Mass spectrometry-based identification of allergens from Curvularia pallescens, a prevalent aerospore in India

Dey, Debarati; Saha, Bodhisattwa; Sircar, Gaurab; Ghosal, Kavita; Bhattacharya, Swati Gupta

doi:10.1016/j.bbapap.2016.03.012

Cited by 17 publications

(11 citation statements)

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“…Detection of the sensitizing aeroallergens is of great importance in diagnosis and management of the disorder. Skin testing is a quick and easy diagnostic tool for allergy diagnosis, and its direct correlation with other in vitro allergy tests (namely total IgE, specific IgE, stripped basophil histamine release) has proved its authenticity [14, 15]. …”

Section: Discussionmentioning

confidence: 99%

Sensitization to Common Aeroallergens in the Atopic Population of West Bengal, India: An Investigation by Skin Prick Test

Dey

Mondal

Laha

et al. 2018

Int Arch Allergy Immunol

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Background: Aeroallergen sensitization is increasing worldwide. The optimal management of allergic disease requires the identification of the allergic sensitivities of the patient. Complete data of offending aeroallergens are scant from West Bengal, India. Hence, we employed a retrospective analysis of an atopic population of West Bengal to investigate the rate of sensitivity toward common aeroallergens including pollens, molds and house dust mites. Objectives: The aim of the present study was to evaluate the profile of skin prick test (SPT) reactivity of an atopic population of eastern India. Methods: The prevalence aeroallergen sensitization was investigated by SPT along with a questionnaire survey of patients who visited the Allergy and Asthma Research Center of West Bengal, India, from June 2016 to May 2018. Patients were categorized according to the demographic (age, gender) and clinical aspects. Statistical tests were applied to differentiate between age and gender of the sufferers. Results: 524 out of 605 patients exhibited a positive SPT response toward one or more aeroallergens tested. One-way ANOVA following Tukey’s post hoc test reveals the age group of 15–40 years was more sensitized than that of < 15 years and no male-female discrimination was found using the paired t test. House dust mites, namely Dermatophagoides pteronyssinus and Dermatophagoides farinae, elicited most significant SPT results (80.34 and 84.92%, respectively) followed by Cocos (73.28%) and Azaridacta (57.25%) pollen. The most sensitive mold was Alternaria alternata (26.9%). Allergic rhinitis, bronchial asthma and atopic dermatitis were recorded as the common symptoms. Other influencing factors were family history, nature and season/time of onset and house conditions. Conclusion: Following the increasing trend, house dust mite sensitization still held the dominance like in any other Indian population reported earlier. This study would pave the way to construct a standard and minimal SPT panel for the atopic patients of eastern India.

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Section: Discussionmentioning

confidence: 99%

Sensitization to Common Aeroallergens in the Atopic Population of West Bengal, India: An Investigation by Skin Prick Test

Dey

Mondal

Laha

et al. 2018

Int Arch Allergy Immunol

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“…The third cell from the base looks swollen compared to the cells around it. Its texture looks smooth and pale brown to brown (Dey et al 2016).…”

Section: Curvularia Pallescens Boedijnmentioning

confidence: 99%

Seed-borne pathogenic fungi on some soybean varieties

et al. 2020

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Abstract. Soesanto L, Hartono ARR, Mugiastuti E, Widarta H. 2020. Seed-borne pathogenic fungi on some soybean varieties. Biodiversitas 21: 4010-4015. The present study was conducted to detect and identify seed-borne pathogenic fungi in some soybean varieties and their effect on seed germination. Experiment was performed in a completely random design with eight treatments and four replicates. Eight soybean varieties i.e., Malabar, Kaba, Dering, Detam I, Sinabung, Dena, Gepak Kuning, and Slamet were selected for the investigation. Seed borne fungi were isolated using blotter test and agar plate techniques. The variables observed were morphological/cultural characteristics, microscopic features of fungi, and percentage of seed germination. A total of eight fungi namely Aspergillus flavus Link, Aspergillus niger van Tieghem, Cladosporium oxysporum Berk. & M.A. Curtis, Colletotrichum dematium (Pers. et Fr.) Grove f.sp. truncate (Schw.) Arx, Curvularia pallescens Boedijn, Fusarium solani (Mart.) Sacc., Melanospora zamiae Corda, and Nigrospora sp. Mason were isolated from different varieties of soybean seeds. The highest seed germination was found to be 80.5% in Daring and Detam I varieties, respectively, and the lowest 53% was recorded in Dena variety.

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“…Apart from this a number of fungi belonging to the species Curvularia are also identified as rice pathogens, including Curvularia pallescens (Almaguer et al, 2013). The phytopathogenic fungus Curvularia pallescens consists of several alkaloids and proteins, such as curvupallides A, B and C, which possess an unusual α,β-unsaturated ene-amide γ-lactam, which might have played a vital role in the reduction of Ag + ions (Abraham et al, 1995, Mukherjee et al, 2001, Dey et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Bioengineered silver nanoparticles using Curvularia pallescens and its fungicidal activity against Cladosporium fulvum

Elgorban

El-Samawaty

Abd‐Elkader

et al. 2017

Saudi Journal of Biological Sciences

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Microorganisms based biosynthesis of nanomaterials has triggered significant attention, due to their great potential as vast source of the production of biocompatible nanoparticles (NPs). Such biosynthesized functional nanomaterials can be used for various biomedical applications. The present study investigates the green synthesis of silver nanoparticles (Ag NPs) using the fungus Curvularia pallescens (C. pallescens) which is isolated from cereals. The C. pallescens cell filtrate was used for the reduction of AgNO3 to Ag NPs. To the best of our knowledge C. pallescens is utilized first time for the preparation of Ag NPs. Several alkaloids and proteins present in the phytopathogenic fungus C. pallescens were mainly responsible for the formation of highly crystalline Ag NPs. The as-synthesized Ag NPs were characterized by using UV–Visible spectroscopy, X-ray diffraction and transmission electron microscopy (TEM). The TEM micrographs have revealed that spherical shaped Ag NPs with polydisperse in size were obtained. These results have clearly suggested that the biomolecules secreted by C. pallescens are mainly responsible for the formation and stabilization of nanoparticles. Furthermore, the antifungal activity of the as-prepared Ag NPs was tested against Cladosporium fulvum, which is the major cause of a serious plant disease, known as tomato leaf mold. The synthesized Ag NPs displayed excellent fungicidal activity against the tested fungal pathogen. The extreme zone of reduction occurred at 50 μL, whereas, an increase in the reduction activity is observed with increasing the concentration of Ag NPs. These encouraging results can be further exploited by employing the as synthesized Ag NPs against various pathogenic fungi in order to ascertain their spectrum of fungicidal activity.

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Mass spectrometry-based identification of allergens from Curvularia pallescens, a prevalent aerospore in India

Cited by 17 publications

References 50 publications

Sensitization to Common Aeroallergens in the Atopic Population of West Bengal, India: An Investigation by Skin Prick Test

Sensitization to Common Aeroallergens in the Atopic Population of West Bengal, India: An Investigation by Skin Prick Test

Seed-borne pathogenic fungi on some soybean varieties

Bioengineered silver nanoparticles using Curvularia pallescens and its fungicidal activity against Cladosporium fulvum

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