Mass Spectrometry-Based Metabolomic and Lipidomic Analysis of the Effect of High Fat/High Sugar Diet and GreenshellTM Mussel Feeding on Plasma of Ovariectomized Rats

Abstract

This study aimed to examine the changes in lipid and metabolite profiles of ovariectomized (OVX) rats with diet-induced metabolic syndrome-associated osteoarthritis (MetOA) after supplementation with greenshell mussel (GSM) using an untargeted liquid chromatography-mass spectrometry (LC-MS) metabolomics approach. Ninety-six rats were fed with one of four diets: control, control supplemented with GSM + GSM, high fat/high sugar (HFHS), or high fat/high sugar enriched with GSM (HFHS + GSM). After 8 weeks on experimental diets, half of the rats in each group underwent OVX and the other half were sham operated. After being fed for an additional 28 weeks, blood samples were collected for the metabolomics analysis. Lipid and polar metabolites were extracted from plasma and analysed by LC-MS. We identified 29 lipid species from four lipid subclasses (phosphatidylcholine, lysophosphatidylcholine, diacylglycerol, and triacylglycerol) and a set of eight metabolites involved in amino acid metabolism (serine, threonine, lysine, valine, histidine, pipecolic acid, 3-methylcytidine, and cholic acid) as potential biomarkers for the effect of HFHS diet and GSM supplementation. GSM incorporation more specifically in the control diet generated significant alterations in the levels of several lipids and metabolites. Further studies are required to validate these findings that identify potential biomarkers to follow OA progression and to monitor the impact of GSM supplementation.

Phenolamide extract of apricot bee pollen alleviates glucolipid metabolic disorders and modulates the gut microbiota and metabolites in high-fat diet-induced obese mice

“…10 A growing number of studies are using metabolomics for biological validation, dissection of disease metabolism, and monitoring of intervention effects. 11,12 Gut metabolites identified by metabolomics include those derived from the host gut microbiota and transformed from the interaction of active ingredients with the host gut microbiota, which are transported throughout the body. 13 Previous studies have reported a significant difference in metabolites between HFD-fed mice and lean mice.…”

Utilising A Clinical Metabolomics LC-MS Study to Determine the Integrity of Biological Samples for Statistical Modelling after Long Term −80 °C Storage: A TOFI_Asia Sub-Study

“…Although our study compared features derived from different methods of extraction (bi-vs. monophasic extraction) and means of acquisition (Orbitrap vs. TOF mass spectrometry), previous reports [55][56][57] along with our in-house optimizing [58] strongly indicated that a change from biphasic to monophasic extraction protocols for lipids and polar metabolites would yield comparable features for the current study outcomes. Furthermore, the metabo-RING study, which evaluated the reliability of untargeted metabolomics data across various laboratories with their respective LCMS instruments and in-house extraction protocols, reported comparable results from the same urine and plasma samples [32].…”

Correlation between body condition score and body composition in a rat model for obesity research

“…This model has been frequently used in obesity research as the convenience of the protocol and representing the public health problem. The surgical model using ovariectomy in female subjects can mimic the menopause condition in women as estrogen loss induces increased food consumption at the transitional period (Siriarchavatana et al, 2022) and worsens metabolic and lipid profiles (Siriarchavatana et al, 2020;Abshirini et al, 2021). Boonyapakorn et al (2019) demonstrated that lacks of testosterone in castrated dogs resulted in significant weight gain due to the reduction of physical activities but this manipulation did not affect metabolic profiles or cardiac function.…”