Mass Spectrometry Imaging of Bio‐oligomer Polydispersity in Plant Tissues by Laser Desorption Ionization from Silicon Nanopost Arrays

Abstract

Mass spectrometry imaging (MSI) enables simultaneous spatial mapping for diverse molecules in biological tissues.M atrix-assisted laser desorption ionization (MALDI) mass spectrometry (MS) has been am ainstream MSI method for aw ide range of biomolecules.H owever,M ALDI-MSI of biological homopolymers used for energy storage and molecular feedstocki sl imited by,e .g.,p referential ionization for certain molecular classes.Matrix-free nanophotonic ionization from silicon nanopost arrays (NAPAs) is an emerging laser desorption ionization (LDI) platform with ultra-trace sensitivity and molecular imaging capabilities.H ere,w es how complementary analysis and MSI of polyhydroxybutyric acid (PHB), polyglutamic acid (PGA), and polysaccharide oligomers in soybean root nodule sections by NAPA-LDI and MALDI. ForP HB,n umber and weight average molar mass, polydispersity,and oligomer sizedistributions across the tissue section and in regions of interest were characterized by NAPA-LDI-MSI.

Spatial Mapping of Plant N-Glycosylation Cellular Heterogeneity Inside Soybean Root Nodules Provided Insights Into Legume-Rhizobia Symbiosis

“…These specialized root organs have several visually and functionally distinct compartments (i.e., outer cortex, sclerenchyma, inner cortex, and infection zone), where a population of root cells is infected with nitrogen-fixing soil bacteria (Rhizobiaceae) (Livingston et al, 2019 ). Using the MALDI-MSI (Velickovic et al, 2018 ) and other MSI modalities (Agtuca et al, 2020 ; Samarah et al, 2021 ), we recently spatially resolved critical metabolic and lipidomic pathways in different compartments of soybean root nodules, and also showed different metabolic profiles in infected and uninfected plant cells inside the infection zone (Samarah et al, 2020 ).…”

Spatially resolved metabolomics reveals variety-specific metabolic changes in banana pulp during postharvest senescence

“…To circumvent these issues, MS imaging (MSI) techniques have been used as a label-free and non-specific detection tool to provide the spatiotemporal distribution information on a broad range of metabolites in biological tissues ( Li et al, 2021 , Yin et al, 2019 ). In recent years, several efforts have been made to develop matrix-assisted laser desorption/ionization (MALDI)-MSI technologies for mapping metabolite abundances in various fruits and vegetables, such as strawberry and apple fruits ( Horikawa et al, 2019 ), soybean root nodules ( Samarah et al, 2021 ), and tomato fruits ( Dong et al, 2020 ). In addition, Hölscher et al used 1 H NMR spectroscopy, laser desorption/ionization (LDI)-MSI, and Raman spectroscopy to identify and locate phenylphenalenones, a banana-specific type of phytoalexins, within the Musa spp.…”

Nanoscale Three-Dimensional Imaging of Drug Distributions in Single Cells via Laser Desorption Post-Ionization Mass Spectrometry

“…■ CONCLUSIONS 3D-MSI has been considered to be a crucial technology that can provide more comprehensive information in life sciences research. In this work, 3D-MSI information for two anticancer drugs within single cells is obtained at a 500 × 500 × 500 nm 3 voxel resolution with the MLF-LDPI-TOFMS technique.…”

“…Mass spectrometry imaging (MSI) is a label-free and nonspecific molecular imaging technique that enables the visualization of molecular distributions on a sample surface. − It has been extensively used in the field of biomedical and material science research, especially in life science studies. − Most MSI studies are conducted at the two-dimensional (2D) level. A chemical snapshot of the sample surface is obtained, which corresponds to the specific molecular location on the surface.…”