Mass spectrometry of the cannabinoids and their metabolites

Harvey, David J.

doi:10.1002/mas.1280060104

Cited by 55 publications

(41 citation statements)

References 197 publications

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“…22 However, the use of a matrix introduces a number of problems into the MALDI-MS analysis and one major limitation is the unsatisfying variability of signal intensities between different desorption spots across the preparation. 23 Therefore, an oen time-consuming search for "sweet spots" is necessary in order to yield good signal-to-noise ratios or an analyte signal at all.…”

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confidence: 99%

A facile method for cellular N-glycomic profiling by matrix-assisted laser desorption/ionization mass spectrometry

et al. 2017

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Conventional protocols for cellular N-glycan profiling often need several time-consuming and laborintensive steps, and a large amount of material (5-20 Â 10 6 cells) is required. In this study, an optimized co-derivatization method was applied for convenient, rapid and highly-sensitive analysis of cellular Nglycan. Taking human cervical carcinoma cells (HeLa) as a model, the required amount for comprehensive cellular N-glycans analysis was reduced down to an original cell number of 10 5 and the total analysis time was decreased from several days to several hours. Good reproducibility of the method was also obtained with the CV averaging to less than 13.1%. In addition, compared to permethylation derivatization, more than 5-fold sensitivity improvement was achieved and more concise and clear mass spectra were obtained with the new developed method. As a preliminary study, this method was also

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confidence: 99%

A facile method for cellular N-glycomic profiling by matrix-assisted laser desorption/ionization mass spectrometry

et al. 2017

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“…Metabolites containing biotransformation at C-7 were identified by the presence of a major ion resulting from loss of this group (16).…”

Section: Metabolite Identificationmentioning

confidence: 99%

Metabolism of cannabidiol by the rat

Samara

Bialer

Harvey

1991

European Journal of Drug Metabolism and Pharmacokinetics

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Metabolites of CBD excreted into the bile and perfusion fluid were examined in a rat liver perfusion preparation. Metabolites were extracted with ethyl acetate and identified by GC/MS as TMS derivatives. Four mono- and five di-hydroxy metabolites were identified with major sites of metabolic attack being at C-7 and C-4". A hydroxy-ketone was detected but not fully identified. All biliary metabolites were conjugated with glucuronic acid. Urinary metabolites were studied in rats with samples taken at times to 25 h after drug administration. Unmetabolized CBD and 13 metabolites were identified by GC/MS. Major metabolites were acids with beta-oxidation being a prominent pathway. The 6- and 7-hydroxy derivatives of 4",5"-bis,nor-CBD-3"-oic acid were the most abundant compounds but substantial concentrations of the di-acids, CBD-5",7-dioic acid and 4",5"-bis,nor-CBD-3",7-dioic acid were present. Concentrations of the more highly oxidized metabolites increased with time.

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“…The column oven was temperature-programmed from 180·C to 300·C at 4·C1min. Other operating conditions for electron-impact mass spectrometry Metabolites were identified by GClMS and by comparison with literature data (10)(11)(12)(13)(14). Most of the metabolites of this cannabinoid were mono-hydroxy compounds as shown by the molecular ions at mlz 470 and the very abundant [M -CH3]+ ions in the mass spectra of their TMS derivatives.…”

Section: Gas Chromatographymentioning

confidence: 99%

“…These both shifted by 1.8 mass units in the spectra of the [2ag]-TMS derivatives (9). The positions of the hydroxy groups in the pentyl side-ehain were determined by the presence of the diagnostic ions described earlier (10,13). The peaks attributed to these metabolites were absent from the profiles from control animals not treated with the drug.…”

Section: Gas Chromatographymentioning

confidence: 99%

In vitro metabolism of cannabinol in rat, mouse, rabbit, guinea pig, hamster, gerbil and cat

Brown

Harvey

1990

Eur. J. Drug Metab. Pharmacokinet.

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Metabolism of cannabinol (CBN) was studied in hepatic microsomal incubates from mouse, rat, rabbit, guinea pig, cat, hamster and gerbil. Metabolites were extracted with ethyl acetate, concentrated by chromatography on Sephadex LH-20 and identified by GC/MS as TMS derivatives. Six monohydroxy metabolites were identified. These had hydroxy groups at C-11 and at all positions of the pentyl side-chain. Metabolism varied considerably between the species. 11-Hydroxylation was the most prominent route in the majority of species, but in the hamster and cat the major metabolic pathway was 4'-hydroxylation. Metabolites hydroxylated in the pentyl chain were generally more abundant in guinea pig, hamster and cat.

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Mass spectrometry of the cannabinoids and their metabolites

Cited by 55 publications

References 197 publications

A facile method for cellular N-glycomic profiling by matrix-assisted laser desorption/ionization mass spectrometry

A facile method for cellular N-glycomic profiling by matrix-assisted laser desorption/ionization mass spectrometry

Metabolism of cannabidiol by the rat

In vitro metabolism of cannabinol in rat, mouse, rabbit, guinea pig, hamster, gerbil and cat

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