Mass transfer mechanisms in Hyper D media for chromatographic protein separation

Rendueles, Manuel; Chenou, C.; Loureiro, José M.; Rodrigues, Alírio E.

doi:10.1016/s1369-703x(97)00003-x

Cited by 49 publications

(2 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A mobile phase (5 g/L yeast dissolved in 20 mM phosphate buffer, pH 7.4 or 20 mM sodium phosphate buffer, pH 7.4) was used to measure column pressure drop at different interstitial velocities. Column permeability (

κ

) was calculated from the relationship between pressure drop and velocity (Quinn, 2014; Rendueles de la Vega et al, 1998).

κ = \frac{u \times η \times L}{∆ P} = \frac{ε \times u_{f} \times η \times L}{∆ P}

where

∆ P

is the pressure drop across the column and

η

is the mobile‐phase viscosity.…”

Section: Methodsmentioning

confidence: 99%

Performance of various 3D‐printed monolith geometries as an alternative to expanded bed adsorption for protein purification

Pei

Feast²,

Holland

et al. 2023

Biotech & Bioengineering

View full text Add to dashboard Cite

Here, we compare the performance of various three‐dimensional‐printed Monolith Adsorption (PMA) columns designed from a triply periodic minimal surface geometry, the Schoen gyroid. The structures examined had designed hydraulic diameters between 203 and 458 µm and voidages of 40%−60%. We compare column efficiency, porosity, static binding capacity and dynamic binding capacity for various load volumes and flow rates. The results show that all structures allowed efficient passage of yeast cells (>97%) over a wide range of interstitial velocities (191 to 1911 cm/h) while maintaining a low pressure drop (<0.1 MPa). The structure with a voidage of 40% and a hydraulic diameter of 203 µm showed the best performance in all aspects evaluated. Bovine serum albumin (BSA) recoveries for all structures (27%−91% when the loaded volume was 180 mL) were significantly affected by hydraulic diameter, mean channel wall thickness, velocity and voidage. Moreover, biomass addition resulted in a decrease in BSA recovery, which became more obvious at high velocities. However, this did not lead to a dramatic reduction in saturated binding capacity, significant changes in axial dispersion, or blockage of channels and could be compensated for by recirculation of the feed, even at high velocity. PMA thus potentially provides an appealing alternative to Expanded Bed Adsorption, retaining the latter's advantages, while eliminating fluidization issues and minimizing both processing time and buffer consumption.

show abstract

κ

) was calculated from the relationship between pressure drop and velocity (Quinn, 2014; Rendueles de la Vega et al, 1998).

κ = \frac{u \times η \times L}{∆ P} = \frac{ε \times u_{f} \times η \times L}{∆ P}

where

∆ P

is the pressure drop across the column and

η

is the mobile‐phase viscosity.…”

Section: Methodsmentioning

confidence: 99%

Performance of various 3D‐printed monolith geometries as an alternative to expanded bed adsorption for protein purification

Pei

Feast²,

Holland

et al. 2023

Biotech & Bioengineering

View full text Add to dashboard Cite

show abstract

“…The permeability and mechanical strength of the monoliths were described by hydrodynamics of the column using 20% ethanol solution as a mobile phase at 25 °C on a Waters preparative liquid chromatography system (Waters 1525 HPLC, USA). For a column with a length of L , the bed permeability ( K ) of the monolith can be calculated by Darcy’s law in a laminar flow region where μ is the viscosity of the mobile phase (Pa·s), u is the superficial velocity (m/s), and Δ P is the column pressure drop (Pa).…”

Section: Methodsmentioning

confidence: 99%

Synthesis of Bimodal Open-Porous Polystyrene Monoliths with Glycopolymer Surfaces for High-Speed Protein Chromatography

Lin

Liu

et al. 2021

ACS Appl. Polym. Mater.

View full text Add to dashboard Cite

Conventional polystyrene (PS) monoliths are rarely used for separation of biomacromolecules due to their heterogeneous skeletons and hydrophobic surfaces. In this work, highpermeable and bimodal open-porous polystyrene monoliths (BOPMs) with homogeneous skeletons and glycopolymer surfaces were prepared by reversible addition−fragmentation chain transfer (RAFT) polymerization via an emulsion-templating method. The stable and bicontinuous medium-internal phase emulsion (MIPE) was stabilized by the cooperative assembly of two surfactants, that is, a self-made amphiphilic diblock glycopolymer (ADG) in the oil phase and Pluronic F127 (PF127) in the water phase. It was found that the higher hydrophilic−lipophilic balance (HLB) value of ADGs needs to match the higher concentration of PF127 to promise the homogeneous structure of the bicontinuous MIPE. A possible mechanism was proposed based on the hydrogen bonding interaction. Using the MIPE as a template, BOPMs were successfully synthesized by RAFT emulsion polymerization. The effects of the HLB value and adding amount of ADGs on the morphology of the BOPM were also studied. There are two sets of pores in the BOPM determined by mercury porosimetry, that is, gigapores (200−7000 nm) and mesopores (20−100 nm). Taking advantage of glycopolymer surfaces of the BOPM, glycoprotein can be directly separated at a mobile-phase velocity of up to 1806 cm/h in the hydrophilic interaction liquid chromatography mode. The BOPM as prepared shows good permeability, mechanical stability, and biocompatibility, which is an ideal support in high-speed protein chromatography, cell culture, and enzyme immobilization.

show abstract

High‐Performance Liquid Chromatography of Biological Macromolecules

Irgum¹

2000

Encyclopedia of Analytical Chemistry

View full text Add to dashboard Cite

Mass transfer mechanisms in Hyper D media for chromatographic protein separation

Cited by 49 publications

References 37 publications

Performance of various 3D‐printed monolith geometries as an alternative to expanded bed adsorption for protein purification

Performance of various 3D‐printed monolith geometries as an alternative to expanded bed adsorption for protein purification

Synthesis of Bimodal Open-Porous Polystyrene Monoliths with Glycopolymer Surfaces for High-Speed Protein Chromatography

High‐Performance Liquid Chromatography of Biological Macromolecules

Contact Info

Product

Resources

About