Mass Spectrometry Imaging of the Capsaicin Localization in the Capsicum Fruits

Taira, Takunori; Shimma,; Osaka,; Kaneko,; Ichiyanagi,; Ikeda, Kazuhiro; Konishi-Kawamura,; Zhu, Wanxue; Tsuneyama,; Komatsu,

doi:10.6000/1927-3037.2012.01.01.04

Cited by 14 publications

(12 citation statements)

References 18 publications

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“…In contrast, we measured volatiles directly from biological tissues and improved both the technical spatial resolution [10,21], as well as the data visualisation. Capsaicinoids have been imaged before by MALDIToF imaging [37]. Contrary to MALDI-ToF imaging, LTP-MSI does not require special sample preparation prior to measurement and delivers signals of various capsaicinoids at acceptable resolution.…”

Section: Spatial Distribution Of Volatiles and Semi-volatiles In Chilmentioning

confidence: 99%

MSI.R scripts reveal volatile and semi-volatile features in low-temperature plasma mass spectrometry imaging (LTP-MSI) of chilli (Capsicum annuum)

Gamboa-Becerra¹,

Ramı́rez-Chávez²,

Molina‐Torres³

et al. 2015

Anal Bioanal Chem

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In cartography, the combination of colour and contour lines is used to express a three-dimensional landscape on a two-dimensional map. We transferred this concept to the analysis of mass spectrometry imaging (MSI) data and developed a collection of R scripts for the efficient evaluation of .imzML archives in a four-step strategy: (1) calculation of the density distribution of mass-to-charge ratio (m/z) signals in the .imzML file and assembling of a pseudo-master spectrum with peak list, (2) automated generation of mass images for a defined scan range and subsequent visual inspection, (3) visualisation of individual ion distributions and export of relevant .mzML spectra and (4) creation of overlay graphics of ion images and photographies. The use of a Hue-Chroma-Luminance (HCL) colour model in MSI graphics takes into account the human perception for colours and supports the correct evaluation of signal intensities. Further, readers with colour blindness are supported. Contour maps promote the visual recognition of patterns in MSI data, which is particularly useful for noisy data sets. We demonstrate the scalability of MSI.R scripts by running them on different systems: on a personal computer, on Amazon Web Services (AWS) instances and on an institutional cluster. By implementing a parallel computing strategy, the execution speed for .imzML data scanning with image generation could be improved by more than an order of magnitude. Applying our MSI.R scripts ( http://www.bioprocess.org/MSI.R ) to low-temperature plasma (LTP)-MSI data shows the localisation of volatile and semi-volatile compounds in the cross-cut of a chilli (Capsicum annuum) fruit. The subsequent identification of compounds by gas and liquid chromatography coupled to mass spectrometry (GC-MS, LC-MS) proves that LTP-MSI enables the direct measurement of volatile organic compound (VOC) distributions from biological tissues.

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Section: Spatial Distribution Of Volatiles and Semi-volatiles In Chilmentioning

confidence: 99%

MSI.R scripts reveal volatile and semi-volatile features in low-temperature plasma mass spectrometry imaging (LTP-MSI) of chilli (Capsicum annuum)

Gamboa-Becerra¹,

Ramı́rez-Chávez²,

Molina‐Torres³

et al. 2015

Anal Bioanal Chem

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“…MALDI-MS imaging emerged in the medial field, especially in the field of cancer science to gain a better understanding of the metabolite dynamics during tumorigenesis [21], and has also been applied to the food field [22]. In food science, MALDI-MS imaging is now widely used to determine the localization of specific substances of interest such as sugars, amino acids, lipids, and polyphenols [23][24][25], or to screen non-target molecular dynamics in breeding or dosing applications. Thus, MALDI-MS imaging is used not only to clarify the localization of substances in food but also to ensure food safety.…”

Section: History Of Maldi-ms Imaging Applicationsmentioning

confidence: 99%

“…MALDI-MS imaging demonstrated that capsaicin was localized to the surface of the placenta and pericarp but was scarcely present in the seed. From these results, it was considered that capsaicin is likely present in the pericarp to protect the fruit from external enemies, and that a large amount of capsaicin is produced on the surface of the placenta to protect the seed [25].…”

Section: Localization Of Small Metabolites In Foodsmentioning

confidence: 99%

Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging for Food Analysis

Morisasa

Satō

Kimura

et al. 2019

Foods

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Food contains various compounds, and there are many methods available to analyze each of these components. However, the large amounts of low-molecular-weight metabolites in food, such as amino acids, organic acids, vitamins, lipids, and toxins, make it difficult to analyze the spatial distribution of these molecules. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) imaging is a two-dimensional ionization technology that allows the detection of small metabolites in tissue sections without requiring purification, extraction, separation, or labeling. The application of MALDI-MS imaging in food analysis improves the visualization of these compounds to identify not only the nutritional content but also the geographical origin of the food. In this review, we provide an overview of some recent applications of MALDI-MS imaging, demonstrating the advantages and prospects of this technology compared to conventional approaches. Further development and enhancement of MALDI-MS imaging is expected to offer great benefits to consumers, researchers, and food producers with respect to breeding improvement, traceability, the development of value-added foods, and improved safety assessments.

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“…Recently, MSI technique was applied to agricultural and food science fields to reveal plant physiology and localization of nutrition in food body. We have also reported the localization of the nutrition in the food sample [13] and the functional compound in the body after intake [14].…”

Section: Introductionmentioning

confidence: 99%

Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging

Taira¹,

Hashizaki²,

Komori³

et al. 2016

Int. J. Biotech. Well. Indus.

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The use of mass spectrometry imaging (MSI) revealed the localization of-solanine and-chaconine as natural toxins for Potato (Solanum tuberosum L.). The content of Potato glycoalkaloids,-solanine and-chaconine, were quantitatively determined by high performance liquid chromatography (HPLC). Matrix assisted laser desorption/ionization-based tandem mass spectrometry (MS) could determine-solanine and-chaconine from raw potato extraction and section. After budbreak,-solanine and-chaconine were produced and localized at periderm and germ compared with that before budbreak. At germ region, these glycoalkaloids did not exist whole germ region but eccentrically localize at germ surface and central region. The amount of-chaconine was twofold higher than-solanine at periderm. At germ region, there was no difference between these toxins.

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Mass Spectrometry Imaging of the Capsaicin Localization in the Capsicum Fruits

Cited by 14 publications

References 18 publications

MSI.R scripts reveal volatile and semi-volatile features in low-temperature plasma mass spectrometry imaging (LTP-MSI) of chilli (Capsicum annuum)

MSI.R scripts reveal volatile and semi-volatile features in low-temperature plasma mass spectrometry imaging (LTP-MSI) of chilli (Capsicum annuum)

Application of Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry Imaging for Food Analysis

Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging

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