Mass Spectrometry Identification of Biomarkers in Extracellular Vesicles From Plasmodium vivax Liver Hypnozoite Infections

Gualdrón‐López, Melisa; Díaz-Varela, Míriam; Zanghì, Gigliola; Aparici-Herraiz, Iris; Steel, Ryan; Schäfer, Carola; Cuscó, Pol; Chuenchob, Vorada; Kangwangransan, Niwat; Billman, Zachary P.; Olsen, Tayla M.; González, Juan R.; Roobsoong, Wanlapa; Sattabongkot, Jetsumon; Murphy, Sean C.; Mikolajczak, Sebastian A.; Borràs, Eva; Sabidó, Eduard; Fernández-Becerra, Carmen; Flannery, Erika L.; Kappe, Stefan H. I.; Portillo, Hernando A. del

doi:10.1016/j.mcpro.2022.100406

Cited by 9 publications

(6 citation statements)

References 56 publications

(78 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, studies involving liver‐humanized mouse models, which can sustain P. vivax infections, have enabled the study of Pv EVs. Thus, using this liver humanized mouse model bearing P. vivax pre‐erythrocytic stages, parasite proteins were identified in EVs coming from plasma of these infected liver humanized mouse model, which represents new avenues for the research of biomarkers for vivax liver infection, including hypnozoites (Gualdron‐Lopez et al., 2022, 2018). In contrast to previous studies, EVs were purified by SEC using 300–500 μL of plasma from malaria‐infected liver‐chimeric humanized mice.…”

Section: Specific Methodologies For Isolation Characterization and Pu...mentioning

confidence: 99%

“…It can also fractionate different EVs populations that can be further recognized by the presence of specific antigens (Ribeiro et al., 2018; Trocoli Torrecilhas et al., 2009). In malaria, has been recently used to purified EVs directly from plasma of P. vivax ‐infected patients or from liver‐humanized mouse models (Gualdron‐Lopez et al., 2018, 2022; Toda et al., 2020).…”

Section: Specific Methodologies For Isolation Characterization and Pu...mentioning

confidence: 99%

“…To extrapolate these data to human infections, it was later shown that reticulocyte-derived exosomes obtained from in vitro cultures of human reticulocytes contain MHC class I antigens and were specifically uptake by mature dendritic cells (Diaz-Varela et al, 2018). In addition, immunocapture of circulating EVs in natural P. vivax infections using antibodies against the transferrin receptor (CD71) followed by mass spectrometry identified novel antigens for vaccination (Aparici Herraiz et al, 2022). Altogether, these data support the research and development of EVs as a novel vaccination approach against malaria.…”

Section:  Parasite Evs and Vaccinesmentioning

confidence: 99%

See 2 more Smart Citations

Guidelines for the purification and characterization of extracellular vesicles of parasites

Fernandez‐Becerra,

Xander,

Alfandari

et al. 2023

J of Extracellular Bio

Self Cite

View full text Add to dashboard Cite

Parasites are responsible for the most neglected tropical diseases, affecting over a billion people worldwide (WHO, 2015) and accounting for billions of cases a year and responsible for several millions of deaths. Research on extracellular vesicles (EVs) has increased in recent years and demonstrated that EVs shed by pathogenic parasites interact with host cells playing an important role in the parasite's survival, such as facilitation of infection, immunomodulation, parasite adaptation to the host environment and the transfer of drug resistance factors. Thus, EVs released by parasites mediate parasite‐parasite and parasite‐host intercellular communication. In addition, they are being explored as biomarkers of asymptomatic infections and disease prognosis after drug treatment. However, most current protocols used for the isolation, size determination, quantification and characterization of molecular cargo of EVs lack greater rigor, standardization, and adequate quality controls to certify the enrichment or purity of the ensuing bioproducts. We are now initiating major guidelines based on the evolution of collective knowledge in recent years. The main points covered in this position paper are methods for the isolation and molecular characterization of EVs obtained from parasite‐infected cell cultures, experimental animals, and patients. The guideline also includes a discussion of suggested protocols and functional assays in host cells

show abstract

Section: Specific Methodologies For Isolation Characterization and Pu...mentioning

confidence: 99%

Section: Specific Methodologies For Isolation Characterization and Pu...mentioning

confidence: 99%

Section:  Parasite Evs and Vaccinesmentioning

confidence: 99%

See 1 more Smart Citation

Guidelines for the purification and characterization of extracellular vesicles of parasites

Fernandez‐Becerra,

Xander,

Alfandari

et al. 2023

J of Extracellular Bio

Self Cite

View full text Add to dashboard Cite

show abstract

“…Based on literature research, we found that mass spectrometry is currently the most commonly used method for proteomic analysis of BEVs, including pathogenicity and immune response of pathogens, 200 antibiotic resistance of pathogens, 201 etc. In addition, other techniques are available for proteomic analysis of tumor‐derived EVs, 202 and circular EVs, 203 such as Aptamer, Proximity extension assays (PEA), Low density array (LDA) analysis, and Immunoaffinity assays. These methods may also be applicable to BEVs proteomic analysis.…”

Section: Recommendations and Considerationsmentioning

confidence: 99%

Bacterial extracellular vesicles: A position paper by the microbial vesicles task force of the Chinese society for extracellular vesicles

Wen

Wang

et al. 2023

Interdisciplinary Medicine

View full text Add to dashboard Cite

Recently, the interest in extracellular vesicles released by bacteria has rapidly increased. Bacterial extracellular vesicles (BEVs) have been involved in bacteria‐bacteria and bacteria‐host interactions, which strengthen health or bring about various pathologies. However, BEV separation, characterization, and functional studies require the establishment of guidelines and further optimization in order to stimulate the development of science in BEV research and a following successful transformation into clinical applications. This position paper is authored by the Microbial Vesicles Task Force of the Chinese Society for Extracellular Vesicles (CSEV) composed of experienced medical laboratory specialists, microbiologists, virologists, biologists and material biologists who are actively engaged in BEV research. Herein, we present a concise description of BEV research and discover challenges and critical gaps in current BEV‐based analyses for clinical applications. Finally, we also offer suggestions and considerations to improve experimental reproducibility and interoperability in BEV research to promote progress in the field.

show abstract

“…In this context, EVs are a resource with great potential [ 2 ], as these small lipidic vesicles of cellular origin are present in all biological fluids and have great stability. In fact, the potential of EVs as a source of disease-relevant biomarkers has been proposed for other parasitic pathogens [ 14 , 15 , 16 , 17 , 18 ]. EVs from Leishmania have been previously characterized [ 19 , 20 ] and parasite-associated proteins were also identified in infected macrophages [ 21 , 22 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization and Proteomic Analysis of Plasma EVs Recovered from Healthy and Diseased Dogs with Canine Leishmaniosis

Lima

Costa

Osório

et al. 2023

IJMS

Self Cite

View full text Add to dashboard Cite

Dogs are highly valued companions and work animals that are susceptible to many life-threatening conditions such as canine leishmaniosis (CanL). Plasma-derived extracellular vesicles (EVs), exploited extensively in biomarker discovery, constitute a mostly untapped resource in veterinary sciences. Thus, the definition of proteins associated with plasma EVs recovered from healthy and diseased dogs with a relevant pathogen would be important for biomarker development. For this, we recovered, using size-exclusion chromatography (SEC), EVs from 19 healthy and 20 CanL dogs’ plasma and performed proteomic analysis by LC-MS/MS to define their core proteomic composition and search for CanL-associated alterations. EVs-specific markers were identified in all preparations and also non-EVs proteins. Some EVs markers such as CD82 were specific to the healthy animals, while others, such as the Integrin beta 3 were identified in most samples. The EVs-enriched preparations allowed the identification of 529 canine proteins that were identified in both groups, while 465 and 154 were only identified in healthy or CanL samples, respectively. A GO enrichment analysis revealed few CanL-specific terms. Leishmania spp. protein identifications were also found, although with only one unique peptide. Ultimately, CanL-associated proteins of interest were identified and a core proteome was revealed that will be available for intra- and inter-species comparisons.

show abstract

Mass Spectrometry Identification of Biomarkers in Extracellular Vesicles From Plasmodium vivax Liver Hypnozoite Infections

Cited by 9 publications

References 56 publications

Guidelines for the purification and characterization of extracellular vesicles of parasites

Guidelines for the purification and characterization of extracellular vesicles of parasites

Bacterial extracellular vesicles: A position paper by the microbial vesicles task force of the Chinese society for extracellular vesicles

Characterization and Proteomic Analysis of Plasma EVs Recovered from Healthy and Diseased Dogs with Canine Leishmaniosis

Contact Info

Product

Resources

About