Mass spectrometry images acylcarnitines, phosphatidylcholines, and sphingomyelin in MDA-MB-231 breast tumor models

Detection and confirmation of serum lipid biomarkers for preeclampsia using direct infusion mass spectrometry

“…Again, all fragments were exactly as predicted and no other fragments were found. Studies involving identifi cation of PCs based on the neutral losses of the fatty acyl chains are commonly observed in literature (33)(34)(35). Collectively, our study and otherwise commonly observed in the MS 2 spectrum of PCs.…”

“…The fragmentation spectrum of the marker with m/z 734.6 also displayed a low abundance fragment ion with m/z 496.3, suggesting the presence of 16:0 fatty acyl chain ( 33 ) ( Fig. 6 ).…”

Molecular anatomy of ascending aorta in atherosclerosis by MS Imaging: Specific lipid and protein patterns reflect pathology

“…For identity assignment, tissue samples were also analyzed on a 9.4T Solarix FT-ICR (BrukerDaltonik, Bremen, Germany) mass spectrometer. Lipid molecular identification was performed by using exact mass measurements, peak peaking and spatial filtering combined with Lipidsmap database using a tolerance of ≤ 0.005Da, as previously published [26,27].…”

Oxidative Lipidomics Coming of Age: Advances in Analysis of Oxidized Phospholipids in Physiology and Pathology

“…Ion mobility separates ions based on their shape (more correctly on their average collisional crosssection) and has proved particularly useful in lipidomics for distinguishing isobaric lipids from different classes or of different double bond configurations (2,14,56,66,69,93,109). It also has great potential for removing interfering chemical background from the mass spectra, allowing better assignment and more sensitive detection, and has proved particularly useful in mass spectrometric imaging applications (16,52,144). This technique has not yet been applied specifically to oxidized lipid species, although there are some reports that include oxidized lipids (51), but it will have great potential for adding an additional dimension of separation.…”