Mass Spectrometry-Based Metabolite Profiling and Antioxidant Activity of Aloe vera (Aloe barbadensis Miller) in Different Growth Stages

Abstract

Metabolite profiling of four different-sized Aloe vera plants was performed using gas chromatography-ion trap-mass spectrometry (GC-IT-MS) and ultra performance liquid chromatography-quadrupole-time of flight-mass spectrometry (UPLC-Q-TOF-MS) with multivariate analysis. Amino acids, sugars, and organic acids related to growth and development were identified by sizes. In particular, the relative contents of glucose, fructose, alanine, valine, and aspartic acid increased gradually as the size of the aloe increased. Anthraquinone derivatives such as 7-hydroxy-8-O-methylaloin, 7-hydroxyaloin A, and 6'-malonylnataloins A and B increased gradually, whereas chromone derivatives decreased continuously as the size of the aloe increased. The A30 aloe (size = 20-30 cm) with relatively high contents of aloins A and B, was suggested to have antioxidant components showing the highest antioxidant activity among the four different sizes of aloe. These data suggested that MS-based metabolomic approaches can illuminate metabolite changes associated with growth and development and can explain their change of antioxidant activity.

Metabolomics of Lonicera caerulea fruit during ripening and its relationship with color and antioxidant activity

“…Flavonoids (such as quercetin, and kaempferol) are synthesized in all plants (Lee et al, 2012;Toffali et al, 2011). Quercetin-3-glucoside and quercetin-3-rutinoside are the most abundant flavonoids in L. caerulea fruits (Kusznierewicz et al, 2012;Ren et al, 2008).…”

De-novo RNA Sequencing and Metabolite Profiling to Identify Genes Involved in Anthocyanin Biosynthesis in Korean Black Raspberry (Rubus coreanus Miquel)

“…In LC-MS analysis, the accurate masses and elemental compositions were calculated using the MassLynx software (Waters Corp.) incorporated in UPLC-Q-TOF-MS, MS n fragmentation patterns and UV spectrum were also supported to identify major metabolites using MS workstation software (version 6.9, Varian, USA) in LC-IT-MS/MS. When standard compounds were not available, a tentative identification was performed based on the MS spectra using the NIST05 MS Library (NIST, 2005), combined chemical dictionary version 7.2 (Chapman & Hall/CRC), references, and an in-house library [33], [34]. The metabolomics standards initiative (MSI) has been categorized into four different levels according to [35].…”

Chemotaxonomic Metabolite Profiling of 62 Indigenous Plant Species and Its Correlation with Bioactivities

“…Liquid chromatography-mass spectrometry (LC-MS)-based metabolite profiling is valuable for analysis of compounds over a wide range of polarity and molecular weight [26]. Beneficial secondary compounds in plants, such as flavonoids, phenolic compounds, and terpenoids have been identified [27,28], and plant bioactivities, including antioxidant activity [29], antimicrobial activity, and tyrosinase inhibition activity [30] have been reported. However, few studies have attempted to reveal the relationship between metabolite differences and bioactivity in diverse plant species.…”