Chemotaxonomic Metabolite Profiling of 62 Indigenous Plant Species and Its Correlation with Bioactivities

Abstract

Chemotaxonomic metabolite profiling of 62 indigenous Korean plant species was performed by ultrahigh performance liquid chromatography (UHPLC)-linear trap quadrupole-ion trap (LTQ-IT) mass spectrometry/mass spectrometry (MS/MS) combined with multivariate statistical analysis. In partial least squares discriminant analysis (PLS-DA), the 62 species clustered depending on their phylogenetic family, in particular, Aceraceae, Betulaceae, and Fagaceae were distinguished from Rosaceae, Fabaceae, and Asteraceae. Quinic acid, gallic acid, quercetin, quercetin derivatives, kaempferol, and kaempferol derivatives were identified as family-specific metabolites, and were found in relatively high concentrations in Aceraceae, Betulaceae, and Fagaceae. Fagaceae and Asteraceae were selected based on results of PLS-DA and bioactivities to determine the correlation between metabolic differences among plant families and bioactivities. Quinic acid, quercetin, kaempferol, quercetin derivatives, and kaempferol derivatives were found in higher concentrations in Fagaceae than in Asteraceae, and were positively correlated with antioxidant and tyrosinase inhibition activities. These results suggest that metabolite profiling was a useful tool for finding the different metabolic states of each plant family and understanding the correlation between metabolites and bioactivities in accordance with plant family.

Isolation and Characterization of Plant Growth-Promoting Endophytic Bacteria Paenibacillus polymyxa SK1 from Lilium lancifolium

“…Metabolite Profiling. UHPLC-LTQ-IT-MS/MS analysis was performed using the method partially adapted from Lee et al [25]. e ermo Fisher Scientific LTQ XL linear ion trap mass spectrometry consisted of an electrospray interface ( ermo Fisher Scientific, San José, CA, USA) coupled with a DIONEX UltiMate 3000 RS Pump, RS Autosampler, RS Column Compartment (Dionex Corporation, Sunnyvale, CA, USA) used for secondary metabolite profiling of the extract.…”

Exploring the metabolomic diversity of plant species across spatial (leaf and stem) components and phylogenic groups

“…The chemical composition of the different plant parts is affected by multiple factors including genetics, metabolic factors, and geo-climatic conditions [14]. Previously, we have highlighted the importance of mass spectrometry (MS)-based metabolomics to identify the chemotaxonomic profiles of different plant samples across various genera and families [8]. The untargeted metabolomics followed by multivariate analyses indicated that metabolite profiles varied significantly for the plant samples across different phylogenies and spatial parts irrespective of the variations in geographical location or in the period of sample collection (2011-2015).…”

“…Ushering into the metabolomics era, mass spectrometry (MS)-based metabolite characterization has evolved as an adept methodology to discern chemotaxonomy, metabolic pathways, and phytochemical characterization, complementing the omics-cascade alongside genomics and proteomics [8]. Metabolomics enables unbiased, highthroughput screening and characterization of the metabolite gamut in biological sample extracts through chromatographic separation, high resolution MS, and enhanced detection sensitivity [9][10][11].…”

“…Previously, discriminant metabolites, including the flavonoid contents in plant extracts, have been reported for varying taxonomic orders. However, the spatial metabolic disparity between different plant parts (leaves and stems) has not been deconstructed comprehensively [8,15]. In general, the chemical composition of different plant parts is largely influenced by genetic factors, nutritional status, and geo-climatic conditions [16].…”

The anti-cancer effect of quercetin in renal cancer through regulating survivin expression and caspase 3 activity

“…Quercetin is a naturally occurring flavonoid and can be extracted from food and plants. Quercetin was found to play anti-tumor effects through regulating tumor cell proliferation and apoptosis [22] . Moreover, quercetin can specifically kill tumor cells with high selectivity and low toxicity to normal cells.…”