Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration

Tatsuta, Yukina; Kasai, Kazuhiko; Maruyama, Chitose; Hamano, Yoshimitsu; Matsuo, Keitaro; Katano, Hajime; Taira, Shu

doi:10.1038/s41598-017-13257-8

Cited by 22 publications

(21 citation statements)

References 29 publications

(28 reference statements)

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“…4. While the protonated forms of oxidised and reduced coenzyme Q9 and Q10 have been detected using MALDI-MSI from brain tissue [39], to our knowledge, this is the first report of their detection in renal tissue with MSI.…”

Section: High Spatial Resolution Imaging Of Lipids In Kidney Tissuementioning

confidence: 78%

Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation

et al. 2019

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Matrix-assisted laser desorption/ionisation-mass spectrometry imaging (MALDI-MSI) is a powerful technique for visualising the spatial locations of lipids in biological tissues. However, a major challenge in interpreting the biological significance of local lipid compositions and distributions detected using MALDI-MSI is the difficulty in associating spectra with cellular lipid metabolism within the tissue. By-and-large this is due to the typically limited spatial resolution of MALDI-MSI (30-100 μm) meaning individual spectra represent the average spectrum acquired from multiple adjacent cells, each potentially possessing a unique lipid composition and biological function. The use of oversampling is one promising approach to decrease the sampling area and improve the spatial resolution in MALDI-MSI, but it can suffer from a dramatically decreased sensitivity. In this work we overcome these challenges through the coupling of oversampling MALDI-MSI with laser post-ionisation (MALDI-2). We demonstrate the ability to acquire rich lipid spectra from pixels as small as 6 μm, equivalent to or smaller than the size of typical mammalian cells. Coupled with an approach for automated lipid identification, it is shown that MALDI-2 combined with oversampling at 6 μm pixel size can detect up to three times more lipids and many more lipid classes than even conventional MALDI at 20 μm resolution in the positive-ion mode. Applying this to mouse kidney and human brain tissue containing active multiple sclerosis lesions, where 74 and 147 unique lipids are identified, respectively, the localisation of lipid signals to individual tubuli within the kidney and lipid droplets with lesion-specific macrophages is demonstrated.

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Section: High Spatial Resolution Imaging Of Lipids In Kidney Tissuementioning

confidence: 78%

Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation

et al. 2019

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“…Some examples of this application include the localisation of dietary substrates in the brain or lipid changes in the brain after traumatic injury. 218,219 Studies of cells have also been done with IMS. As with tissue, lipid changes have been observed in both neuronal cell cultures and PC12 cells.…”

Section: Applications For Studying Cellular Signalling and Exocytosismentioning

confidence: 99%

Extracellular ATP Regulates the Vesicular Pore Opening in Chromaffin Cells and Increases the Fraction Released During Individual Exocytosis Events

Majdi

Larsson

Najafinobar

et al. 2019

ACS Chem. Neurosci.

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Cellular communication is a prerequisite in multicellular organisms in order to survive. Many times, this communication occurs through the highly controlled and regulated release of chemical signals through a process known as exocytosis. This process consists of organelles known as vesicles fusing with the plasma membrane to release the content inside to the extracellular space. Although the basic underpinnings of this process are known, exactly how it is regulated is still largely unknown.This thesis covers studies performed on mammalian cell lines and invertebrate neurons with the aim to further understand regulation of exocytosis. Several IV

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“…The image of protonated Gly-PA was blurred around the crushed oil glands but PA was nonetheless clearly localized at oil glands. A small amount of Gly-PA was observed in the epidermis region (Figure 3c, d (Taira, 2008;Tatsuta, 2017), and may have applications in agriculture relevant to commercial food products.…”

Section: Maldi-msi Of Perilla Herb Sectionsmentioning

confidence: 99%

Localization analysis of essential oils in perilla herb (Perilla frutescens var. crispa) using derivatized mass spectrometry imaging

Taira

Kiriake-Yoshinaga

Shikano

et al. 2021

Food Science & Nutrition

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Perilla herb (Perilla frutescens var. crispa) is an annual plant that grows wild and is cultivated for food in many regions in Asia. Aromatic herbs such as perilla contain essential oils such as monoterpenoid perillaldehyde (PA) and bicyclic sesquiterpenes β-caryophyllene (βC) and have distinctive fragrances. PA and βC also exhibit antibacterial and local anesthetic activity. Perilla herb is thus used as a herbal medicine component (Legault & Pichette, 2007) and may be an antidote for food poisoning. Rosmarinic acid (RA), a common component in perilla herb, has anti-inflammatory and antioxidant activity, enhances the concentration of monoamine in the brain, and inhibits amyloidβ aggregation (Hase, 2019). These essential oils act as secondary metabolites in extracellular oil glands in perilla (Figure 1). The oils are produced in inner plant cells and are transported to and stored in the oil glands. Conventional staining methods using Sudan red show the presence of essential oils in oil glands but provides only indirect information as it does not

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Imaging mass spectrometry analysis of ubiquinol localization in the mouse brain following short-term administration

Cited by 22 publications

References 29 publications

Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation

Evaluation of lipid coverage and high spatial resolution MALDI-imaging capabilities of oversampling combined with laser post-ionisation

Extracellular ATP Regulates the Vesicular Pore Opening in Chromaffin Cells and Increases the Fraction Released During Individual Exocytosis Events

Localization analysis of essential oils in perilla herb (Perilla frutescens var. crispa) using derivatized mass spectrometry imaging

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