In this study, we report on a novel composite membrane system for pH‐responsive controlled release, which is composed of a porous membrane with linear grafted, positively pH‐responsive polymeric gates acting as functional valves, and a crosslinked, negatively pH‐responsive hydrogel inside the reservoir working as a functional pumping element. The proposed system features a large responsive release rate that goes effectively beyond the limit of concentration‐driven diffusion due to the pumping effects of the negatively pH‐responsive hydrogel inside the reservoir. The pH‐responsive gating membranes were prepared by grafting poly(methacrylic acid) (PMAA) linear chains onto porous polyvinylidene fluoride (PVDF) membrane substrates using a plasma‐graft pore‐filling polymerization, and the crosslinked poly(N,N‐dimethylaminoethyl methacrylate) (PDM) hydrogels were synthesized by free radical polymerization. The volume phase‐transition characteristics of PMAA and PDM were opposite. The proposed system opens new doors for pH‐responsive “smart” or “intelligent” controlled‐release systems, which are highly attractive for drug‐delivery systems, chemical carriers, sensors, and so on.
Bioassay-guided separation of the antifungal constituents of the Chinese liverwort Marchantia polymorpha L. (Marchantiaceae) led to the isolation of seven bis[bibenzyl]-type macrocycles. On the basis of NMR and MS analyses, the three new compounds plagiochin E (1), 13,13'-O-isoproylidenericcardin D (4), and riccardin H (7) were identified, together with four known compounds: marchantin E (2), neomarchantin A (3), marchantin A (5), and marchantin B (6). Their antifungal activities against Candida albicans were determined by TLC bioautography.
Natural variations in pH levels of tissues in the body make it an attractive stimuli to trigger drug release from a delivery vehicle. A number of such carriers have been developed but achieving high drug loading combined with low leakage at physiological pH and tunable controlled release at the site of action is an ongoing challenge. Here we report a novel strategy for the synthesis of entirely hydrophilic stimuli-responsive nanocarriers with high passive loading efficiency of doxorubicin (DOX), which show good stability at pH 7 and rapid tunable drug release at intracellular pH. The particles (D = 120-150 nm), are prepared by cross-linking the core of swollen micelles of the triblock copolymer poly[poly(ethylene glycol) methyl ether methacrylate-b-N,N'-di(methylamino)ethyl methacrylate-b-tert-butyl methacrylate] (poly(PEGMEM A)-b- PDMAEMA-b-PtBMA)). After subsequent deprotection of the tert-butyl groups a hydrophilic poly(methacrylic acid) (PMAA) core is revealed. Due to the negative charge in the acidic core the particles absorb 100% of the DOX from solution at pH 7 at up to 50 wt % DOX/polymer, making them extremely simple to load. Unlike other systems, the DMAEMA "gating" shell ensures low drug leakage at pH 7, whereas physical shrinkage of the MAA core allows rapid release below pH 6. The particles deliver DOX with high efficiency to human pancreatic cancer AsPC-1 cell lines, even lowering the IC50 of DOX. As the particles are stable as a dry powder and can be loaded with any mixture of positively charged drugs without complex synthetic or purification steps, we propose they will find use in a range of delivery applications.
To overcome the disadvantages of protein denaturation and nonspecific adsorption on poly(styrene-divinylbenzene) (PS) medium as a chromatographic support, gigaporous PS microspheres prepared in our previous study were coated with hydrophobically modified agarose (phenoxyl agarose, Agap). Both the modification of agarose and the gigaporous structure of PS microspheres provided an advantage that facilitated the coating of Agap onto PS microspheres. The amount of Agap adsorbed onto the PS surface was examined as a function of the polymer concentration, and various samples of microspheres, differing in surface Agap density, were prepared. The adsorbed layer was then stabilized by chemical cross-linking and its stability was evaluated in the presence of sodium dodecyl sulfate. Results showed that PS microspheres were successfully coated with Agap, while the gigaporous structure could be well maintained. After coating, the nonspecific adsorption of proteins on PS microspheres was greatly reduced. Flow hydrodynamics experiments showed that the Agap-co-PS column had low backpressure, good permeability, and mechanical stability. Such a procedure could provide a hydrophilic low-pressure liquid chromatographic support for different types of chromatography, since the Agap layer may be easily derivatized by classical methods, and because of their good permeability, the coated microspheres have great potential applications in high-speed protein chromatography.
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