Toll-like receptors 3 ligation directly and indirectly affects mast cell cysteinyl leukotriene generation

Witczak, Piotr; Pietrzak, Anna; Słodka, Aleksandra; Brzezińska‐Błaszczyk, Ewa

doi:10.5114/ceji.2013.37755

Cited by 1 publication

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References 31 publications

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“…Only TLR2, TLR3, and TLR4 proteins were found in mast cells from isolated human nasal polyps [22], murine peritoneal cavity [38], and FSMC [30]. Our previous studies have shown that freshly isolated rat peritoneal mast cells express both surface TLR2 and TLR4 molecules [20, 39], as well as TLR3 and TLR7 surface proteins [40, 41]. In this study we evaluated the expression of TLR receptors in native fully mature rat mast cells freshly isolated from the peritoneal cavity, that is connective tissue type mast cells.…”

Section: Discussionmentioning

confidence: 99%

Expression of surface and intracellular Toll-like receptors by mature mast cells

Agier¹,

Żelechowska²,

Kozłowska³

et al. 2016

cejoi

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Nowadays, more and more data indicate that mast cells play an important role in host defense against pathogens. That is why it is essential to understand the expression of Toll-like receptors (TLRs) by mast cells, because these molecules play particularly significant role in initiation host defense against microorganisms as they recognize both wide range of microbial pathogen-associated molecular patterns (PAMPs) and various endogenous damage-associated molecular patterns (DAMPs) released in response to infection. Therefore, we examined the constitutive expression of both surface and endosomal TLRs in rat native fully mature tissue mast cells. By the use of qRT-PCR we found that these cells express mRNAs for TLR2, TLR3, TLR4, TLR5, TLR7, and TLR9. The expression of TLR3, TLR4, TLR5, TLR7, and TLR9 transcripts were low and comparable and only the expression of TLR2 transcript was significant. By the use of flow cytometry technique, we clearly documented that mast cells express TLR2, TLR4, and TLR5 on cell surface, while TLR3, TLR7, and TLR9 proteins are located both on the cell membrane and intracellularly. The highest expression was observed for TLR5 and the lowest for surface TLR7. These observations undoubtedly indicate that mature tissue mast cells have a broad set of TLR molecules, thus can recognize and bind bacterial, viral, and fungal PAMPs as well as various endogenous molecules generated in response to infection.

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