Mass Spectrometric Characterization of Oligo- and Polysaccharides and Their Derivatives

Mischnick, Petra

doi:10.1007/12_2011_134

Cited by 22 publications

(13 citation statements)

References 181 publications

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“…Fast-atom-bombardment MS (FAB MS), matrix-assisted laser-desorption-ionization time-of-flight MS (MALDI TOF MS), and electrospray ionization MS (ESI MS) have become key techniques for identification and molecular mass profiling of polysaccharides present in complex mixtures. Tandem mass (MS/MS) or even MS n spectrometry can give more detailed structural information including sugar constituents, sequence and interresidue linkage positions, and some information on stereochemistry (Mischnick, 2012). To reduce the complexity of the analyte, isolation and profiling of analyte mixtures and even isobaric analytes can be achieved by coupling techniques.…”

Section: Structural Analysismentioning

confidence: 99%

A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications

Liu

Willför

2015

Bioactive Carbohydrates and Dietary Fibre

508

202

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Section: Structural Analysismentioning

confidence: 99%

A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications

Liu

Willför

2015

Bioactive Carbohydrates and Dietary Fibre

508

202

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“…In addition, the increasing biological importance of saccharides is difficult to detect because their lack an ionization natural and low sensitivity in mass spectrometry than other biomass (Zaia 2010;Chang et al, 2011a;Harvey, 2011;Mischnick 2011). The development of methods for mass spectrometry of isolated glycan from glycoconjugates and polysaccharides are commonly used chemical operation to form the tagged or/and permethylated glycan for mass determination and structural analysis (Fig.…”

Section: Introductionmentioning

confidence: 99%

Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

Yang¹

2012

Tandem Mass Spectrometry - Applications and Principles

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“…Correct molar ratios of analytes belonging to the DS profile of a certain DP are the basic requirement for a reliable substituent pattern analysis. To determine the substitution pattern or, where required, block length of multi-block structures [ 20 ], depolymerization into shorter oligosaccharides is a necessary step which is usually performed by either partial acid hydrolysis (Fig. 2 B), or mild enzymatic degradation in case of polysaccharides bearing sensitive substituents such as ester groups.…”

Section: Polysaccharides: Quantifying Substituent Patternsmentioning

confidence: 99%

Biomacromolecules as tools and objects in nanometrology—current challenges and perspectives

et al. 2017

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Nucleic acids, proteins, and polysaccharides are the most important classes of biopolymers. The inherent properties of biomacromolecules are contrary to those of well-defined small molecules consequently raising a number of specific challenges which become particularly apparent if biomacromolecules are treated as objects in quantitative analysis. At the same time, their specific functional ability of molecular recognition and self-organization (e.g., enzymes, antibodies, DNA) enables us to make biomacromolecules serving as molecular tools in biochemistry and molecular biology, or as precisely controllable dimensional platforms for nanometrological applications. Given the complexity of biomacromolecules, quantitative analysis is not limited to the measurement of their concentration but also involves the determination of numerous descriptors related to structure, interaction, activity, and function. Among the biomacromolecules, glycans set examples that quantitative characterization is not necessarily directed to the measurement of amount-of-substance concentration but instead involves the determination of relative proportions (molar ratios) of structural features for comparison with theoretical models. This article addresses current activities to combine optical techniques such as Raman spectroscopy with isotope dilution approaches to realize reference measurement procedures for the quantification of protein biomarkers as an alternative to mass spectrometry-based techniques. Furthermore, it is explored how established ID-MS protocols are being modified to make them applicable for quantifying virus proteins to measure the HIV viral load in blood samples. As an example from the class of carbohydrates, the challenges in accurate determination of substitution patterns are outlined and discussed. Finally, it is presented that biomacromolecules can also serve as tools in quantitative measurements of dimensions with an example of DNA origami to generate defined dimensional standards to be used for calibration in super-resolution fluorescence microscopy. Graphical abstractQuantitative analysis of biomacromolecules is accompanied with special challenges different from low molecular weight compounds. In addition, they are not only objects but also tools applicable for quantitative measurements

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Mass Spectrometric Characterization of Oligo- and Polysaccharides and Their Derivatives

Cited by 22 publications

References 181 publications

A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications

A review of bioactive plant polysaccharides: Biological activities, functionalization, and biomedical applications

Tandem Mass Spectrometry of Tagged and Permethylated Polysaccharides

Biomacromolecules as tools and objects in nanometrology—current challenges and perspectives

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