Mass-spectrometric study of carbohydrates

Kochetkov, N. K.; Chizhov, O. S.; Zolotarev, B. M.

doi:10.1007/bf01134228

Cited by 13 publications

(16 citation statements)

References 7 publications

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“…The fragmentation pattern under EIMS conditions allows to localize the position of substitution, based on the well investigated fragmentation behavior of the permethylated ground structures. [28][29][30][31] GLC-MS also favors the detection and identification of small side-products and artefacts. A further profit of GLC analysis is the connection with the flame ionization detector (FID).…”

Section: Nmr Spectroscopymentioning

confidence: 99%

Structure analysis of 1,4-glucan derivatives

Mischnick

Heinrich

Gohdes

et al. 2000

Macromol. Chem. Phys.

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Cellulose, starch and cyclodextrin ethers and esters are produced for many fields of application as for example oil recovery, building and ceramic materials, textile and paper industry, food, cosmetics, and pharmaceuticals. For further development of new types of derivatives and also for a better understanding of their properties a detailed analysis of the usually complex mixtures is required. By complete degradation, separation and quantification of appropriate monomer derivatives the regioselectivity in the glucose unit can be determined. For the determination of the substitution pattern in the polymer chain partial random degradation, oligomer analysis by mass spectrometry, and subsequent statistical evaluation can be applied. The composition of products obtained by enzymatic degradation also reflects the pattern in the polymer chain. In addition a possible heterogeneity of the material must be taken into consideration, since any degradation averages the structural information.

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Section: Nmr Spectroscopymentioning

confidence: 99%

Structure analysis of 1,4-glucan derivatives

Mischnick

Heinrich

Gohdes

et al. 2000

Macromol. Chem. Phys.

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“…Mass spectrometers used were a JEOL DX-300 mass spectrometer (CI-MS, isobutane) and a Hewlett Packard model 5970B mass selective detector (El-MS. 70 eV). CI-and El-MS fragment ions [A, J and alditol (ald)] were used to determine the structure of the methylated ohgosaccharide-alditols (22)(23)(24).…”

Section: Methylation Analysismentioning

confidence: 99%

Pectic Polysaccharides from Roots ofGlycyrrhiza uralensis: Possible Contribution of Neutral Oligosaccharides in the Galacturonase-Resistant Region to Anti-Complementary and Mitogenic Activities

Kiyohara¹,

Takemoto²,

Zhao³

et al. 1996

Planta Med

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Digestion with endo-alpha-(1-->4)-polygalacturonase liberated the enzyme-resistant region (PG-1c) as an active site of the anti-complementary and mitogenic pectic polysaccharide (GR-2IIc) from Glycyrrhiza uralensis. Partial acid hydrolysis of PG-1c resulted in acidic oligosaccharides, and methylation analysis and GC-MS analysis of the acidic oligosaccharides suggested that PG-1c comprised a rhamnogalacturonan core such as -->2)-Rha-(1-->4)-GalA-(1-->2)-Rha-(1-->4)-GalA-(1-->-->4)-GalA-(1-->4) as the acidic moiety. Degradation of uronic acids by lithium decreased the anti-complementary and mitogenic activities of PG-1c. Although the products from PG-1c were still active, the methylglycoside of alpha-L-Rha-(1-->4)-alpha-D-GalA-(1-->2)-alpha-L-Rha-(1-->4)-alpha-D-Gal A did not show both activities. The products obtained by the lithium degradation from PG-1c gave fractions containing various neutral oligosaccharide-alditols. Among these fractions the longest and the short oligosaccharide-alditol fractions had relatively potent anti-complementary activity, whereas all oligosaccharide-alditol fractions expressed weak but significant mitogenic activity. GC-MS analysis indicated that the short oligosaccharide-alditol fraction contained various kinds of di- to tetrasaccharide-alditols. However, malto-oligosaccharide-alditols, and malto-, isomalto-, and laminari-oligosaccharides did not show anti-complementary and/or mitogenic activities, and these results suggested that certain neutral carbohydrate chains in PG-1c were responsible for the expression of mitogenic activity as well as anti-complementary activity of PG-1c.

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“…Mass spectra of acetylated compounds were obtained for four species (Table 1). They all show, besides the fragmentation of the acetylated aglycone, strong peaks at mle 259, 199, 139, and 97, suggesting the presence of a triacetylated pentose (9). Indeed, xylose was identified as the only sugar of the two main glycosides of Chrysolina coerulans and arabinose as the main sugar of Dlochrysa fastuosa glycosides.…”

mentioning

confidence: 99%

Cardiac Glycosides in the Defensive Secretion of Chrysomelid Beetles: Evidence for Their Production by the Insects

Pasteels

Daloze

1977

Science

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The defensive secretions of some chrysomelid beetles belonging to the genera Chrysolina, Chrysochloa, and Dlochrysa contain complex mixtures of cardenolides. The spectral data for some of these compounds suggest that they are monohydroxylated digitoxigenin derivatives linked to a pentose (such as xylose or arabinose). Evidence indicates that the beetles do not sequester these steroid glycosides from their host plants.

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Mass-spectrometric study of carbohydrates

Cited by 13 publications

References 7 publications

Structure analysis of 1,4-glucan derivatives

Structure analysis of 1,4-glucan derivatives

Pectic Polysaccharides from Roots ofGlycyrrhiza uralensis: Possible Contribution of Neutral Oligosaccharides in the Galacturonase-Resistant Region to Anti-Complementary and Mitogenic Activities

Cardiac Glycosides in the Defensive Secretion of Chrysomelid Beetles: Evidence for Their Production by the Insects

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