Mast Cells Are Required in the Proliferation and Remodeling Phases of Microdeformational Wound Therapy

Abstract

Mast cells are critical in the robust granulation tissue response seen in microdeformational wound therapy and in the modulation of the remodeling phase of wound healing.

The Role of Mouse Mast Cell Proteases in the Proliferative Phase of Wound Healing in Microdeformational Wound Therapy

“…They form intimate connections with keratinocytes and fibroblasts through gap junctions. 7 It has been shown that mast cells are involved in all three phases of wound healing 8,9 -the inflammatory, proliferative, and remodeling phases 10 -however, the exact mechanism of mast cell involvement has not been fully revealed. 11 The release of the granule contents (exocytosis) is dependent on the types of receptors on the mature mast cell and on the activating agents in the respective tissue.…”

“…12 Mast cells can degranulate in response to various biochemical and immunogenic agents (e.g., immunoglobulin E, complement proteins, and growth factors 13,14 ) and in response to physical stimuli, which include sunlight (sunlight is really electromagnetic radiation), radiation, trauma, and mechanical stretch. 7 Of note, mast cells possess transient receptor potential vanilloid channels on their membranes that are activated by mechanical stimulation and lead to calcium influx through specific ion channels, which eventually causes the mast cell to degranulate. 15 Microdeformational wound therapy, also referred to as negative-pressure wound therapy, uses a porous polyurethane foam under vacuum to induce microdeformations on the wound edge 16,17 and is used clinically for the treatment of complex wounds.…”

“…15 Microdeformational wound therapy, also referred to as negative-pressure wound therapy, uses a porous polyurethane foam under vacuum to induce microdeformations on the wound edge 16,17 and is used clinically for the treatment of complex wounds. 7 These devices accelerate healing and granulation tissue formation, 18,19 and mast cells are integral to the effect of microdeformational wound therapy on wound healing. 7 Because microdeformation involves stretch, it may be that the activation of these mast cells occurs by means of the transient receptor potential vanilloid proteins, which would result in the release of mast cell mediators.…”

“…7 These devices accelerate healing and granulation tissue formation, 18,19 and mast cells are integral to the effect of microdeformational wound therapy on wound healing. 7 Because microdeformation involves stretch, it may be that the activation of these mast cells occurs by means of the transient receptor potential vanilloid proteins, which would result in the release of mast cell mediators.…”

Flavonoids in propolis acting on mast cell-mediated wound healing

“…Wound healing is a complex process of lysis and reconstitution controlled by a series of cell signaling proteins and involved tissue regeneration and angiogenesis (Hiromatsu and Toda 2003;Nienartowicz et al 2006). Mast cells have been shown to play a significant role in the early inflammatory stage of wound healing and also influence proliferation and tissue remodeling in skin; according to some authors in wound healing, mast cells are able to modulate only the inflammatory but not the proliferative response in healing and reconstituting damaged tissue (Egozi et al 2003), while other reports showed that mast cells are involved in tissue remodeling in the late phase of wound healing (Iba et al 2004), thus expanding the debate about the role of mast cells in tissue remodeling (Kennelly et al 2011;Younan et al 2011;Garner and Ehrlich 2011). The role of inflammatory cells, including those factors commonly associated with allergy such as basophils and mast cells, is involving many other unexpected fields, such as cancer (Heneberg 2011) and obesity (Zhang and Shi 2012).…”