Mass spectrometry of complex lipids

Kuksis, A.

doi:10.1007/978-1-4613-1131-7_6

Cited by 19 publications

(3 citation statements)

References 181 publications

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“…All of the results were obtained with a relative standard deviation (RSD %) lower than 6%, the majority of RSD % values being around 2−3%. The results for the fatty acid content in the analyzed samples of common oils is in excellent agreement with data reported in the literature (see, e.g., refs − ). For the seeds of Nicotiana species, all the oils except for those from N. suaveolens had a composition somewhat similar to that of safflower oil and grape seed oil.…”

Section: Resultssupporting

confidence: 91%

Dual Analysis of Triglycerides from Certain Common Lipids and Seed Extracts

Moldoveanu

Chang

2011

J. Agric. Food Chem.

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A number of reference oils, two commercial oils, and several oil extracts from seeds of Nicotiana species were analyzed for the fatty acid content and also for triglyceride composition. The seed oils were obtained using an accelerated solvent extraction procedure, which was proven to be very efficient and reproducible. The fatty acids were analyzed after the hydrolysis of the oils, using trimethylsilylation and gas chromatography/mass spectrometry (GC/MS) analysis. The levels of sixteen molecular species of triglycerides in the oils were measured after GC separation using MS for identification and flame ionization detection (FID) for quantitation. The results for the fatty acids and those for triglycerides were combined to generate uniform information regarding the composition of the analyzed oils. For a number of oils, the individual triglyceride quantitation and mass spectra were reported for the first time. The study showed that in some cases, oils with similar fatty acid content do not have the same triglycerides profile. The fatty acids and triglycerides profile for selected Nicotiana species were described for the first time in the literature.

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Section: Resultssupporting

confidence: 91%

Dual Analysis of Triglycerides from Certain Common Lipids and Seed Extracts

Moldoveanu

Chang

2011

J. Agric. Food Chem.

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“…The TLC-FID ester peak was constituted by the coelution of steryl and aliphatic esters. First, the molecular distribution of steryl esters, for the two fractions obtained, was determined by using subambient pressure GC-CI-MS since steryl esters, and particularly cholesteryl esters are thermally labile (24). Steryl esters present in wool wax can be determined by their sterol composition (i.e., lanosterol, dihydrolanosterol, or cholesterol), acid moiety, and isomeric form, namely, normal, iso and anteiso, with iso corresponding to (ω-1)-monomethyl-substituted FFA and anteiso to (ω-2)-monomethyl-substituted (21).…”

Section: Resultsmentioning

confidence: 99%

Characterization of supercritical fluid extracts from raw wool by TLC‐FID and GC‐MS

Domínguez

Jover

Garde

et al. 2003

J Americ Oil Chem Soc

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Extraction selectivity of whole wool wax from raw wool by using ethanol-modified carbon dioxide at constant pressure (100 bar) and temperature (60°C) was studied. A kinetic study was carried out to optimize the percentage of ethanol and the extraction time. The modified carbon dioxide was decompressed in ethanol as a collection solvent, leading to the formation of two different fractions as a function of their solubility. Qualitative and quantitative analyses of the lipid classes present in the collected fractions were performed by TLC coupled to an automated FID system (TLC-FID). Moreover, a detailed structural comparison was carried out between the aliphatic high-molecular-mass esters and the steryl ester patterns by means of subambient pressure GC-MS in both the EI and the ammonia positive CI modes. Considerable differences in the lipid composition of the two wool wax fractions collected were observed by TLC-FID and GC-MS.

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“…Chromatography-mass spectrometry combinations are the most efficient techniques nowadays to analyse triacylglycerols [1,2]. The molecular composition of triacylglycerol mixtures is typically very complex due to a combination of a variety of fatty acids, differing in their chain-length, degree of unsaturation and distribution between the sn-1, sn-2 and sn-3 positions of the glycerol backbone.…”

Section: Introductionmentioning

confidence: 99%

Mass spectrometry of triacylglycerols

Laakso

2002

Eur. J. Lipid Sci. Technol.

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Mass spectrometry of complex lipids

Cited by 19 publications

References 181 publications

Dual Analysis of Triglycerides from Certain Common Lipids and Seed Extracts

Dual Analysis of Triglycerides from Certain Common Lipids and Seed Extracts

Characterization of supercritical fluid extracts from raw wool by TLC‐FID and GC‐MS

Mass spectrometry of triacylglycerols

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