Massilia flava sp. nov., isolated from soil

Abstract

A Gram-stain-negative, rod-shaped bacterium, designated strain Y9 T , was isolated from a soil sample collected in Ningxia Province in China and was characterized to determine its taxonomic position. Strain Y9 T contained Q-8 as the predominant ubiquinone. Major fatty acid components were summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH) and C 16 : 0 . The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The G+C content of the genomic DNA of strain Y9 T was 68.7 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that the strain fell within the evolutionary radiation encompassed by the genus Massilia. Levels of 16S rRNA gene sequence similarity between strain Y9 T and the type strains of recognized Massilia species ranged from 95.2 to 98.2 %, the highest values being with Massilia albidiflava 45 T (98.2 %) and Massilia lutea 101 T (98.0 %). However, levels of DNA-DNA relatedness between strain Y9 T and M. albidiflava KCTC 12343 T and M. lutea KCTC 12345 T were 37 and 26 %, respectively. Strain Y9 T was clearly differentiated from its nearest phylogenetic relatives in the genus Massilia based on phenotypic, chemotaxonomic and phylogenetic properties. Therefore, strain Y9 T is considered to represent a novel species of the genus Massilia, for which the name Massilia flava sp. nov. is proposed. The type strain is Y9 T

Drivers of the composition of active rhizosphere bacterial communities in temperate grasslands

“…This is consistent with the capacity for coumaric acid utilization by several members of this genus, such as A. hydrophila, A. sobria, and A. caviae [75]. Furthermore, Sphingobium paulinellae, algicola, and scionense [76,77] utilize Nacetylglucosamine and Massilia flava has tryptophane deaminase activity [78]. The congruence with known physiological traits of described genera indicates that our combined approach can also provide information on the substrate adaptations of not-yet-cultured rhizosphere bacteria.…”

Illumina MiSeq sequencing investigation on the contrasting soil bacterial community structures in different iron mining areas

“…The relative values for bacterial genera are indicated by color intensity with the legend indicated at the top right corner been reported to utilize testosterone under both oxic and anoxic conditions. The genus Massilia was mainly isolated from the blood of an immunocompromised patient (Lindquist et al 2003) and has been recently found in environmental samples, such as soil samples from China and in the drinking water distribution system in Seville (Spain) (Gallego et al 2006;Wang et al 2012a). In this study area, Steroidobacter was found to closely relate to Cd content (R 2 =0.729, P<0.05), while Massilia had significant correlation with Cd and Cr contents (R 2 =0.718, P<0.05; R 2 =0.671, P<0.05).…”

Massilia norwichensis sp. nov., isolated from an air sample

“…The genus Massilia La Scola et al 2000 belongs to the betaproteobacterial family Oxalobacteraceae , together with the closely related genera Telluria , Pseudoduganella , Duganella and Janthinobacterium (Kämpfer et al , 2011; Wang et al , 2012; Kong et al , 2013). The genus was described on the basis of a single clinical isolate from the blood of a 25-year-old immunocompromised patient with meningoencephalitis, which was assigned to the type species Massilia timonae (La Scola et al , 1998).…”