Mass spectrometric detection of nodularin and desmethylnodularin in mussels and flounders

Karlsson, Krister; Sipiä, Vesa O.; Kankaanpää, Harri; Meriluoto, Jussi

doi:10.1016/s1570-0232(02)00802-4

Cited by 56 publications

(52 citation statements)

References 21 publications

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“…In flounder, caught before the peak of the toxic bloom, NOD content in the liver was over 30 times higher than in muscles. This finding is in agreement with earlier reports, e.g., by Sipiä et al (2001Sipiä et al ( , 2006, Karlsson et al (2003), and Kankaanpää et al (2005). The authors measured NOD content in flounder liver within the range of 20 to 2,230 ng g -1 , and much lower in muscles (less than 200 ng g -1 ).…”

Section: Production and Accumulation Of Nodularinsupporting

confidence: 93%

“…On the other hand, high N. spumigena biomass and high NOD concentrations in seston of the coastal waters of the Gulf of Gdańsk in 2009 and 2011 were reflected in higher NOD content in blue mussels collected in the same area. Generally, the NOD content in the soft tissue of the blue mussels was within the range of the values reported from the Baltic Sea in other studies (from 20 to 2,150 ng NOD g -1 ) (e.g., Sipiä et al, 2001;Karlsson et al, 2003;MazurMarzec et al, 2007). We found that smaller mussels tend to accumulate higher amounts of NOD.…”

Section: Production and Accumulation Of Nodularinsupporting

confidence: 86%

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Occurrence of cyanobacteria and cyanotoxin in the Southern Baltic Proper. Filamentous cyanobacteria versus single-celled picocyanobacteria

et al. 2012

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In the Baltic Sea, cyanobacterial community is mainly composed of filamentous nitrogen-fixing forms, including the toxic Nodularia spumigena, and single-celled picocyanobacteria (Pcy), represented by Synechococcus spp. The main aim of the work was to test the hypothesis that the picocyanobacteria dependend on the presence of the nitrogen-fixing cyanobacteria. In addition, the contamination of blue mussels and fish with nodularin (NOD), the N. spumigena toxin, was examined. In years 2008-2011, the samples for the study were collected in the Southern Baltic Proper using FerryBox system and, occasionally, during research cruises. The analyses showed no correlation between the growth of the nitrogen-fixing cyanobacteria and Synechococcus. Compared with the previously published data, a shift in the composition of Pcy phenotypes was observed. This shift might be an indication of the proceeding changes induced by the reduced nutrient loading and/or climate change. Analyses of NOD revealed differences in the cyanotoxin concentrations between mussels of different shell size. The highest concentration of NOD was detected in the liver of round goby. However, temporarily, also the fish muscles were significantly contaminated with the toxin.

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Section: Production and Accumulation Of Nodularinsupporting

confidence: 93%

Section: Production and Accumulation Of Nodularinsupporting

confidence: 86%

Occurrence of cyanobacteria and cyanotoxin in the Southern Baltic Proper. Filamentous cyanobacteria versus single-celled picocyanobacteria

et al. 2012

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“…The conjugation of xenobiotics to GSH increases their polarity and enhances their excretion from the organism. However, later studies by Karlsson et al (2003Karlsson et al ( , 2005, Lehtonen et al (2003), and also our analyses with Hybrid Quadrupole-TOF LC-MS/MS did not confirm the presence of NOD-GSH or NOD-Cys in aquatic animal tissues.…”

Section: Blue Musselscontrasting

confidence: 78%

“…Karlsson et al (2003), using triple quadrupole and ion trap mass spectrometers, proved that both NOD and the demethylated NOD analogue, presumably [d-Asp 1 ]NOD, were present in Baltic mussel tissue and flounder liver. In the profile of TIC related to sediment and mussel extracts, relatively high intensity peaks of [DMAdda 3 ]NOD compared to the peak of NOD were observed.…”

Section: Fishmentioning

confidence: 99%

Accumulation of nodularin in sediments, mussels, and fish from the Gulf of Gdańsk, southern Baltic Sea

Mazur‐Marzec

Tymińska

Szafranek

et al. 2007

Environmental Toxicology

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In the Gulf of Gdań sk, as in other parts of the Baltic Sea, toxic blooms of Nodularia spumigena are an annual phenomenon. In the present work, the accumulation of nodularin (NOD), a cyanobacterial pentapeptide hepatotoxin, in sediments, blue mussels, and flounders from the Gulf of Gdań sk was studied by enzyme-linked immunosorbent assay (ELISA). In the surface layers of the sediments NOD concentration ranged from 2.3 ng/g dry weight (dw) several months after cyanobacterial bloom to 75 ng/g dw during the bloom. The highest toxin content in mussels was 139 ng/g dw. In two sampling stations situated in the coastal waters of the Gulf of Gdań sk the concentrations of NOD in sediments and mussels were significantly lower than those measured in the Gulf of Finland. In sediments and mussels collected in the Gulf of Gdań sk, the toxin was also detected in March when N. spumigena did not occur. In flounder, NOD accumulated in the liver (489 ng/g dw), guts (21 ng/g dw), and gonads (21 ng/g dw). Hybride quadrupole-time-of-flight liquid chromatography/mass spectrometry/mass spectrometry (TOF-LC/MS/MS) confirmed the presence of NOD in sediment, mussel, and fish samples. Additionally, other NOD analogues, ([DMAdda 3 ]NOD and [dhb 5 ]NOD), were detected in sediments and mussel tissue. No NOD conjugates with reduced glutathione or cysteine were found in fish and mussels. #

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“…MC-LR is a heptapeptide with the chemical structure cyclo ), Scheme 1B, in which Mdhb is 2-(methylamino)-2-dehydrobutyric acid [4]. In the chemical structures of MC-LR and NOD, Adda corresponds to the unique C 20 b-amino acid (3-amino-9-methoxy-2,6,8-trimethyl-10-phenyldeca-4,6-dienoic acid) [5].…”

Section: Introductionmentioning

confidence: 99%

DNA – Cyanobacterial Hepatotoxins Microcystin‐LR and Nodularin Interaction: Electrochemical Evaluation

et al. 2012

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Microcystin-LR (MC-LR) and nodularin (NOD), two potent cyanotoxins with strong hepatotoxic, genotoxic and carcinogenic potential have been associated with the induction of deoxyribonucleic acid (DNA) damage in vitro and in vivo. Electrochemical studies were performed to understand the DNA interaction mechanisms with MC-LR and NOD using a dsDNA-electrochemical biosensor and incubated solutions. The decrease of the dsDNA oxidation peaks with increasing incubation time due to aggregation of DNA strands and the liberation of adenine free residues, causing the occurrence of DNA abasic sites, was observed, which may introduce mutations in the dsDNA during the replication process.

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Mass spectrometric detection of nodularin and desmethylnodularin in mussels and flounders

Cited by 56 publications

References 21 publications

Occurrence of cyanobacteria and cyanotoxin in the Southern Baltic Proper. Filamentous cyanobacteria versus single-celled picocyanobacteria

Occurrence of cyanobacteria and cyanotoxin in the Southern Baltic Proper. Filamentous cyanobacteria versus single-celled picocyanobacteria

Accumulation of nodularin in sediments, mussels, and fish from the Gulf of Gdańsk, southern Baltic Sea

DNA – Cyanobacterial Hepatotoxins Microcystin‐LR and Nodularin Interaction: Electrochemical Evaluation

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