenuation was most obvious in the early stage of EECT treatment when the AD was most acute.

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Section: Discussionmentioning

confidence: 59%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Topical Application of Cudrania tricuspidata Stem Extract Inhibits Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model: An Experimental Animal Study

Park¹,

Kim²,

Kim³

et al. 2016

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“…29) In our study, hypertrophy of the epidermis was confirmed with PAS staining, and hyperkeratosis was also checked with immunohistochemistry, using anti-cytokeratin 10 antibody. Epidermal thickness and cytokeratin 10 differentiation were followed as a similar pattern.…”

Section: Discussionmentioning

confidence: 99%

Functional polysaccharides from Grifola frondosa aqueous extract inhibit atopic dermatitis-like skin lesions in NC/Nga mice

Park

Hwang

Kim

et al. 2015

Bioscience, Biotechnology, and Biochemistry

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Grifola frondosa (GF), distributed widely in far east Asia including Korea, is popularly used as traditional medicines and health supplementary foods, especially for enhancing the immune functions of the body. To extend the application of GF polysaccharides (GFP) for atopic dermatitis (AD), we investigated the effects of GFP on the 2,4-dinitrochlorobenzene-induced AD-like skin lesion in NC/Nga mice. GFP treatment significantly reduced the dorsa skin dermatitis score and combination treatment with GFP, and dexamethasone has a synergistic effect in AD-like skin lesion by reduced Serum IgE, mast cells infiltration, and cytokines expression. These results indicate that GFP suppressed the AD-like skin lesions by controlling the Th-1/Th-2-type cytokines in NC/Nga mice. These findings strongly suggest that GFP can be useful for AD patients as a novel therapeutic agent and might be used for corticosteroids replacement or supplement agent.

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“…Similarly, mast cells are equipped with the high affinity receptor FcεRI for IgE, the antigen of which is known to induce mast cell degranulation and initiate hypersensitivity, both of which are seen in atopic dermatitis (Kawakami et al, 2009). Histamine, a preformed inflammatory mediator, induces scratching and thus aggravates the symptoms of atopic dermatitis.…”

Section: Seamentioning

confidence: 99%

The Effect of Extract from Sea Buckthorn on DNCB-induced Atopic Dermatitis in NC/Nga Mice

Park¹,

Shin²,

Yang³

et al. 2012

Korean Journal of Plant Resources

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-Sea Buckthorn (Hippophae rhamnoides L.) has been used in traditional medicine for the treatment of cough, indigestion, circulatory problems and pain. The associated anti-inflammatory effect of this agent is achieved via the inhibition of Nf-ĸB signaling, a property that has been demonstrated to effectively control the symptoms of various skin disorders, including atopic dermatitis. Accordingly, the purpose of this study was to assess the efficacy of Sea Buckthorn in reducing the production of lipopolysaccharide (LPS) activated nitric oxide (NO) by inhibiting the Nf-κB pathway, as measured by the symptoms of atopic dermatitis (AD) occurring secondarily to inflammation and immune dysregulation. Our data demonstrate that Sea Buckthorn significantly decreased the LPS-induced production of NO (p<0.001). Atopic dermatitis was induced by repeated application of 2,4-dinitrochlorobenzene to the dorsal skin of mice. Topical application of 5% Sea Buckthorn extract improved the symptoms of AD, specifically reducing disease severity scores, scratching behaviors and epidermal thickness. When compared to the control group, animals treated with Sea Buckthorn exhibited increased serum IL-12 levels and decreased serum TNF-α, IL-4 and IL-5 levels. Such a modulation of biphasic T-helper (Th)1/Th2 cytokines may result in a reduction in serum IgE levels. Our findings suggest that mechanism of action of Sea Buckthorn in the treatment of AD is associated with a marked anti-inflammatory effect as well as an inhibition of Th2-mediated IgE overproduction via the modulation of biphasic Th1/Th2 cytokines. Such results suggest that topical Sea Buckthorn extract may prove to be a novel therapy for AD symptoms with few side effects.

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Mast cells in atopic dermatitis

Cited by 301 publications

References 148 publications

Topical Application of Cudrania tricuspidata Stem Extract Inhibits Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model: An Experimental Animal Study

Topical Application of Cudrania tricuspidata Stem Extract Inhibits Atopic Dermatitis-Like Skin Lesions in an NC/Nga Mouse Model: An Experimental Animal Study

Functional polysaccharides from Grifola frondosa aqueous extract inhibit atopic dermatitis-like skin lesions in NC/Nga mice

The Effect of Extract from Sea Buckthorn on DNCB-induced Atopic Dermatitis in NC/Nga Mice

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