Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging

Taira, Shu; Hashizaki, Riho; Komori, Hanaka; Kazuma, Kohei; Konno, Katsuhiro; Kawabata, Kyuichi; Kaneko, Daisaku; Katano, Hajime

doi:10.6000/1927-3037.2016.05.01.1

Cited by 5 publications

(1 citation statement)

References 14 publications

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“…Each spot irradiated by the laser becomes a pixel in the final image (g). Without the need for target-specific markers such as antibodies, MSI enables simultaneous detection of multiple analytes on a single section of animal tissue , or plant tissue. ,− Recently, MSI has been used to localize ABA in the seeds of Phaseolus vulgaris and to localize ABA and CK ( trans -zeatin, t Z) in rice root …”

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confidence: 99%

Imaging of Multiple Plant Hormones in Roots of Rice (Oryza sativa) Using Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Shiono

Taira

2020

J. Agric. Food Chem.

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Plant hormones can act in synergistic and antagonistic ways in response to biotic and abiotic stresses and in plant growth and development. Thus, a technique is needed to simultaneously determine the distributions and concentrations of several plant hormones. Previously, we reported that localizations of two plant hormones [cytokinin (CK) and abscisic acid (ABA)] can be simultaneously visualized in a plant tissue using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS). In MALDI-MS, however, self-ionization of an organic matrix occasionally interferes with ionizations of small molecules (<500 m/z) including most plant hormones. Another technique, nanoparticle-assisted laser desorption/ionization (Nano-PALDI), can avoid matrix self-ionization using nanoparticles to assist the ionization of analytes. Here, we compared the ionization efficiencies of common plant hormones by MALDI-MS and Nano-PALDI-MS. For the comparison, we prepared a standard mix of seven plant hormones [ABA, auxin (IAA), brassinosteroid (Br), two CKs (trans-zeatin, tZ, and 6-(γ,γ-dimethylallylamino) purine, iP), jasmonic acid, and salicylic acid (SA)], an ethylene precursor (1-aminocyclopropane-1-carboxylic acid, ACC), and a heavy hydrogen-labeled ABA (D6-ABA). Basic MALDI-MS detected all compounds except IAA, Br, and D6-ABA, while Nano-PALDI-MS detected all nine compounds. By Nano-PALDI-MS imaging (MSI), each of the abovementioned hormones and ACC were also detected in root cross sections of rice which were incubated in the hormone mix for 2 h. In the elongation zone of untreated roots, Nano-PALDI-MSI revealed high levels of ABA and CKs in the outer part of roots and much lower levels in the stele, but Br, SA, and ACC were broadly distributed in the cross section. IAA seemed to be distributed in the epidermis, cortex, and stele. Multiple-hormone imaging using Nano-PALDI-MS has great potential for investigating the roles of hormone signaling in crop development and stress responses.

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Section: Introductionmentioning

confidence: 99%

Imaging of Multiple Plant Hormones in Roots of Rice (Oryza sativa) Using Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Shiono

Taira

2020

J. Agric. Food Chem.

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Multi-imaging of Cytokinin and Abscisic Acid on the Roots of Rice (Oryza sativa) Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Shiono

Hashizaki

Nakanishi

et al. 2017

J. Agric. Food Chem.

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Plant hormones act as important signaling molecules that regulate responses to abiotic stress as well as plant growth and development. Because their concentrations of hormones control the physiological responses in the target tissue, it is important to know the distributions and concentrations in the tissues. However, it is difficult to determine the hormone concentration on the plant tissue as a result of the limitations of conventional methods. Here, we report the first multi-imaging of two plant hormones, one of cytokinin [i.e., trans-zeatin (tZ)] and abscisic acid (ABA) using a new technology, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) imaging. Protonated signals of tZ (m/z 220.1) and ABA (m/z 265.3) were chosen on longitudinal sections of rice roots for MS imaging. tZ was broadly distributed about 40 mm behind the root apex but was barely detectable at the apex, whereas ABA was mainly detected at the root apex. Multi-imaging using MALDI-TOF-MS enabled the visualization of the localization and quantification of plant hormones. Thus, this tool is applicable to a wide range of plant species growing under various environmental conditions.

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Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration

Tatsuta

Kasai

Maruyama

et al. 2017

Sci Rep

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We analyzed the localization of ubiquinol, the reduced form of coenzyme Q10 (Re-CoQ10), in mouse brain sections using matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance imaging mass spectrometry (IMS) to evaluate the effect of dietary Re-CoQ10 in mouse brain. Mice were orally administered Re-CoQ10 for 14 days and brain Re-CoQ10 content was subsequently quantified using liquid chromatography-mass spectrometry. IMS was employed to visualize Re-CoQ10 at a resolution of 150 μm in the mouse brain. Increased Re-CoQ10 was observed in the corpus callosum, hippocampus, midbrain, cerebellum, brain stem, substantia nigra and striatum. These regions are related to movement, memory and vital life functions. Thus, we demonstrated the effect of Re-CoQ10 administration on the specific localization of Re-CoQ10 in the brain.

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Localization Analysis of Natural Toxin of Solanum tuberosum L. via Mass Spectrometric Imaging

Cited by 5 publications

References 14 publications

Imaging of Multiple Plant Hormones in Roots of Rice (Oryza sativa) Using Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Imaging of Multiple Plant Hormones in Roots of Rice (Oryza sativa) Using Nanoparticle-Assisted Laser Desorption/Ionization Mass Spectrometry

Multi-imaging of Cytokinin and Abscisic Acid on the Roots of Rice (Oryza sativa) Using Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry

Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration

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