Bacterial extracellular vesicles: A position paper by the microbial vesicles task force of the Chinese society for extracellular vesicles

Abstract

Recently, the interest in extracellular vesicles released by bacteria has rapidly increased. Bacterial extracellular vesicles (BEVs) have been involved in bacteria‐bacteria and bacteria‐host interactions, which strengthen health or bring about various pathologies. However, BEV separation, characterization, and functional studies require the establishment of guidelines and further optimization in order to stimulate the development of science in BEV research and a following successful transformation into clinical applications. This position paper is authored by the Microbial Vesicles Task Force of the Chinese Society for Extracellular Vesicles (CSEV) composed of experienced medical laboratory specialists, microbiologists, virologists, biologists and material biologists who are actively engaged in BEV research. Herein, we present a concise description of BEV research and discover challenges and critical gaps in current BEV‐based analyses for clinical applications. Finally, we also offer suggestions and considerations to improve experimental reproducibility and interoperability in BEV research to promote progress in the field.

Single‐particle analysis of circulating bacterial extracellular vesicles reveals their biogenesis, changes in blood and links to intestinal barrier

“…Identification of the components released by the bacteria is a key approach to investigate their specific functions. Among the substances secreted by bacteria, bacterial extracellular vesicles (BEVs) have drawn substantial attention due to their unique roles (Erttmann et al, 2022;Wen et al, 2023). BEVs are nanoscale vesicles (20-250 nm) with lipid bilayer that are naturally released by bacteria (Kaparakis-Liaskos & Ferrero, 2015;Toyofuku et al, 2019).…”

The prognosis and immune repertoire characteristics of HBsAg and anti-HBs double positivity chronic hepatitis B patients

“…It is crucial to recruit DP group with different responses to IFN-α treatment to explore underlying mechanisms and identify therapeutic targets. Furthermore, integrating immune repertoire sequencing with single-cell sequencing technology or other advanced technologies holds promise for a deeper understanding of DP pathogenesis [37,41,42] .…”

Harnessing Bacterial Extracellular Vesicle Immune Effects for Cancer Therapy

“…Despite these early promising results, there exist several significant hurdles in the transition of bEVs from bench-to-bedside clinical applications, some of which were summarized in position papers of the International Society for Extracellular Vesicles [ 145 ] and Chinese Society for Extracellular Vesicles [ 146 ]. These concerns include toxicity of bacterial components, consistency of purification, and cost of production.…”