Mass spectrometry in multi-mycotoxin and fungal spore analysis

Spanjer, M.C.

doi:10.1533/9780857090973.1.90

Cited by 5 publications

(3 citation statements)

References 161 publications

(138 reference statements)

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“…However, tandem mass spectrometry is a powerful tool for the analysis of mycotoxins with high sensitivity and selectivity. A comprehensive review about the analysis of mycotoxins at trace levels by LC-MS/MS is provided by [ 7 ], and an overview of the multi-target methods for the simultaneous determination of mycotoxins in different commodities in recent years is given by [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS

et al. 2012

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A fast, easy-to-handle and cost-effective analytical method for 11 mycotoxins currently regulated in maize and other cereal-based food products in Europe was developed and validated for maize. The method is based on two extraction steps using different acidified acetonitrile–water mixtures. Separation is achieved using ultrahigh-performance liquid chromatography (UHPLC) by a linear water–methanol gradient. After electrospray ionisation, tandem mass spectrometric detection is performed in dynamic multiple reaction monitoring mode. Since accurate mass spectrometric quantification is hampered by matrix effects, uniformly [13C]-labelled mycotoxins for each of the 11 compounds were added to the sample extracts prior to UHPLC-MS/MS analysis. Method performance parameters were obtained by spiking blank maize samples with mycotoxins before as well as after extraction on six levels in triplicates. The twofold extraction led to total recoveries of the extraction steps between 97% and 111% for all target analytes, including fumonisins. The [13C]-labelled internal standards efficiently compensated all matrix effects in electrospray ionisation, leading to apparent recoveries between 88% and 105% with reasonable additional costs. The relative standard deviations of the whole method were between 4% and 11% for all analytes. The trueness of the method was verified by the measurement of several maize test materials with well-characterized concentrations. In conclusion, the developed method is capable of determining all regulated mycotoxins in maize and presuming similar matrix effects and extraction recovery also in other cereal-based foods.

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Section: Introductionmentioning

confidence: 99%

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS

et al. 2012

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“…Further, MS can also distinguish between different food toxins depending upon their mass-to-charge ratio. In order to overcome the limitations associated with the conventional methods, MS can be coupled with these techniques to enhance the capabilities in complete and accurate analysis of different food toxins present in the sample . GC technique relies on the comparison of retention times with known standards and also lacks in the distinction of structurally similar compounds.…”

Section: Recent Advancements In Food Toxin Detection Using Msmentioning

confidence: 99%

“…In order to overcome the limitations associated with the conventional methods, MS can be coupled with these techniques to enhance the capabilities in complete and accurate analysis of different food toxins present in the sample. 98 GC technique relies on the comparison of retention times with known standards and also lacks in the distinction of structurally similar compounds. Thus, to enhance selectivity, identification, and elucidation of the structure of various toxins present in the sample under examination, GC is coupled with the MS. 99 HPLC technique requires optimization such as the selection of columns and mobile phases for each specific class of toxins.…”

Section: Recent Advancements In Food Toxin Detection Using Msmentioning

confidence: 99%

Recent Advances in the Detection of Food Toxins Using Mass Spectrometry

Ahuja,

Singh,

Paul

et al. 2023

Chem. Res. Toxicol.

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Edibles are the only source of nutrients and energy for humans. However, ingredients of edibles have undergone many physicochemical changes during preparation and storage. Aging, hydrolysis, oxidation, and rancidity are some of the major changes that not only change the native flavor, texture, and taste of food but also destroy the nutritive value and jeopardize public health. The major reasons for the production of harmful metabolites, chemicals, and toxins are poor processing, inappropriate storage, and microbial spoilage, which are lethal to consumers. In addition, the emergence of new pollutants has intensified the need for advanced and rapid food analysis techniques to detect such toxins. The issue with the detection of toxins in food samples is the nonvolatile nature and absence of detectable chromophores; hence, normal conventional techniques need additional derivatization. Mass spectrometry (MS) offers high sensitivity, selectivity, and capability to handle complex mixtures, making it an ideal analytical technique for the identification and quantification of food toxins. Recent technological advancements, such as high-resolution MS and tandem mass spectrometry (MS/MS), have significantly improved sensitivity, enabling the detection of food toxins at ultralow levels. Moreover, the emergence of ambient ionization techniques has facilitated rapid in situ analysis of samples with lower time and resources. Despite numerous advantages, the widespread adoption of MS in routine food safety monitoring faces certain challenges such as instrument cost, complexity, data analysis, and standardization of methods. Nevertheless, the continuous advancements in MS-technology and its integration with complementary techniques hold promising prospects for revolutionizing food safety monitoring. This review discusses the application of MS in detecting various food toxins including mycotoxins, marine biotoxins, and plant-derived toxins. It also explores the implementation of untargeted approaches, such as metabolomics and proteomics, for the discovery of novel and emerging food toxins, enhancing our understanding of potential hazards in the food supply chain.

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Keeping mycotoxins away from the food: Does the existence of regulations have any impact in Africa?

Matumba

Poucke

Ediage

et al. 2015

Critical Reviews in Food Science and Nutrition

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Following the discovery of aflatoxins in the early 1960s, there have been many studies leading to the uncovering of many mycotoxins and the understanding of associated health effects in animals and humans. Consequently, there has been a global increase in the number of countries with mycotoxin regulations in foods. However, many African countries have only regulations for aflatoxins (or a few other mycotoxins) in specific foods, or no regulations at all. This paper critically reviews the challenges thwarting the establishment of mycotoxin regulations and their impacts on human dietary mycotoxin exposure in Africa. Mycotoxin regulatory limits for different countries are compared with mycotoxin tolerable daily intakes established by international food safety bodies taking into account consumption patterns. The agrarian setup, food insecurity, and mycotoxin analytical challenges in African countries are discussed; and more feasible mycotoxin dietary exposure reduction strategies are proposed.

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Mass spectrometry in multi-mycotoxin and fungal spore analysis

Cited by 5 publications

References 161 publications

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS

Stable isotope dilution assay for the accurate determination of mycotoxins in maize by UHPLC-MS/MS

Recent Advances in the Detection of Food Toxins Using Mass Spectrometry

Keeping mycotoxins away from the food: Does the existence of regulations have any impact in Africa?

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