Mast Cell Biology: Introduction and Overview

Gilfillan, Alasdair M.; Austin, Sarah J.; Metcalfe, Dean D.

doi:10.1007/978-1-4419-9533-9_1

Cited by 122 publications

(108 citation statements)

References 73 publications

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“…Hence, Kit W-sh/W-sh mice, in which surface expression of Kit or Kit catalytic activity is defective, have significantly reduced mast cell numbers. 23 In the present study, we used mast cell-deficient Kit W-sh/W-sh mice to determine the involvement of mast cells in DENV infection and disease.…”

Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

Mast cell–macrophage dynamics in modulation of dengue virus infection in skin

et al. 2015

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SummaryDengue virus (DENV) infection causes dengue fever, dengue haemorrhagic fever, or dengue shock syndrome. Mast cells have been speculated to play a role in DENV disease although their precise roles are unclear. In this study, we used mast cell-deficient Kit W-sh/W-sh mice to investigate the involvement of mast cells after intradermal DENV infection. An approximately two-to three-fold higher level of DENV NS3 antigen was detected at the skin inoculation site in DENV-infected Kit W-sh/W-sh mice than in DENV-infected wild-type (WT) mice (using a dose of 1 3 10 9 plaqueforming units/mouse). Moreover, as an indicator of heightened pathogenesis, a more prolonged bleeding time was observed in DENV-infected Kit W-sh/W-sh mice than in WT mice. Monocytes/macrophages are considered to be important targets for DENV infection, so we investigated the susceptibility and chemokine response of DENV-infected peritoneal macrophages from Kit W-sh/W-sh and WT mice both ex vivo and in vivo. There was a tendency for higher DENV infection and higher secretion of CCL2 (MCP-1) from peritoneal macrophages isolated from Kit W-sh/W-sh mice than those from WT mice. In vivo studies using intradermal inoculation of DENV showed about twofold higher levels of infiltrating macrophages and CCL2 (MCP-1) at the inoculation site in both mock control and DENV-inoculated Kit W-sh/W-sh mice than in corresponding WT mice. In summary, compared with WT mice, Kit W-sh/W-sh mice show enhanced DENV infection and macrophage infiltration at the skin inoculation site as well as increased DENV-associated bleeding time. The results indicate an intriguing interplay between mast cells and tissue macrophages to restrict DENV replication in the skin.

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Section: M M U N O L O G Y O R I G I N a L A R T I C L Ementioning

confidence: 99%

Mast cell–macrophage dynamics in modulation of dengue virus infection in skin

et al. 2015

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“…histamine, proteases, proteoglycans, metalloproteinases), de novo generated and secreted arachidonic acid metabolites [i.e. leukotrienes (LTs), prostaglandins (PGs) and thromboxanes (TXs)] as well as cytokines and chemokines [1,2]. These mediators elicit pro-inflammatory, anti-inflammatory and immunoregulatory effects and act directly on various cell populations.…”

Section: Introductionmentioning

confidence: 99%

“…Mast cells are particularly abundant beneath subepithelial layers of the skin, in the airways, gastrointestinal and genitourinary tracts, thus close to the external environment, virtually at the portals of infection [1,2]. Furthermore, these cells express pathogen recognition receptors (PRRs), including Toll-like receptors (TLRs) [11], that detect bacteria-associated specific molecules [12].…”

Section: Introductionmentioning

confidence: 99%

Toll-like receptors 3 ligation directly and indirectly affects mast cell cysteinyl leukotriene generation

Witczak¹,

Pietrzak²,

Słodka³

et al. 2013

cejoi

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“…Mast cells also stained for KIT, the SCF receptor that is required for mast cell development. 8 Microdissection ( Figure 1C-F) was performed on frozen sections that were immunostained for the mast cell marker tryptase ( Figure 1C-D) or the stromal cell marker inhibin-A ( Figure 1E-F). The distinct morphology and staining patterns of the mast and stromal cells allowed for clear identification of the cell populations under study.…”

mentioning

confidence: 99%

“…Erythrocytes, granulocytes, and mast cells all derive from a common myeloid progenitor. KIT, a key receptor in mast cell development 8 and erythropoiesis, 9 has been observed in hemangioblastomas. 5 The Kit signaling pathway may contribute to the ability of some of the VHL-deficient pluripotent neoplastic cells to develop into more differentiated progeny, including mast cells and erythrocytes.…”

mentioning

confidence: 99%