Detection and confirmation of serum lipid biomarkers for preeclampsia using direct infusion mass spectrometry

Anand, Swati; Young, Sydney A; Esplin, Sean; Peaden, Benjamin; Tolley, H. Dennis; Porter, T. Flint; Varner, Michael W.; D’Alton, Mary E.; Jackson, Bruce J.; Graves, Steven W.

doi:10.1194/jlr.p064451

Cited by 67 publications

(60 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Lipids with an m/z of 383 have been identified as oxidized cholesterol, which has been shown to contribute to vascular atherosclerosis in PE. 76 However, lipids with an m/z of 784, 796, 798, and 920 belong to the glycerophosphocholine-lipid class, which may be elevated in PE pregnancies due to enhanced cellular apoptosis. 76 Although lipidomics provide a new avenue for the identification of PE biomarkers, further studies are needed to determine if these markers are specific for PE.…”

Section: Lipidsmentioning

confidence: 99%

Placenta-derived exosomes: potential biomarkers of preeclampsia

Pillay¹,

Moodley²,

Moodley³

et al. 2017

IJN

107

View full text Add to dashboard Cite

Preeclampsia remains a leading cause of maternal and fetal mortality, due to ineffective treatment and diagnostic strategies, compounded by the lack of clarity on the etiology of the disorder. Although several clinical and biological markers of preeclampsia have been evaluated, they have proven to be ineffective in providing a definitive diagnosis during the various stages of the disorder. Exosomes have emerged as ideal biomarkers of pathological states, such as cancer, and have more recently gained interest in pregnancy-related complications, due to their role in cellular communication in normal and complicated pregnancies. This occurs as a result of the specific placenta-derived exosomal molecular cargo, which may be involved in normal pregnancy-associated immunological events, such as the maintenance of maternal–fetal tolerance. This review provides perspectives on placenta-derived exosomes as possible biomarkers for the diagnosis/prognosis of preeclampsia. Using keywords, online databases were searched to identify relevant publications to review the potential use of placenta-derived exosomes as biomarkers of preeclampsia.

show abstract

Section: Lipidsmentioning

confidence: 99%

Placenta-derived exosomes: potential biomarkers of preeclampsia

Pillay¹,

Moodley²,

Moodley³

et al. 2017

IJN

107

View full text Add to dashboard Cite

show abstract

“…In this scenario, considering that, among other pathological alterations, neurodegeneration involves also the breakdown of cell membranes to result in abnormal abundances of membrane lipids, MS has been widely applied to determine lipid biomarkers that could discriminate between AD and control patients . Although the contribution of MS to lipidomics is vast, the main application of MS in the AD field is the analysis of lipids extracted from biological fluids with either direct infusion or with an HPLC step in order to improve sensitivity and separation of the different lipidic components .…”

Section: Beyond the Aβ Peptides: The Contribution Of Mass Spectrometrmentioning

confidence: 99%

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Grasso

2018

Mass Spectrometry Reviews

View full text Add to dashboard Cite

Amyloid-β peptide (Aβ) accumulation and aggregation have been considered for many years the main cause of Alzheimer's disease (AD), and therefore have been the principal target of investigation as well as of the proposed therapeutic approaches (Grasso [2011] Mass Spectrom Rev. 30: 347-365). However, the amyloid cascade hypothesis, which considers Aβ accumulation the only causative agent of the disease, has proven to be incomplete if not wrong. In recent years, actors such as metal ions, oxidative stress, and other cofactors have been proposed as possible co-agents or, in some cases, main causative factors of AD. In this scenario, MS investigation has proven to be fundamental to design possible diagnostic strategies of this elusive disease, as well as to understand the biomolecular mechanisms involved, in the attempt to find a possible therapeutic solution. We review the current applications of MS in the search for possible Aβ biomarkers of AD to help the diagnosis of the disease. Recent examples of the important contributions that MS has given to prove or build theories on the molecular pathways involved with such terrible disease are also reviewed.

show abstract

“…Furthermore, a lack of glycerophospholipid catabolism is also “telltale” in certain pathophysiologies as irregular PC metabolism has been implicated in breast and ovarian tumor progression (69). For example, the PC species, PC 36:4, was also reported as an early lipid biomarker for the development of pre-eclampsia, which suggested that a lack of PC hydrolysis and subsequent generation of eicosanoids is an indicator of an inability of the body to resolve certain inflammatory disorders (70). Unpublished and preliminary findings by our laboratory also support this premise and implicate both increased levels of PC with arachidonate in the sn 2 position and decreased levels of LPC as early biomarkers of a poorer outcome for sepsis patients (Fig.…”

Section: The Roles Of Various Lipid Classes In Disease Pathophysiologiesmentioning

confidence: 99%

Lipidomics in translational research and the clinical significance of lipid-based biomarkers

Stephenson

Hoeferlin

Chalfant

2017

Translational Research

103

View full text Add to dashboard Cite

Lipidomics is a rapidly developing field of study that focuses on the identification and quantitation of various lipid species in the lipidome. Lipidomics has now emerged in the forefront of scientific research due to the importance of lipids in metabolism, cancer, and disease. Using both targeted and untargeted mass spectrometry as a tool for analysis, progress in the field has rapidly progressed in the last decade. Having the ability to assess these small molecules in vivo has led to better understanding of several lipid-driven mechanisms and the identification of lipid-based biomarkers in neurodegenerative disease, cancer, sepsis, wound healing, and pre-eclampsia. Biomarker identification and mechanistic understanding of specific lipid pathways linked to a disease pathologies can form the foundation in the development of novel therapeutics in hopes of curing human disease.

show abstract

Detection and confirmation of serum lipid biomarkers for preeclampsia using direct infusion mass spectrometry

Cited by 67 publications

References 52 publications

Placenta-derived exosomes: potential biomarkers of preeclampsia

Placenta-derived exosomes: potential biomarkers of preeclampsia

Mass spectrometry is a multifaceted weapon to be used in the battle against Alzheimer's disease: Amyloid beta peptides and beyond

Lipidomics in translational research and the clinical significance of lipid-based biomarkers

Contact Info

Product

Resources

About