Mass Spectrometry Methodology in Lipid Analysis

Abstract

Lipidomics is an emerging field, where the structures, functions and dynamic changes of lipids in cells, tissues or body fluids are investigated. Due to the vital roles of lipids in human physiological and pathological processes, lipidomics is attracting more and more attentions. However, because of the diversity and complexity of lipids, lipid analysis is still full of challenges. The recent development of methods for lipid extraction and analysis and the combination with bioinformatics technology greatly push forward the study of lipidomics. Among them, mass spectrometry (MS) is the most important technology for lipid analysis. In this review, the methodology based on MS for lipid analysis was introduced. It is believed that along with the rapid development of MS and its further applications to lipid analysis, more functional lipids will be identified as biomarkers and therapeutic targets and for the study of the mechanisms of disease.

A Review on Methods Used in Analysis of Microalgae Lipid Composition

“…However, it should be noted that esterification is required for FAs analysis using EI-MS 41) . EI-MS has weak molecular ion signal because of the high energy collision 42) . Thus, CI was developed to produce an intact molecular ion species using lower energy collision of the analyte and ions of a reagent gas.…”

Comparison of Separation of Seed Oil Triglycerides Containing Isomeric Conjugated Octadecatrienoic Acid Moieties by Reversed-Phase HPLC

“…For the same conjugated double bonds position (9,11,13) along the carbon chain, the sequence of elution times is: Pu < Cat < αEl < βEl,…”

“…Mass-spectrometric detection is highly desirable orthogonal method of solute properties control and for seed oils method APCI mass-spectrometry (atmospheric pressure chemical ionization) was developed [13].…”

Combination of ESI and MALDI-MS Imaging Enhances Analysis on Brain Gangliosides

“…MSI has been extensively employed to detect the differentiated pattern of lipids in various organs in different diseases, such as brains in Alzheimer's disease [1,2]. Poor reproducibility of MALDI MSI analysis due to the heterogeneity of the matrix-analyte crystals, hinders its use on quantitative analysis [2]. In addition, discontinuous ion flow due to quickly consumption of the samples under laser irradiation on specific site affects its ability in qualitative analysis.…”

“…The major advantage of MSI is its capability of simultaneously localizing and identifying a parent molecule and its metabolites without any labeling or any prior knowledge. MSI has been extensively employed to detect the differentiated pattern of lipids in various organs in different diseases, such as brains in Alzheimer's disease [1,2]. Poor reproducibility of MALDI MSI analysis due to the heterogeneity of the matrix-analyte crystals, hinders its use on quantitative analysis [2].…”